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A novel mouse model of Campylobacter jejuni enteropathy and diarrhea

Campylobacter infections are among the leading bacterial causes of diarrhea and of ‘environmental enteropathy’ (EE) and growth failure worldwide. However, the lack of an inexpensive small animal model of enteric disease with Campylobacter has been a major limitation for understanding its pathogenesi...

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Autores principales: Giallourou, Natasa, Medlock, Gregory L., Bolick, David T., Medeiros, Pedro HQS, Ledwaba, Solanka E., Kolling, Glynis L., Tung, Kenneth, Guerry, Patricia, Swann, Jonathan R., Guerrant, Richard L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5988333/
https://www.ncbi.nlm.nih.gov/pubmed/29791507
http://dx.doi.org/10.1371/journal.ppat.1007083
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author Giallourou, Natasa
Medlock, Gregory L.
Bolick, David T.
Medeiros, Pedro HQS
Ledwaba, Solanka E.
Kolling, Glynis L.
Tung, Kenneth
Guerry, Patricia
Swann, Jonathan R.
Guerrant, Richard L.
author_facet Giallourou, Natasa
Medlock, Gregory L.
Bolick, David T.
Medeiros, Pedro HQS
Ledwaba, Solanka E.
Kolling, Glynis L.
Tung, Kenneth
Guerry, Patricia
Swann, Jonathan R.
Guerrant, Richard L.
author_sort Giallourou, Natasa
collection PubMed
description Campylobacter infections are among the leading bacterial causes of diarrhea and of ‘environmental enteropathy’ (EE) and growth failure worldwide. However, the lack of an inexpensive small animal model of enteric disease with Campylobacter has been a major limitation for understanding its pathogenesis, interventions or vaccine development. We describe a robust standard mouse model that can exhibit reproducible bloody diarrhea or growth failure, depending on the zinc or protein deficient diet and on antibiotic alteration of normal microbiota prior to infection. Zinc deficiency and the use of antibiotics create a niche for Campylobacter infection to establish by narrowing the metabolic flexibility of these mice for pathogen clearance and by promoting intestinal and systemic inflammation. Several biomarkers and intestinal pathology in this model also mimic those seen in human disease. This model provides a novel tool to test specific hypotheses regarding disease pathogenesis as well as vaccine development that is currently in progress.
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spelling pubmed-59883332018-06-16 A novel mouse model of Campylobacter jejuni enteropathy and diarrhea Giallourou, Natasa Medlock, Gregory L. Bolick, David T. Medeiros, Pedro HQS Ledwaba, Solanka E. Kolling, Glynis L. Tung, Kenneth Guerry, Patricia Swann, Jonathan R. Guerrant, Richard L. PLoS Pathog Research Article Campylobacter infections are among the leading bacterial causes of diarrhea and of ‘environmental enteropathy’ (EE) and growth failure worldwide. However, the lack of an inexpensive small animal model of enteric disease with Campylobacter has been a major limitation for understanding its pathogenesis, interventions or vaccine development. We describe a robust standard mouse model that can exhibit reproducible bloody diarrhea or growth failure, depending on the zinc or protein deficient diet and on antibiotic alteration of normal microbiota prior to infection. Zinc deficiency and the use of antibiotics create a niche for Campylobacter infection to establish by narrowing the metabolic flexibility of these mice for pathogen clearance and by promoting intestinal and systemic inflammation. Several biomarkers and intestinal pathology in this model also mimic those seen in human disease. This model provides a novel tool to test specific hypotheses regarding disease pathogenesis as well as vaccine development that is currently in progress. Public Library of Science 2018-05-23 /pmc/articles/PMC5988333/ /pubmed/29791507 http://dx.doi.org/10.1371/journal.ppat.1007083 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Giallourou, Natasa
Medlock, Gregory L.
Bolick, David T.
Medeiros, Pedro HQS
Ledwaba, Solanka E.
Kolling, Glynis L.
Tung, Kenneth
Guerry, Patricia
Swann, Jonathan R.
Guerrant, Richard L.
A novel mouse model of Campylobacter jejuni enteropathy and diarrhea
title A novel mouse model of Campylobacter jejuni enteropathy and diarrhea
title_full A novel mouse model of Campylobacter jejuni enteropathy and diarrhea
title_fullStr A novel mouse model of Campylobacter jejuni enteropathy and diarrhea
title_full_unstemmed A novel mouse model of Campylobacter jejuni enteropathy and diarrhea
title_short A novel mouse model of Campylobacter jejuni enteropathy and diarrhea
title_sort novel mouse model of campylobacter jejuni enteropathy and diarrhea
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5988333/
https://www.ncbi.nlm.nih.gov/pubmed/29791507
http://dx.doi.org/10.1371/journal.ppat.1007083
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