Cargando…
Medicarpin induces lipolysis via activation of Protein Kinase A in brown adipocytes
Natural pterocarpan Medicarpin (Med) has been shown to have various beneficial biological roles, including inhibition of osteoclastogenesis, stimulation of bone regeneration and induction of apoptosis. However, the effect of the Med on lipolysis in adipocytes has not been reported. Here, we show the...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society for Biochemistry and Molecular Biology
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5988580/ https://www.ncbi.nlm.nih.gov/pubmed/29353597 http://dx.doi.org/10.5483/BMBRep.2018.51.5.228 |
_version_ | 1783329309035855872 |
---|---|
author | Imran, Khan Mohammad Yoon, Dahyeon Lee, Tae-Jin Kim, Yong-Sik |
author_facet | Imran, Khan Mohammad Yoon, Dahyeon Lee, Tae-Jin Kim, Yong-Sik |
author_sort | Imran, Khan Mohammad |
collection | PubMed |
description | Natural pterocarpan Medicarpin (Med) has been shown to have various beneficial biological roles, including inhibition of osteoclastogenesis, stimulation of bone regeneration and induction of apoptosis. However, the effect of the Med on lipolysis in adipocytes has not been reported. Here, we show the effect of Med on lipolysis in different mouse adipocytes and elucidate the underlying mechanism. We observed that Med treatment promoted release of glycerol in the media. Differentiated mouse brown adipose tissue cells were treated with Med. RNA-Seq analysis was performed to elucidate the effect of med and subsequently was confirmed by qRT-PCR and western blotting analyses. Med treatment increased both protein and gene expression levels of hormone-sensitive lipase (Hsl) and adipose triglyceride lipase (Atgl), which are two critical enzymes necessary for lipolysis. Mechanistic study showed that Med activates Protein Kinase A (PKA) and phosphorylates Hsl at PKA target position at Serine(660). Silencing of PKA gene by short interfering RNA attenuated the Med-induced increase in glycerol release and Hsl phosphorylation. The results unveil that Med boosts lipolysis via a PKA-dependent pathway in adipocytes and may provide a possible avenue of further research of Med mediated reduction of body fat. |
format | Online Article Text |
id | pubmed-5988580 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Korean Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-59885802018-06-12 Medicarpin induces lipolysis via activation of Protein Kinase A in brown adipocytes Imran, Khan Mohammad Yoon, Dahyeon Lee, Tae-Jin Kim, Yong-Sik BMB Rep Articles Natural pterocarpan Medicarpin (Med) has been shown to have various beneficial biological roles, including inhibition of osteoclastogenesis, stimulation of bone regeneration and induction of apoptosis. However, the effect of the Med on lipolysis in adipocytes has not been reported. Here, we show the effect of Med on lipolysis in different mouse adipocytes and elucidate the underlying mechanism. We observed that Med treatment promoted release of glycerol in the media. Differentiated mouse brown adipose tissue cells were treated with Med. RNA-Seq analysis was performed to elucidate the effect of med and subsequently was confirmed by qRT-PCR and western blotting analyses. Med treatment increased both protein and gene expression levels of hormone-sensitive lipase (Hsl) and adipose triglyceride lipase (Atgl), which are two critical enzymes necessary for lipolysis. Mechanistic study showed that Med activates Protein Kinase A (PKA) and phosphorylates Hsl at PKA target position at Serine(660). Silencing of PKA gene by short interfering RNA attenuated the Med-induced increase in glycerol release and Hsl phosphorylation. The results unveil that Med boosts lipolysis via a PKA-dependent pathway in adipocytes and may provide a possible avenue of further research of Med mediated reduction of body fat. Korean Society for Biochemistry and Molecular Biology 2018-05 2018-05-31 /pmc/articles/PMC5988580/ /pubmed/29353597 http://dx.doi.org/10.5483/BMBRep.2018.51.5.228 Text en Copyright © 2018 by the The Korean Society for Biochemistry and Molecular Biology This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Imran, Khan Mohammad Yoon, Dahyeon Lee, Tae-Jin Kim, Yong-Sik Medicarpin induces lipolysis via activation of Protein Kinase A in brown adipocytes |
title | Medicarpin induces lipolysis via activation of Protein Kinase A in brown adipocytes |
title_full | Medicarpin induces lipolysis via activation of Protein Kinase A in brown adipocytes |
title_fullStr | Medicarpin induces lipolysis via activation of Protein Kinase A in brown adipocytes |
title_full_unstemmed | Medicarpin induces lipolysis via activation of Protein Kinase A in brown adipocytes |
title_short | Medicarpin induces lipolysis via activation of Protein Kinase A in brown adipocytes |
title_sort | medicarpin induces lipolysis via activation of protein kinase a in brown adipocytes |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5988580/ https://www.ncbi.nlm.nih.gov/pubmed/29353597 http://dx.doi.org/10.5483/BMBRep.2018.51.5.228 |
work_keys_str_mv | AT imrankhanmohammad medicarpininduceslipolysisviaactivationofproteinkinaseainbrownadipocytes AT yoondahyeon medicarpininduceslipolysisviaactivationofproteinkinaseainbrownadipocytes AT leetaejin medicarpininduceslipolysisviaactivationofproteinkinaseainbrownadipocytes AT kimyongsik medicarpininduceslipolysisviaactivationofproteinkinaseainbrownadipocytes |