SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology

We report a microfluidic system that physically separates nuclear RNA (nucRNA) and cytoplasmic RNA (cytRNA) from a single cell and enables single-cell integrated nucRNA and cytRNA-sequencing (SINC-seq). SINC-seq constructs two individual RNA-seq libraries, nucRNA and cytRNA, per cell, quantifies gen...

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Detalles Bibliográficos
Autores principales: Abdelmoez, Mahmoud N., Iida, Kei, Oguchi, Yusuke, Nishikii, Hidekazu, Yokokawa, Ryuji, Kotera, Hidetoshi, Uemura, Sotaro, Santiago, Juan G., Shintaku, Hirofumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5989370/
https://www.ncbi.nlm.nih.gov/pubmed/29871653
http://dx.doi.org/10.1186/s13059-018-1446-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5989370/
https://www.ncbi.nlm.nih.gov/pubmed/29871653
http://dx.doi.org/10.1186/s13059-018-1446-9

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