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Characterization of the “a” determinant region of the hepatitis B virus genome in Iranian patients at different clinical phases of chronic infection

AIM: To determine the distribution of important mutations of the “a” determinant region in the HBV genome among patients in different clinical phases of HBV infection. BACKGROUND: Variations in Hepatitis B infection not only change the outcome of the disease but also the symptoms from which the chro...

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Autores principales: Romani, Sara, Hosseini, Seyed Masoud, Mohebbi, Seyed Reza, Boonstra, Andre, Hosseini Razavi, Armin, Sharifian, Afsaneh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shaheed Beheshti University of Medical Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5990914/
https://www.ncbi.nlm.nih.gov/pubmed/29910854
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author Romani, Sara
Hosseini, Seyed Masoud
Mohebbi, Seyed Reza
Boonstra, Andre
Hosseini Razavi, Armin
Sharifian, Afsaneh
author_facet Romani, Sara
Hosseini, Seyed Masoud
Mohebbi, Seyed Reza
Boonstra, Andre
Hosseini Razavi, Armin
Sharifian, Afsaneh
author_sort Romani, Sara
collection PubMed
description AIM: To determine the distribution of important mutations of the “a” determinant region in the HBV genome among patients in different clinical phases of HBV infection. BACKGROUND: Variations in Hepatitis B infection not only change the outcome of the disease but also the symptoms from which the chronic HBV patients are suffering. METHODS: We have meticulously selected a total of 40 chronic HBV patients from four different subclasses of chronic HBV clinical phases including immune tolerant (IT), immune active (IA), inactive carrier (IC) and hepatitis B e antigen (HBeAg)-negative (ENEG); 10 samples per each phase. Mutations of the “a” determinant region were identified using PCR-Direct sequencing method. RESULTS: 17 amino-acid substitutions at 12 positions inside the “a” determinant were identified in all forty samples; 3 mutations in the IT group, 6 mutations in the IA phase, 3 mutations in the IC patients and 5 mutations in the ENEG phase. Different substitutions were observed in all four clinical phases. The IA phase was the most variant group with the highest number of amino-acid substitutions. CONCLUSION: These results did not reveal a strong pattern to distinguish different clinical phases of Chronic HBV infection, but there are some obvious differences regarding the number and position of mutations between these four clinical phases.
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spelling pubmed-59909142018-06-15 Characterization of the “a” determinant region of the hepatitis B virus genome in Iranian patients at different clinical phases of chronic infection Romani, Sara Hosseini, Seyed Masoud Mohebbi, Seyed Reza Boonstra, Andre Hosseini Razavi, Armin Sharifian, Afsaneh Gastroenterol Hepatol Bed Bench Original Article AIM: To determine the distribution of important mutations of the “a” determinant region in the HBV genome among patients in different clinical phases of HBV infection. BACKGROUND: Variations in Hepatitis B infection not only change the outcome of the disease but also the symptoms from which the chronic HBV patients are suffering. METHODS: We have meticulously selected a total of 40 chronic HBV patients from four different subclasses of chronic HBV clinical phases including immune tolerant (IT), immune active (IA), inactive carrier (IC) and hepatitis B e antigen (HBeAg)-negative (ENEG); 10 samples per each phase. Mutations of the “a” determinant region were identified using PCR-Direct sequencing method. RESULTS: 17 amino-acid substitutions at 12 positions inside the “a” determinant were identified in all forty samples; 3 mutations in the IT group, 6 mutations in the IA phase, 3 mutations in the IC patients and 5 mutations in the ENEG phase. Different substitutions were observed in all four clinical phases. The IA phase was the most variant group with the highest number of amino-acid substitutions. CONCLUSION: These results did not reveal a strong pattern to distinguish different clinical phases of Chronic HBV infection, but there are some obvious differences regarding the number and position of mutations between these four clinical phases. Shaheed Beheshti University of Medical Sciences 2018 /pmc/articles/PMC5990914/ /pubmed/29910854 Text en ©2018 RIGLD, Research Institute for Gastroenterology and Liver Diseases This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Romani, Sara
Hosseini, Seyed Masoud
Mohebbi, Seyed Reza
Boonstra, Andre
Hosseini Razavi, Armin
Sharifian, Afsaneh
Characterization of the “a” determinant region of the hepatitis B virus genome in Iranian patients at different clinical phases of chronic infection
title Characterization of the “a” determinant region of the hepatitis B virus genome in Iranian patients at different clinical phases of chronic infection
title_full Characterization of the “a” determinant region of the hepatitis B virus genome in Iranian patients at different clinical phases of chronic infection
title_fullStr Characterization of the “a” determinant region of the hepatitis B virus genome in Iranian patients at different clinical phases of chronic infection
title_full_unstemmed Characterization of the “a” determinant region of the hepatitis B virus genome in Iranian patients at different clinical phases of chronic infection
title_short Characterization of the “a” determinant region of the hepatitis B virus genome in Iranian patients at different clinical phases of chronic infection
title_sort characterization of the “a” determinant region of the hepatitis b virus genome in iranian patients at different clinical phases of chronic infection
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5990914/
https://www.ncbi.nlm.nih.gov/pubmed/29910854
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