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Identifying differentially expressed proteins in sorghum cell cultures exposed to osmotic stress
Drought stress triggers remarkable physiological changes and growth impediments, which significantly diminish plant biomass and crop yield. However, certain plant species show notable resilience, maintaining nearly normal yields under severe water deficits. For example, sorghum is a naturally drough...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5989219/ https://www.ncbi.nlm.nih.gov/pubmed/29875393 http://dx.doi.org/10.1038/s41598-018-27003-1 |
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author | Ngara, Rudo Ramulifho, Elelwani Movahedi, Mahsa Shargie, Nemera G. Brown, Adrian P. Chivasa, Stephen |
author_facet | Ngara, Rudo Ramulifho, Elelwani Movahedi, Mahsa Shargie, Nemera G. Brown, Adrian P. Chivasa, Stephen |
author_sort | Ngara, Rudo |
collection | PubMed |
description | Drought stress triggers remarkable physiological changes and growth impediments, which significantly diminish plant biomass and crop yield. However, certain plant species show notable resilience, maintaining nearly normal yields under severe water deficits. For example, sorghum is a naturally drought-tolerant crop, which is ideal for studying plant adaptive responses to drought. Here we used sorbitol treatments to simulate drought-induced osmotic stress in sorghum cell suspension cultures and analysed fractions enriched for extracellular matrix proteins using isobaric tags for relative and absolute quantification technology. Sorbitol induced an overall increase in protein secretion, with putative redox proteins, proteases, and glycosyl hydrolases featuring prominently among the responsive proteins. Gene expression analysis of selected candidates revealed regulation at the transcriptional level. There was a notable differential gene expression between drought-tolerant and drought-sensitive sorghum varieties for some of the candidates. This study shows that protein secretion is a major component of the sorghum response to osmotic stress. Additionally, our data provide candidate genes, which may have putative functions in sorghum drought tolerance, and offer a pool of genes that could be developed as potential biomarkers for rapid identification of drought tolerant lines in plant breeding programs. |
format | Online Article Text |
id | pubmed-5989219 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59892192018-06-20 Identifying differentially expressed proteins in sorghum cell cultures exposed to osmotic stress Ngara, Rudo Ramulifho, Elelwani Movahedi, Mahsa Shargie, Nemera G. Brown, Adrian P. Chivasa, Stephen Sci Rep Article Drought stress triggers remarkable physiological changes and growth impediments, which significantly diminish plant biomass and crop yield. However, certain plant species show notable resilience, maintaining nearly normal yields under severe water deficits. For example, sorghum is a naturally drought-tolerant crop, which is ideal for studying plant adaptive responses to drought. Here we used sorbitol treatments to simulate drought-induced osmotic stress in sorghum cell suspension cultures and analysed fractions enriched for extracellular matrix proteins using isobaric tags for relative and absolute quantification technology. Sorbitol induced an overall increase in protein secretion, with putative redox proteins, proteases, and glycosyl hydrolases featuring prominently among the responsive proteins. Gene expression analysis of selected candidates revealed regulation at the transcriptional level. There was a notable differential gene expression between drought-tolerant and drought-sensitive sorghum varieties for some of the candidates. This study shows that protein secretion is a major component of the sorghum response to osmotic stress. Additionally, our data provide candidate genes, which may have putative functions in sorghum drought tolerance, and offer a pool of genes that could be developed as potential biomarkers for rapid identification of drought tolerant lines in plant breeding programs. Nature Publishing Group UK 2018-06-06 /pmc/articles/PMC5989219/ /pubmed/29875393 http://dx.doi.org/10.1038/s41598-018-27003-1 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Ngara, Rudo Ramulifho, Elelwani Movahedi, Mahsa Shargie, Nemera G. Brown, Adrian P. Chivasa, Stephen Identifying differentially expressed proteins in sorghum cell cultures exposed to osmotic stress |
title | Identifying differentially expressed proteins in sorghum cell cultures exposed to osmotic stress |
title_full | Identifying differentially expressed proteins in sorghum cell cultures exposed to osmotic stress |
title_fullStr | Identifying differentially expressed proteins in sorghum cell cultures exposed to osmotic stress |
title_full_unstemmed | Identifying differentially expressed proteins in sorghum cell cultures exposed to osmotic stress |
title_short | Identifying differentially expressed proteins in sorghum cell cultures exposed to osmotic stress |
title_sort | identifying differentially expressed proteins in sorghum cell cultures exposed to osmotic stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5989219/ https://www.ncbi.nlm.nih.gov/pubmed/29875393 http://dx.doi.org/10.1038/s41598-018-27003-1 |
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