Cargando…

The dynamics of a Chinese porcine G9P[23] rotavirus production in MA-104 cells and intestines of 3-day-old piglets

Rotavirus A (RVA) G9 genotype is recognized as an emerging genotype which is spreading worldwide, however, our knowledge on pathogenicity of this virus is limited. In this study, porcine RVA strain HN03 was successfully isolated on MA-104 cells, and the isolate was propagated continuously for 7 pass...

Descripción completa

Detalles Bibliográficos
Autores principales: WANG, Zhiyan, LV, Chaochao, XU, Xin, LI, Xiangdong, YAO, Yali, GAO, Xiaojing, SUN, Zhe, WANG, Yuzhou, SUN, Yujie, XIAO, Yan, TIAN, Kegong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5989024/
https://www.ncbi.nlm.nih.gov/pubmed/29553062
http://dx.doi.org/10.1292/jvms.17-0657
_version_ 1783329378908766208
author WANG, Zhiyan
LV, Chaochao
XU, Xin
LI, Xiangdong
YAO, Yali
GAO, Xiaojing
SUN, Zhe
WANG, Yuzhou
SUN, Yujie
XIAO, Yan
TIAN, Kegong
author_facet WANG, Zhiyan
LV, Chaochao
XU, Xin
LI, Xiangdong
YAO, Yali
GAO, Xiaojing
SUN, Zhe
WANG, Yuzhou
SUN, Yujie
XIAO, Yan
TIAN, Kegong
author_sort WANG, Zhiyan
collection PubMed
description Rotavirus A (RVA) G9 genotype is recognized as an emerging genotype which is spreading worldwide, however, our knowledge on pathogenicity of this virus is limited. In this study, porcine RVA strain HN03 was successfully isolated on MA-104 cells, and the isolate was propagated continuously for 7 passages after a virus cloning at passage 3. The virus titers from 4 to 10 passages ranged from 10(7.1) to 10(8.1) TCID(50)/ml. The growth curve of HN03 strain in cell culture was determined, and the virus production dynamics was confirmed by immunoperoxidase monolayer assay (IPMA). Sequence and phylogenetic analyses based on full-length VP7 and partial VP4 genes indicated that HN03 strain belongs to genotype G9P[23]. In addition, the sixth passage of strain HN03 in cell culture was subjected to 3-day-old piglets. All infected piglets developed severe watery diarrhea within 24 hr post-inoculation (hpi), but recovered from disease after 72 hpi. RVA antigen could be detected by IHC in the cytoplasm of villous enterocytes as early as 2 hr after appearance of clinical symptoms and virus antigen load kept increasing in the next 30 hr. The dynamics of RVA HN03 strain proliferation on cells and in pigs extended our understanding of rotavirus pathogenicity.
format Online
Article
Text
id pubmed-5989024
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher The Japanese Society of Veterinary Science
record_format MEDLINE/PubMed
spelling pubmed-59890242018-06-08 The dynamics of a Chinese porcine G9P[23] rotavirus production in MA-104 cells and intestines of 3-day-old piglets WANG, Zhiyan LV, Chaochao XU, Xin LI, Xiangdong YAO, Yali GAO, Xiaojing SUN, Zhe WANG, Yuzhou SUN, Yujie XIAO, Yan TIAN, Kegong J Vet Med Sci Virology Rotavirus A (RVA) G9 genotype is recognized as an emerging genotype which is spreading worldwide, however, our knowledge on pathogenicity of this virus is limited. In this study, porcine RVA strain HN03 was successfully isolated on MA-104 cells, and the isolate was propagated continuously for 7 passages after a virus cloning at passage 3. The virus titers from 4 to 10 passages ranged from 10(7.1) to 10(8.1) TCID(50)/ml. The growth curve of HN03 strain in cell culture was determined, and the virus production dynamics was confirmed by immunoperoxidase monolayer assay (IPMA). Sequence and phylogenetic analyses based on full-length VP7 and partial VP4 genes indicated that HN03 strain belongs to genotype G9P[23]. In addition, the sixth passage of strain HN03 in cell culture was subjected to 3-day-old piglets. All infected piglets developed severe watery diarrhea within 24 hr post-inoculation (hpi), but recovered from disease after 72 hpi. RVA antigen could be detected by IHC in the cytoplasm of villous enterocytes as early as 2 hr after appearance of clinical symptoms and virus antigen load kept increasing in the next 30 hr. The dynamics of RVA HN03 strain proliferation on cells and in pigs extended our understanding of rotavirus pathogenicity. The Japanese Society of Veterinary Science 2018-03-16 2018-05 /pmc/articles/PMC5989024/ /pubmed/29553062 http://dx.doi.org/10.1292/jvms.17-0657 Text en ©2018 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Virology
WANG, Zhiyan
LV, Chaochao
XU, Xin
LI, Xiangdong
YAO, Yali
GAO, Xiaojing
SUN, Zhe
WANG, Yuzhou
SUN, Yujie
XIAO, Yan
TIAN, Kegong
The dynamics of a Chinese porcine G9P[23] rotavirus production in MA-104 cells and intestines of 3-day-old piglets
title The dynamics of a Chinese porcine G9P[23] rotavirus production in MA-104 cells and intestines of 3-day-old piglets
title_full The dynamics of a Chinese porcine G9P[23] rotavirus production in MA-104 cells and intestines of 3-day-old piglets
title_fullStr The dynamics of a Chinese porcine G9P[23] rotavirus production in MA-104 cells and intestines of 3-day-old piglets
title_full_unstemmed The dynamics of a Chinese porcine G9P[23] rotavirus production in MA-104 cells and intestines of 3-day-old piglets
title_short The dynamics of a Chinese porcine G9P[23] rotavirus production in MA-104 cells and intestines of 3-day-old piglets
title_sort dynamics of a chinese porcine g9p[23] rotavirus production in ma-104 cells and intestines of 3-day-old piglets
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5989024/
https://www.ncbi.nlm.nih.gov/pubmed/29553062
http://dx.doi.org/10.1292/jvms.17-0657
work_keys_str_mv AT wangzhiyan thedynamicsofachineseporcineg9p23rotavirusproductioninma104cellsandintestinesof3dayoldpiglets
AT lvchaochao thedynamicsofachineseporcineg9p23rotavirusproductioninma104cellsandintestinesof3dayoldpiglets
AT xuxin thedynamicsofachineseporcineg9p23rotavirusproductioninma104cellsandintestinesof3dayoldpiglets
AT lixiangdong thedynamicsofachineseporcineg9p23rotavirusproductioninma104cellsandintestinesof3dayoldpiglets
AT yaoyali thedynamicsofachineseporcineg9p23rotavirusproductioninma104cellsandintestinesof3dayoldpiglets
AT gaoxiaojing thedynamicsofachineseporcineg9p23rotavirusproductioninma104cellsandintestinesof3dayoldpiglets
AT sunzhe thedynamicsofachineseporcineg9p23rotavirusproductioninma104cellsandintestinesof3dayoldpiglets
AT wangyuzhou thedynamicsofachineseporcineg9p23rotavirusproductioninma104cellsandintestinesof3dayoldpiglets
AT sunyujie thedynamicsofachineseporcineg9p23rotavirusproductioninma104cellsandintestinesof3dayoldpiglets
AT xiaoyan thedynamicsofachineseporcineg9p23rotavirusproductioninma104cellsandintestinesof3dayoldpiglets
AT tiankegong thedynamicsofachineseporcineg9p23rotavirusproductioninma104cellsandintestinesof3dayoldpiglets
AT wangzhiyan dynamicsofachineseporcineg9p23rotavirusproductioninma104cellsandintestinesof3dayoldpiglets
AT lvchaochao dynamicsofachineseporcineg9p23rotavirusproductioninma104cellsandintestinesof3dayoldpiglets
AT xuxin dynamicsofachineseporcineg9p23rotavirusproductioninma104cellsandintestinesof3dayoldpiglets
AT lixiangdong dynamicsofachineseporcineg9p23rotavirusproductioninma104cellsandintestinesof3dayoldpiglets
AT yaoyali dynamicsofachineseporcineg9p23rotavirusproductioninma104cellsandintestinesof3dayoldpiglets
AT gaoxiaojing dynamicsofachineseporcineg9p23rotavirusproductioninma104cellsandintestinesof3dayoldpiglets
AT sunzhe dynamicsofachineseporcineg9p23rotavirusproductioninma104cellsandintestinesof3dayoldpiglets
AT wangyuzhou dynamicsofachineseporcineg9p23rotavirusproductioninma104cellsandintestinesof3dayoldpiglets
AT sunyujie dynamicsofachineseporcineg9p23rotavirusproductioninma104cellsandintestinesof3dayoldpiglets
AT xiaoyan dynamicsofachineseporcineg9p23rotavirusproductioninma104cellsandintestinesof3dayoldpiglets
AT tiankegong dynamicsofachineseporcineg9p23rotavirusproductioninma104cellsandintestinesof3dayoldpiglets