Effect of fetal bovine serum in culture media on MS analysis of mesenchymal stromal cells secretome

The analysis of mesenchymal stromal cells secretome is fundamental to identify key players of processes involving these cells. Truly secreted proteins may be difficult to detect in MS based analysis of conditioned media (CM) due to proteins supplemented with fetal bovine serum (FBS). We compared dif...

Descripción completa

Detalles Bibliográficos
Autores principales: Nonnis, Simona, Maffioli, Elisa, Zanotti, Lucia, Santagata, Fabiana, Negri, Armando, Viola, Antonella, Elliman, Stephen, Tedeschi, Gabriella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Special Section: Proceedings of the 9th Annual EuPA Congress “Proteomics - Back to the Future” (June 23 - 28, 2015, Milano, Italy)
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5988613/
https://www.ncbi.nlm.nih.gov/pubmed/29900097
http://dx.doi.org/10.1016/j.euprot.2016.01.005
_version_ 1783329315922903040
author Nonnis, Simona
Maffioli, Elisa
Zanotti, Lucia
Santagata, Fabiana
Negri, Armando
Viola, Antonella
Elliman, Stephen
Tedeschi, Gabriella
author_facet Nonnis, Simona
Maffioli, Elisa
Zanotti, Lucia
Santagata, Fabiana
Negri, Armando
Viola, Antonella
Elliman, Stephen
Tedeschi, Gabriella
author_sort Nonnis, Simona
collection PubMed
description The analysis of mesenchymal stromal cells secretome is fundamental to identify key players of processes involving these cells. Truly secreted proteins may be difficult to detect in MS based analysis of conditioned media (CM) due to proteins supplemented with fetal bovine serum (FBS). We compared different growth conditions to determine the effect of varying FBS concentration on the number and quantity of truly secreted human proteins vs contaminating bovine proteins. The results suggest that to minimize interference cells should be grown in presence of FBS until confluence and transferred into a serum-free medium prior to secretome collection.
format Online
Article
Text
id pubmed-5988613
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-59886132018-06-13 Effect of fetal bovine serum in culture media on MS analysis of mesenchymal stromal cells secretome Nonnis, Simona Maffioli, Elisa Zanotti, Lucia Santagata, Fabiana Negri, Armando Viola, Antonella Elliman, Stephen Tedeschi, Gabriella EuPA Open Proteom Special Section: Proceedings of the 9th Annual EuPA Congress “Proteomics - Back to the Future” (June 23 - 28, 2015, Milano, Italy) The analysis of mesenchymal stromal cells secretome is fundamental to identify key players of processes involving these cells. Truly secreted proteins may be difficult to detect in MS based analysis of conditioned media (CM) due to proteins supplemented with fetal bovine serum (FBS). We compared different growth conditions to determine the effect of varying FBS concentration on the number and quantity of truly secreted human proteins vs contaminating bovine proteins. The results suggest that to minimize interference cells should be grown in presence of FBS until confluence and transferred into a serum-free medium prior to secretome collection. Elsevier 2016-01-07 /pmc/articles/PMC5988613/ /pubmed/29900097 http://dx.doi.org/10.1016/j.euprot.2016.01.005 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Special Section: Proceedings of the 9th Annual EuPA Congress “Proteomics - Back to the Future” (June 23 - 28, 2015, Milano, Italy)
Nonnis, Simona
Maffioli, Elisa
Zanotti, Lucia
Santagata, Fabiana
Negri, Armando
Viola, Antonella
Elliman, Stephen
Tedeschi, Gabriella
Effect of fetal bovine serum in culture media on MS analysis of mesenchymal stromal cells secretome
title Effect of fetal bovine serum in culture media on MS analysis of mesenchymal stromal cells secretome
title_full Effect of fetal bovine serum in culture media on MS analysis of mesenchymal stromal cells secretome
title_fullStr Effect of fetal bovine serum in culture media on MS analysis of mesenchymal stromal cells secretome
title_full_unstemmed Effect of fetal bovine serum in culture media on MS analysis of mesenchymal stromal cells secretome
title_short Effect of fetal bovine serum in culture media on MS analysis of mesenchymal stromal cells secretome
title_sort effect of fetal bovine serum in culture media on ms analysis of mesenchymal stromal cells secretome
topic Special Section: Proceedings of the 9th Annual EuPA Congress “Proteomics - Back to the Future” (June 23 - 28, 2015, Milano, Italy)
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5988613/
https://www.ncbi.nlm.nih.gov/pubmed/29900097
http://dx.doi.org/10.1016/j.euprot.2016.01.005
work_keys_str_mv AT nonnissimona effectoffetalbovineseruminculturemediaonmsanalysisofmesenchymalstromalcellssecretome
AT maffiolielisa effectoffetalbovineseruminculturemediaonmsanalysisofmesenchymalstromalcellssecretome
AT zanottilucia effectoffetalbovineseruminculturemediaonmsanalysisofmesenchymalstromalcellssecretome
AT santagatafabiana effectoffetalbovineseruminculturemediaonmsanalysisofmesenchymalstromalcellssecretome
AT negriarmando effectoffetalbovineseruminculturemediaonmsanalysisofmesenchymalstromalcellssecretome
AT violaantonella effectoffetalbovineseruminculturemediaonmsanalysisofmesenchymalstromalcellssecretome
AT ellimanstephen effectoffetalbovineseruminculturemediaonmsanalysisofmesenchymalstromalcellssecretome
AT tedeschigabriella effectoffetalbovineseruminculturemediaonmsanalysisofmesenchymalstromalcellssecretome

Ejemplares similares