Cargando…

A biomimetic nanoparticle-enabled toxoid vaccine against melittin

BACKGROUND: Melittin, the main active peptide ingredient of bee venom, can cause severe cell membrane lysis due to its robust interaction with negatively charged phospholipids. So far, no effective anti-melittin vaccine has been developed to protect people from undesired melittin intoxication. METHO...

Descripción completa

Detalles Bibliográficos
Autores principales: Kang, Tianyi, Li, Chenyang, Du, Ting, Wu, Yujiao, Yang, Yuping, Liu, Xuan, Zhang, Qianqian, Xu, Xiaoping, Gou, Maling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987856/
https://www.ncbi.nlm.nih.gov/pubmed/29910613
http://dx.doi.org/10.2147/IJN.S156346
_version_ 1783329193977708544
author Kang, Tianyi
Li, Chenyang
Du, Ting
Wu, Yujiao
Yang, Yuping
Liu, Xuan
Zhang, Qianqian
Xu, Xiaoping
Gou, Maling
author_facet Kang, Tianyi
Li, Chenyang
Du, Ting
Wu, Yujiao
Yang, Yuping
Liu, Xuan
Zhang, Qianqian
Xu, Xiaoping
Gou, Maling
author_sort Kang, Tianyi
collection PubMed
description BACKGROUND: Melittin, the main active peptide ingredient of bee venom, can cause severe cell membrane lysis due to its robust interaction with negatively charged phospholipids. So far, no effective anti-melittin vaccine has been developed to protect people from undesired melittin intoxication. METHODS: Herein, we prepared a polydiacetylene (PDA) nanoparticle with cell membrane-mimic surface to complex melittin, forming an anti-melittin vaccine (PDA–melittin). RESULTS: PDA nanoparticles could effectively combine with melittin and neutralize its toxicity. PDA–melittin nanocomplex is demonstrated to enhance melittin uptake by DCs and stimulate strong melittin-specific immunity. Mice immunized with PDA–melittin nanocomplex showed higher survival rate after exposion to melittin than untreated mice. CONCLUSION: The PDA–melittin nanocomplex can efficiently and safely generate a specific immunity against melittin to protect body from melittin intoxication, providing a new method with potential clinical application for the treatment of melittin intoxication.
format Online
Article
Text
id pubmed-5987856
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Dove Medical Press
record_format MEDLINE/PubMed
spelling pubmed-59878562018-06-15 A biomimetic nanoparticle-enabled toxoid vaccine against melittin Kang, Tianyi Li, Chenyang Du, Ting Wu, Yujiao Yang, Yuping Liu, Xuan Zhang, Qianqian Xu, Xiaoping Gou, Maling Int J Nanomedicine Original Research BACKGROUND: Melittin, the main active peptide ingredient of bee venom, can cause severe cell membrane lysis due to its robust interaction with negatively charged phospholipids. So far, no effective anti-melittin vaccine has been developed to protect people from undesired melittin intoxication. METHODS: Herein, we prepared a polydiacetylene (PDA) nanoparticle with cell membrane-mimic surface to complex melittin, forming an anti-melittin vaccine (PDA–melittin). RESULTS: PDA nanoparticles could effectively combine with melittin and neutralize its toxicity. PDA–melittin nanocomplex is demonstrated to enhance melittin uptake by DCs and stimulate strong melittin-specific immunity. Mice immunized with PDA–melittin nanocomplex showed higher survival rate after exposion to melittin than untreated mice. CONCLUSION: The PDA–melittin nanocomplex can efficiently and safely generate a specific immunity against melittin to protect body from melittin intoxication, providing a new method with potential clinical application for the treatment of melittin intoxication. Dove Medical Press 2018-06-01 /pmc/articles/PMC5987856/ /pubmed/29910613 http://dx.doi.org/10.2147/IJN.S156346 Text en © 2018 Kang et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Kang, Tianyi
Li, Chenyang
Du, Ting
Wu, Yujiao
Yang, Yuping
Liu, Xuan
Zhang, Qianqian
Xu, Xiaoping
Gou, Maling
A biomimetic nanoparticle-enabled toxoid vaccine against melittin
title A biomimetic nanoparticle-enabled toxoid vaccine against melittin
title_full A biomimetic nanoparticle-enabled toxoid vaccine against melittin
title_fullStr A biomimetic nanoparticle-enabled toxoid vaccine against melittin
title_full_unstemmed A biomimetic nanoparticle-enabled toxoid vaccine against melittin
title_short A biomimetic nanoparticle-enabled toxoid vaccine against melittin
title_sort biomimetic nanoparticle-enabled toxoid vaccine against melittin
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987856/
https://www.ncbi.nlm.nih.gov/pubmed/29910613
http://dx.doi.org/10.2147/IJN.S156346
work_keys_str_mv AT kangtianyi abiomimeticnanoparticleenabledtoxoidvaccineagainstmelittin
AT lichenyang abiomimeticnanoparticleenabledtoxoidvaccineagainstmelittin
AT duting abiomimeticnanoparticleenabledtoxoidvaccineagainstmelittin
AT wuyujiao abiomimeticnanoparticleenabledtoxoidvaccineagainstmelittin
AT yangyuping abiomimeticnanoparticleenabledtoxoidvaccineagainstmelittin
AT liuxuan abiomimeticnanoparticleenabledtoxoidvaccineagainstmelittin
AT zhangqianqian abiomimeticnanoparticleenabledtoxoidvaccineagainstmelittin
AT xuxiaoping abiomimeticnanoparticleenabledtoxoidvaccineagainstmelittin
AT goumaling abiomimeticnanoparticleenabledtoxoidvaccineagainstmelittin
AT kangtianyi biomimeticnanoparticleenabledtoxoidvaccineagainstmelittin
AT lichenyang biomimeticnanoparticleenabledtoxoidvaccineagainstmelittin
AT duting biomimeticnanoparticleenabledtoxoidvaccineagainstmelittin
AT wuyujiao biomimeticnanoparticleenabledtoxoidvaccineagainstmelittin
AT yangyuping biomimeticnanoparticleenabledtoxoidvaccineagainstmelittin
AT liuxuan biomimeticnanoparticleenabledtoxoidvaccineagainstmelittin
AT zhangqianqian biomimeticnanoparticleenabledtoxoidvaccineagainstmelittin
AT xuxiaoping biomimeticnanoparticleenabledtoxoidvaccineagainstmelittin
AT goumaling biomimeticnanoparticleenabledtoxoidvaccineagainstmelittin