Multiplexed precision genome editing with trackable genomic barcodes in yeast

Our understanding of how genotype controls phenotype is limited by the scale at which we can precisely alter the genome and assess phenotypic consequences of each perturbation. Here we describe a CRISPR/Cas9-based method for multiplexed accurate genome editing with short, trackable, integrated cellu...

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Detalles Bibliográficos
Autores principales: Roy, Kevin R., Smith, Justin D., Vonesch, Sibylle C., Lin, Gen, Tu, Chelsea Szu, Lederer, Alex R., Chu, Angela, Suresh, Sundari, Nguyen, Michelle, Horecka, Joe, Tripathi, Ashutosh, Burnett, Wallace T., Morgan, Maddison A., Schulz, Julia, Orsley, Kevin M., Wei, Wu, Aiyar, Raeka S., Davis, Ronald W., Bankaitis, Vytas A., Haber, James E., Salit, Marc L., St Onge, Robert P., Steinmetz, Lars M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5990450/
https://www.ncbi.nlm.nih.gov/pubmed/29734294
http://dx.doi.org/10.1038/nbt.4137

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