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The dynamics of a Chinese porcine G9P[23] rotavirus production in MA-104 cells and intestines of 3-day-old piglets
Rotavirus A (RVA) G9 genotype is recognized as an emerging genotype which is spreading worldwide, however, our knowledge on pathogenicity of this virus is limited. In this study, porcine RVA strain HN03 was successfully isolated on MA-104 cells, and the isolate was propagated continuously for 7 pass...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Japanese Society of Veterinary Science
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5989024/ https://www.ncbi.nlm.nih.gov/pubmed/29553062 http://dx.doi.org/10.1292/jvms.17-0657 |
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author | WANG, Zhiyan LV, Chaochao XU, Xin LI, Xiangdong YAO, Yali GAO, Xiaojing SUN, Zhe WANG, Yuzhou SUN, Yujie XIAO, Yan TIAN, Kegong |
author_facet | WANG, Zhiyan LV, Chaochao XU, Xin LI, Xiangdong YAO, Yali GAO, Xiaojing SUN, Zhe WANG, Yuzhou SUN, Yujie XIAO, Yan TIAN, Kegong |
author_sort | WANG, Zhiyan |
collection | PubMed |
description | Rotavirus A (RVA) G9 genotype is recognized as an emerging genotype which is spreading worldwide, however, our knowledge on pathogenicity of this virus is limited. In this study, porcine RVA strain HN03 was successfully isolated on MA-104 cells, and the isolate was propagated continuously for 7 passages after a virus cloning at passage 3. The virus titers from 4 to 10 passages ranged from 10(7.1) to 10(8.1) TCID(50)/ml. The growth curve of HN03 strain in cell culture was determined, and the virus production dynamics was confirmed by immunoperoxidase monolayer assay (IPMA). Sequence and phylogenetic analyses based on full-length VP7 and partial VP4 genes indicated that HN03 strain belongs to genotype G9P[23]. In addition, the sixth passage of strain HN03 in cell culture was subjected to 3-day-old piglets. All infected piglets developed severe watery diarrhea within 24 hr post-inoculation (hpi), but recovered from disease after 72 hpi. RVA antigen could be detected by IHC in the cytoplasm of villous enterocytes as early as 2 hr after appearance of clinical symptoms and virus antigen load kept increasing in the next 30 hr. The dynamics of RVA HN03 strain proliferation on cells and in pigs extended our understanding of rotavirus pathogenicity. |
format | Online Article Text |
id | pubmed-5989024 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-59890242018-06-08 The dynamics of a Chinese porcine G9P[23] rotavirus production in MA-104 cells and intestines of 3-day-old piglets WANG, Zhiyan LV, Chaochao XU, Xin LI, Xiangdong YAO, Yali GAO, Xiaojing SUN, Zhe WANG, Yuzhou SUN, Yujie XIAO, Yan TIAN, Kegong J Vet Med Sci Virology Rotavirus A (RVA) G9 genotype is recognized as an emerging genotype which is spreading worldwide, however, our knowledge on pathogenicity of this virus is limited. In this study, porcine RVA strain HN03 was successfully isolated on MA-104 cells, and the isolate was propagated continuously for 7 passages after a virus cloning at passage 3. The virus titers from 4 to 10 passages ranged from 10(7.1) to 10(8.1) TCID(50)/ml. The growth curve of HN03 strain in cell culture was determined, and the virus production dynamics was confirmed by immunoperoxidase monolayer assay (IPMA). Sequence and phylogenetic analyses based on full-length VP7 and partial VP4 genes indicated that HN03 strain belongs to genotype G9P[23]. In addition, the sixth passage of strain HN03 in cell culture was subjected to 3-day-old piglets. All infected piglets developed severe watery diarrhea within 24 hr post-inoculation (hpi), but recovered from disease after 72 hpi. RVA antigen could be detected by IHC in the cytoplasm of villous enterocytes as early as 2 hr after appearance of clinical symptoms and virus antigen load kept increasing in the next 30 hr. The dynamics of RVA HN03 strain proliferation on cells and in pigs extended our understanding of rotavirus pathogenicity. The Japanese Society of Veterinary Science 2018-03-16 2018-05 /pmc/articles/PMC5989024/ /pubmed/29553062 http://dx.doi.org/10.1292/jvms.17-0657 Text en ©2018 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Virology WANG, Zhiyan LV, Chaochao XU, Xin LI, Xiangdong YAO, Yali GAO, Xiaojing SUN, Zhe WANG, Yuzhou SUN, Yujie XIAO, Yan TIAN, Kegong The dynamics of a Chinese porcine G9P[23] rotavirus production in MA-104 cells and intestines of 3-day-old piglets |
title | The dynamics of a Chinese porcine G9P[23] rotavirus production in MA-104 cells and intestines of 3-day-old piglets |
title_full | The dynamics of a Chinese porcine G9P[23] rotavirus production in MA-104 cells and intestines of 3-day-old piglets |
title_fullStr | The dynamics of a Chinese porcine G9P[23] rotavirus production in MA-104 cells and intestines of 3-day-old piglets |
title_full_unstemmed | The dynamics of a Chinese porcine G9P[23] rotavirus production in MA-104 cells and intestines of 3-day-old piglets |
title_short | The dynamics of a Chinese porcine G9P[23] rotavirus production in MA-104 cells and intestines of 3-day-old piglets |
title_sort | dynamics of a chinese porcine g9p[23] rotavirus production in ma-104 cells and intestines of 3-day-old piglets |
topic | Virology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5989024/ https://www.ncbi.nlm.nih.gov/pubmed/29553062 http://dx.doi.org/10.1292/jvms.17-0657 |
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