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High-Level Precise Knockin of iPSCs by Simultaneous Reprogramming and Genome Editing of Human Peripheral Blood Mononuclear Cells
We have developed an improved episomal vector system for efficient generation of integration-free induced pluripotent stem cells (iPSCs) from peripheral blood mononuclear cells. More recently, we reported that the use of an optimized CRISPR-Cas9 system together with a double-cut donor increases homo...
Autores principales: | Wen, Wei, Cheng, Xinxin, Fu, Yawen, Meng, Feiying, Zhang, Jian-Ping, Zhang, Lu, Li, Xiao-Lan, Yang, Zhixue, Xu, Jing, Zhang, Feng, Botimer, Gary D., Yuan, Weiping, Sun, Changkai, Cheng, Tao, Zhang, Xiao-Bing |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5989814/ https://www.ncbi.nlm.nih.gov/pubmed/29754960 http://dx.doi.org/10.1016/j.stemcr.2018.04.013 |
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