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A plug-in electrophoresis microchip with PCB electrodes for contactless conductivity detection

A plug-in electrophoresis microchip for large-scale use aimed at improving maintainability with low fabrication and maintenance costs is proposed in this paper. The plug-in microchip improves the maintainability of a device because the damaged microchannel layer can be changed without needing to cut...

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Detalles Bibliográficos
Autores principales: Yang, Mingpeng, Huang, Zhe, You, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society Publishing 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5990721/
https://www.ncbi.nlm.nih.gov/pubmed/29892366
http://dx.doi.org/10.1098/rsos.171687
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author Yang, Mingpeng
Huang, Zhe
You, Hui
author_facet Yang, Mingpeng
Huang, Zhe
You, Hui
author_sort Yang, Mingpeng
collection PubMed
description A plug-in electrophoresis microchip for large-scale use aimed at improving maintainability with low fabrication and maintenance costs is proposed in this paper. The plug-in microchip improves the maintainability of a device because the damaged microchannel layer can be changed without needing to cut off the circuit wires in the detection component. Obviously, the plug-in structure reduces waste compared with earlier microchips; at present the whole microchip has to be discarded, including the electrode layer and the microchannel layer. The fabrication cost was reduced as far as possible by adopting a steel template and printed circuit board electrodes that avoided the complex photolithography, metal deposition and sputtering processes. The detection performance of our microchip was assessed by electrophoresis experiments. The results showed an acceptable gradient and stable detection performance. The effect of the installation shift between the microchannel layer and the electrode layer brought about by the plug-in structure was also evaluated. The results indicated that, as long as the shift was controlled within a reasonable scope, its effect on the detection performance was acceptable. The plug-in microchip described in this paper represents a new train of thought for the large-scale use and design of portable instruments with electrophoresis microchips in the future.
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spelling pubmed-59907212018-06-11 A plug-in electrophoresis microchip with PCB electrodes for contactless conductivity detection Yang, Mingpeng Huang, Zhe You, Hui R Soc Open Sci Chemistry A plug-in electrophoresis microchip for large-scale use aimed at improving maintainability with low fabrication and maintenance costs is proposed in this paper. The plug-in microchip improves the maintainability of a device because the damaged microchannel layer can be changed without needing to cut off the circuit wires in the detection component. Obviously, the plug-in structure reduces waste compared with earlier microchips; at present the whole microchip has to be discarded, including the electrode layer and the microchannel layer. The fabrication cost was reduced as far as possible by adopting a steel template and printed circuit board electrodes that avoided the complex photolithography, metal deposition and sputtering processes. The detection performance of our microchip was assessed by electrophoresis experiments. The results showed an acceptable gradient and stable detection performance. The effect of the installation shift between the microchannel layer and the electrode layer brought about by the plug-in structure was also evaluated. The results indicated that, as long as the shift was controlled within a reasonable scope, its effect on the detection performance was acceptable. The plug-in microchip described in this paper represents a new train of thought for the large-scale use and design of portable instruments with electrophoresis microchips in the future. The Royal Society Publishing 2018-05-09 /pmc/articles/PMC5990721/ /pubmed/29892366 http://dx.doi.org/10.1098/rsos.171687 Text en © 2018 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
spellingShingle Chemistry
Yang, Mingpeng
Huang, Zhe
You, Hui
A plug-in electrophoresis microchip with PCB electrodes for contactless conductivity detection
title A plug-in electrophoresis microchip with PCB electrodes for contactless conductivity detection
title_full A plug-in electrophoresis microchip with PCB electrodes for contactless conductivity detection
title_fullStr A plug-in electrophoresis microchip with PCB electrodes for contactless conductivity detection
title_full_unstemmed A plug-in electrophoresis microchip with PCB electrodes for contactless conductivity detection
title_short A plug-in electrophoresis microchip with PCB electrodes for contactless conductivity detection
title_sort plug-in electrophoresis microchip with pcb electrodes for contactless conductivity detection
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5990721/
https://www.ncbi.nlm.nih.gov/pubmed/29892366
http://dx.doi.org/10.1098/rsos.171687
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