Cargando…
Reduced graphene oxide-loaded nanocomposite scaffolds for enhancing angiogenesis in tissue engineering applications
Tissue engineering combines cells, scaffolds and signalling molecules to synthesize tissues in vitro. However, the lack of a functioning vascular network severely limits the effective size of a tissue-engineered construct. In this work, we have assessed the potential of reduced graphene oxide (rGO),...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society Publishing
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5990794/ https://www.ncbi.nlm.nih.gov/pubmed/29892387 http://dx.doi.org/10.1098/rsos.172017 |
_version_ | 1783329650408161280 |
---|---|
author | Chakraborty, S. Ponrasu, T. Chandel, S. Dixit, M. Muthuvijayan, V. |
author_facet | Chakraborty, S. Ponrasu, T. Chandel, S. Dixit, M. Muthuvijayan, V. |
author_sort | Chakraborty, S. |
collection | PubMed |
description | Tissue engineering combines cells, scaffolds and signalling molecules to synthesize tissues in vitro. However, the lack of a functioning vascular network severely limits the effective size of a tissue-engineered construct. In this work, we have assessed the potential of reduced graphene oxide (rGO), a non-protein pro-angiogenic moiety, for enhancing angiogenesis in tissue engineering applications. Polyvinyl alcohol/carboxymethyl cellulose (PVA/CMC) scaffolds loaded with different concentrations of rGO nanoparticles were synthesized via lyophilization. Characterization of these scaffolds showed that the rGO-loaded scaffolds retained the thermal and physical properties (swelling, porosity and in vitro biodegradation) of pure PVA/CMC scaffolds. In vitro cytotoxicity studies, using three different cell lines, confirmed that the scaffolds are biocompatible. The scaffolds containing 0.005 and 0.0075% rGO enhanced the proliferation of endothelial cells (EA.hy926) in vitro. In vivo studies using the chick chorioallantoic membrane model showed that the presence of rGO in the PVA/CMC scaffolds significantly enhanced angiogenesis and arteriogenesis. |
format | Online Article Text |
id | pubmed-5990794 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Royal Society Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-59907942018-06-11 Reduced graphene oxide-loaded nanocomposite scaffolds for enhancing angiogenesis in tissue engineering applications Chakraborty, S. Ponrasu, T. Chandel, S. Dixit, M. Muthuvijayan, V. R Soc Open Sci Engineering Tissue engineering combines cells, scaffolds and signalling molecules to synthesize tissues in vitro. However, the lack of a functioning vascular network severely limits the effective size of a tissue-engineered construct. In this work, we have assessed the potential of reduced graphene oxide (rGO), a non-protein pro-angiogenic moiety, for enhancing angiogenesis in tissue engineering applications. Polyvinyl alcohol/carboxymethyl cellulose (PVA/CMC) scaffolds loaded with different concentrations of rGO nanoparticles were synthesized via lyophilization. Characterization of these scaffolds showed that the rGO-loaded scaffolds retained the thermal and physical properties (swelling, porosity and in vitro biodegradation) of pure PVA/CMC scaffolds. In vitro cytotoxicity studies, using three different cell lines, confirmed that the scaffolds are biocompatible. The scaffolds containing 0.005 and 0.0075% rGO enhanced the proliferation of endothelial cells (EA.hy926) in vitro. In vivo studies using the chick chorioallantoic membrane model showed that the presence of rGO in the PVA/CMC scaffolds significantly enhanced angiogenesis and arteriogenesis. The Royal Society Publishing 2018-05-02 /pmc/articles/PMC5990794/ /pubmed/29892387 http://dx.doi.org/10.1098/rsos.172017 Text en © 2018 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Engineering Chakraborty, S. Ponrasu, T. Chandel, S. Dixit, M. Muthuvijayan, V. Reduced graphene oxide-loaded nanocomposite scaffolds for enhancing angiogenesis in tissue engineering applications |
title | Reduced graphene oxide-loaded nanocomposite scaffolds for enhancing angiogenesis in tissue engineering applications |
title_full | Reduced graphene oxide-loaded nanocomposite scaffolds for enhancing angiogenesis in tissue engineering applications |
title_fullStr | Reduced graphene oxide-loaded nanocomposite scaffolds for enhancing angiogenesis in tissue engineering applications |
title_full_unstemmed | Reduced graphene oxide-loaded nanocomposite scaffolds for enhancing angiogenesis in tissue engineering applications |
title_short | Reduced graphene oxide-loaded nanocomposite scaffolds for enhancing angiogenesis in tissue engineering applications |
title_sort | reduced graphene oxide-loaded nanocomposite scaffolds for enhancing angiogenesis in tissue engineering applications |
topic | Engineering |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5990794/ https://www.ncbi.nlm.nih.gov/pubmed/29892387 http://dx.doi.org/10.1098/rsos.172017 |
work_keys_str_mv | AT chakrabortys reducedgrapheneoxideloadednanocompositescaffoldsforenhancingangiogenesisintissueengineeringapplications AT ponrasut reducedgrapheneoxideloadednanocompositescaffoldsforenhancingangiogenesisintissueengineeringapplications AT chandels reducedgrapheneoxideloadednanocompositescaffoldsforenhancingangiogenesisintissueengineeringapplications AT dixitm reducedgrapheneoxideloadednanocompositescaffoldsforenhancingangiogenesisintissueengineeringapplications AT muthuvijayanv reducedgrapheneoxideloadednanocompositescaffoldsforenhancingangiogenesisintissueengineeringapplications |