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Rivers as carriers and potential sentinels for Burkholderia pseudomallei in Laos

Burkholderia pseudomallei, causative agent of the often fatal disease melioidosis, dwells in tropical soils and has been found in freshwater bodies. To investigate whether rivers are potential habitats or carriers for B. pseudomallei and to assess its geographical distribution in Laos, we studied 23...

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Autores principales: Zimmermann, Rosalie E., Ribolzi, Olivier, Pierret, Alain, Rattanavong, Sayaphet, Robinson, Matthew T., Newton, Paul N., Davong, Viengmon, Auda, Yves, Zopfi, Jakob, Dance, David A. B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5989208/
https://www.ncbi.nlm.nih.gov/pubmed/29875361
http://dx.doi.org/10.1038/s41598-018-26684-y
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author Zimmermann, Rosalie E.
Ribolzi, Olivier
Pierret, Alain
Rattanavong, Sayaphet
Robinson, Matthew T.
Newton, Paul N.
Davong, Viengmon
Auda, Yves
Zopfi, Jakob
Dance, David A. B.
author_facet Zimmermann, Rosalie E.
Ribolzi, Olivier
Pierret, Alain
Rattanavong, Sayaphet
Robinson, Matthew T.
Newton, Paul N.
Davong, Viengmon
Auda, Yves
Zopfi, Jakob
Dance, David A. B.
author_sort Zimmermann, Rosalie E.
collection PubMed
description Burkholderia pseudomallei, causative agent of the often fatal disease melioidosis, dwells in tropical soils and has been found in freshwater bodies. To investigate whether rivers are potential habitats or carriers for B. pseudomallei and to assess its geographical distribution in Laos, we studied 23 rivers including the Mekong, applying culture-based detection methods and PCR to water filters and streambed sediments. B. pseudomallei was present in 9% of the rivers in the dry season and in 57% in the rainy season. We found the pathogen exclusively in Southern and Central Laos, and mainly in turbid river water, while sediments were positive in 35% of the B. pseudomallei-positive sites. Our results provide evidence for a heterogeneous temporal and spatial distribution of B. pseudomallei in rivers in Laos with a clear north-south contrast. The seasonal dynamics and predominant occurrence of B. pseudomallei in particle-rich water suggest that this pathogen is washed out with eroded soil during periods of heavy rainfall and transported by rivers, while river sediments do not seem to be permanent habitats for B. pseudomallei. Rivers may thus be useful to assess the distribution and aquatic dispersal of B. pseudomallei and other environmental pathogens in their catchment area and beyond.
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spelling pubmed-59892082018-06-20 Rivers as carriers and potential sentinels for Burkholderia pseudomallei in Laos Zimmermann, Rosalie E. Ribolzi, Olivier Pierret, Alain Rattanavong, Sayaphet Robinson, Matthew T. Newton, Paul N. Davong, Viengmon Auda, Yves Zopfi, Jakob Dance, David A. B. Sci Rep Article Burkholderia pseudomallei, causative agent of the often fatal disease melioidosis, dwells in tropical soils and has been found in freshwater bodies. To investigate whether rivers are potential habitats or carriers for B. pseudomallei and to assess its geographical distribution in Laos, we studied 23 rivers including the Mekong, applying culture-based detection methods and PCR to water filters and streambed sediments. B. pseudomallei was present in 9% of the rivers in the dry season and in 57% in the rainy season. We found the pathogen exclusively in Southern and Central Laos, and mainly in turbid river water, while sediments were positive in 35% of the B. pseudomallei-positive sites. Our results provide evidence for a heterogeneous temporal and spatial distribution of B. pseudomallei in rivers in Laos with a clear north-south contrast. The seasonal dynamics and predominant occurrence of B. pseudomallei in particle-rich water suggest that this pathogen is washed out with eroded soil during periods of heavy rainfall and transported by rivers, while river sediments do not seem to be permanent habitats for B. pseudomallei. Rivers may thus be useful to assess the distribution and aquatic dispersal of B. pseudomallei and other environmental pathogens in their catchment area and beyond. Nature Publishing Group UK 2018-06-06 /pmc/articles/PMC5989208/ /pubmed/29875361 http://dx.doi.org/10.1038/s41598-018-26684-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zimmermann, Rosalie E.
Ribolzi, Olivier
Pierret, Alain
Rattanavong, Sayaphet
Robinson, Matthew T.
Newton, Paul N.
Davong, Viengmon
Auda, Yves
Zopfi, Jakob
Dance, David A. B.
Rivers as carriers and potential sentinels for Burkholderia pseudomallei in Laos
title Rivers as carriers and potential sentinels for Burkholderia pseudomallei in Laos
title_full Rivers as carriers and potential sentinels for Burkholderia pseudomallei in Laos
title_fullStr Rivers as carriers and potential sentinels for Burkholderia pseudomallei in Laos
title_full_unstemmed Rivers as carriers and potential sentinels for Burkholderia pseudomallei in Laos
title_short Rivers as carriers and potential sentinels for Burkholderia pseudomallei in Laos
title_sort rivers as carriers and potential sentinels for burkholderia pseudomallei in laos
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5989208/
https://www.ncbi.nlm.nih.gov/pubmed/29875361
http://dx.doi.org/10.1038/s41598-018-26684-y
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