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Identifying and Overcoming Threats to Reproducibility, Replicability, Robustness, and Generalizability in Microbiome Research
The “reproducibility crisis” in science affects microbiology as much as any other area of inquiry, and microbiologists have long struggled to make their research reproducible. We need to respect that ensuring that our methods and results are sufficiently transparent is difficult. This difficulty is...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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American Society for Microbiology
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5989067/ https://www.ncbi.nlm.nih.gov/pubmed/29871915 http://dx.doi.org/10.1128/mBio.00525-18 |
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author | Schloss, Patrick D. |
author_facet | Schloss, Patrick D. |
author_sort | Schloss, Patrick D. |
collection | PubMed |
description | The “reproducibility crisis” in science affects microbiology as much as any other area of inquiry, and microbiologists have long struggled to make their research reproducible. We need to respect that ensuring that our methods and results are sufficiently transparent is difficult. This difficulty is compounded in interdisciplinary fields such as microbiome research. There are many reasons why a researcher is unable to reproduce a previous result, and even if a result is reproducible, it may not be correct. Furthermore, failures to reproduce previous results have much to teach us about the scientific process and microbial life itself. This Perspective delineates a framework for identifying and overcoming threats to reproducibility, replicability, robustness, and generalizability of microbiome research. Instead of seeing signs of a crisis in others’ work, we need to appreciate the technical and social difficulties that limit reproducibility in the work of others as well as our own. |
format | Online Article Text |
id | pubmed-5989067 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-59890672018-06-12 Identifying and Overcoming Threats to Reproducibility, Replicability, Robustness, and Generalizability in Microbiome Research Schloss, Patrick D. mBio Perspective The “reproducibility crisis” in science affects microbiology as much as any other area of inquiry, and microbiologists have long struggled to make their research reproducible. We need to respect that ensuring that our methods and results are sufficiently transparent is difficult. This difficulty is compounded in interdisciplinary fields such as microbiome research. There are many reasons why a researcher is unable to reproduce a previous result, and even if a result is reproducible, it may not be correct. Furthermore, failures to reproduce previous results have much to teach us about the scientific process and microbial life itself. This Perspective delineates a framework for identifying and overcoming threats to reproducibility, replicability, robustness, and generalizability of microbiome research. Instead of seeing signs of a crisis in others’ work, we need to appreciate the technical and social difficulties that limit reproducibility in the work of others as well as our own. American Society for Microbiology 2018-06-05 /pmc/articles/PMC5989067/ /pubmed/29871915 http://dx.doi.org/10.1128/mBio.00525-18 Text en Copyright © 2018 Schloss. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Perspective Schloss, Patrick D. Identifying and Overcoming Threats to Reproducibility, Replicability, Robustness, and Generalizability in Microbiome Research |
title | Identifying and Overcoming Threats to Reproducibility, Replicability, Robustness, and Generalizability in Microbiome Research |
title_full | Identifying and Overcoming Threats to Reproducibility, Replicability, Robustness, and Generalizability in Microbiome Research |
title_fullStr | Identifying and Overcoming Threats to Reproducibility, Replicability, Robustness, and Generalizability in Microbiome Research |
title_full_unstemmed | Identifying and Overcoming Threats to Reproducibility, Replicability, Robustness, and Generalizability in Microbiome Research |
title_short | Identifying and Overcoming Threats to Reproducibility, Replicability, Robustness, and Generalizability in Microbiome Research |
title_sort | identifying and overcoming threats to reproducibility, replicability, robustness, and generalizability in microbiome research |
topic | Perspective |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5989067/ https://www.ncbi.nlm.nih.gov/pubmed/29871915 http://dx.doi.org/10.1128/mBio.00525-18 |
work_keys_str_mv | AT schlosspatrickd identifyingandovercomingthreatstoreproducibilityreplicabilityrobustnessandgeneralizabilityinmicrobiomeresearch |