Top-down proteomic characterization of DAOY medulloblastoma tumor cell line

The proteome of the DAOY medulloblastoma cell line has been investigated by an LC–MS top-down platform. This approach, unlike bottom-up ones, allows identifying proteins and peptides in their intact/native forms, disclosing post-translational modifications, proteoforms and naturally occurring peptid...

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Detalles Bibliográficos
Autores principales: Martelli, Claudia, D’Angelo, Luca, Barba, Marta, Baranzini, Mirko, Inserra, Ilaria, Iavarone, Federica, Vincenzoni, Federica, Tamburrini, Gianpiero, Massimi, Luca, Rocco, Concezio Di, Caldarelli, Massimo, Messana, Irene, Michetti, Fabrizio, Castagnola, Massimo, Lattanzi, Wanda, Desiderio, Claudia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Special Section: Proceedings of the 9th Annual EuPA Congress “Proteomics - Back to the Future” (June 23 - 28, 2015, Milano, Italy)
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5988510/
https://www.ncbi.nlm.nih.gov/pubmed/29900115
http://dx.doi.org/10.1016/j.euprot.2016.03.015
Descripción
Sumario:The proteome of the DAOY medulloblastoma cell line has been investigated by an LC–MS top-down platform. This approach, unlike bottom-up ones, allows identifying proteins and peptides in their intact/native forms, disclosing post-translational modifications, proteoforms and naturally occurring peptides. Indeed, 25 out of the 53 proteins identified, were not previously characterized in DAOY cells. Most of them were functionally interconnected, being mainly involved in binding, catalytic and structural activities, and metabolic processes. The top-down approach, applied in this preliminary study, disclosed the presence of several naturally occurring peptide fragments that characterize DAOY cells.

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