A novel regulation mechanism of the T7 RNA polymerase based expression system improves overproduction and folding of membrane proteins

Membrane protein (MP) overproduction is one of the major bottlenecks in structural genomics and biotechnology. Despite the emergence of eukaryotic expression systems, bacteria remain a cost effective and powerful tool for protein production. The T7 RNA polymerase (T7RNAP)-based expression system is...

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Detalles Bibliográficos
Autores principales: Angius, Federica, Ilioaia, Oana, Amrani, Amira, Suisse, Annabelle, Rosset, Lindsay, Legrand, Amélie, Abou-Hamdan, Abbas, Uzan, Marc, Zito, Francesca, Miroux, Bruno
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5988807/
https://www.ncbi.nlm.nih.gov/pubmed/29872064
http://dx.doi.org/10.1038/s41598-018-26668-y

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