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Assessment of potential biomarkers of atherosclerosis in Indian patients with type 2 diabetes mellitus
BACKGROUND & OBJECTIVES: Various biological markers of subclinical atherosclerosis have been proposed to predict cardiovascular events in patients with diabetes mellitus (DM). However, there are only a few clinical studies assessing the role of invasive biomarkers [CD-36, peroxisome proliferator...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5991114/ https://www.ncbi.nlm.nih.gov/pubmed/29806605 http://dx.doi.org/10.4103/ijmr.IJMR_852_16 |
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author | Kulkarni, Namrata Bindurao Ganu, Meghana Ulhas Godbole, Sanjay Ganpati Deo, Sudha S. |
author_facet | Kulkarni, Namrata Bindurao Ganu, Meghana Ulhas Godbole, Sanjay Ganpati Deo, Sudha S. |
author_sort | Kulkarni, Namrata Bindurao |
collection | PubMed |
description | BACKGROUND & OBJECTIVES: Various biological markers of subclinical atherosclerosis have been proposed to predict cardiovascular events in patients with diabetes mellitus (DM). However, there are only a few clinical studies assessing the role of invasive biomarkers [CD-36, peroxisome proliferator-activated receptor gamma (PPAR-γ) and YKL-40] in Indian patients with type 2 DM (T2DM). Hence, the present study was conducted to assess protein levels and gene expression of CD-36, PPAR-γ and YKL-40 in patients with T2DM and compare that with hypertensive and healthy controls. METHODS: All the participants were subjected to medical history, anthropometric measurements and biochemical and biomarker (ELISA and real-time polymerase chain reaction) estimations. The study groups consisted of patients with T2DM (>5 yr) with hypertension (n=55), patients with T2DM (<2 yr) without hypertension (n=28), hypertensive controls (n=31) and healthy controls (n=30). RESULTS: Gene expressions of YKL-40 and CD36 were significantly higher in patients with T2DM (>5 yr) with hypertension compared to healthy controls (P=0.006). In addition, a significant increase in serum levels of sCD36, PPAR-γ and YKL-40 was observed in patients with T2DM (>5 yr) with hypertension compared to healthy controls (P< 0.05). Serum levels as well as gene expression of CD36 showed significant correlation with serum levels as well as gene expression of PPAR-γ (ρ=0.45 and ρ=0.51; P< 0.001), respectively. INTERPRETATION & CONCLUSIONS: CD36 and YKL-40 may be potential inflammatory biomarkers for early onset of atherosclerosis in patients with T2DM. |
format | Online Article Text |
id | pubmed-5991114 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59911142018-06-21 Assessment of potential biomarkers of atherosclerosis in Indian patients with type 2 diabetes mellitus Kulkarni, Namrata Bindurao Ganu, Meghana Ulhas Godbole, Sanjay Ganpati Deo, Sudha S. Indian J Med Res Original Article BACKGROUND & OBJECTIVES: Various biological markers of subclinical atherosclerosis have been proposed to predict cardiovascular events in patients with diabetes mellitus (DM). However, there are only a few clinical studies assessing the role of invasive biomarkers [CD-36, peroxisome proliferator-activated receptor gamma (PPAR-γ) and YKL-40] in Indian patients with type 2 DM (T2DM). Hence, the present study was conducted to assess protein levels and gene expression of CD-36, PPAR-γ and YKL-40 in patients with T2DM and compare that with hypertensive and healthy controls. METHODS: All the participants were subjected to medical history, anthropometric measurements and biochemical and biomarker (ELISA and real-time polymerase chain reaction) estimations. The study groups consisted of patients with T2DM (>5 yr) with hypertension (n=55), patients with T2DM (<2 yr) without hypertension (n=28), hypertensive controls (n=31) and healthy controls (n=30). RESULTS: Gene expressions of YKL-40 and CD36 were significantly higher in patients with T2DM (>5 yr) with hypertension compared to healthy controls (P=0.006). In addition, a significant increase in serum levels of sCD36, PPAR-γ and YKL-40 was observed in patients with T2DM (>5 yr) with hypertension compared to healthy controls (P< 0.05). Serum levels as well as gene expression of CD36 showed significant correlation with serum levels as well as gene expression of PPAR-γ (ρ=0.45 and ρ=0.51; P< 0.001), respectively. INTERPRETATION & CONCLUSIONS: CD36 and YKL-40 may be potential inflammatory biomarkers for early onset of atherosclerosis in patients with T2DM. Medknow Publications & Media Pvt Ltd 2018-02 /pmc/articles/PMC5991114/ /pubmed/29806605 http://dx.doi.org/10.4103/ijmr.IJMR_852_16 Text en Copyright: © 2018 Indian Journal of Medical Research http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Kulkarni, Namrata Bindurao Ganu, Meghana Ulhas Godbole, Sanjay Ganpati Deo, Sudha S. Assessment of potential biomarkers of atherosclerosis in Indian patients with type 2 diabetes mellitus |
title | Assessment of potential biomarkers of atherosclerosis in Indian patients with type 2 diabetes mellitus |
title_full | Assessment of potential biomarkers of atherosclerosis in Indian patients with type 2 diabetes mellitus |
title_fullStr | Assessment of potential biomarkers of atherosclerosis in Indian patients with type 2 diabetes mellitus |
title_full_unstemmed | Assessment of potential biomarkers of atherosclerosis in Indian patients with type 2 diabetes mellitus |
title_short | Assessment of potential biomarkers of atherosclerosis in Indian patients with type 2 diabetes mellitus |
title_sort | assessment of potential biomarkers of atherosclerosis in indian patients with type 2 diabetes mellitus |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5991114/ https://www.ncbi.nlm.nih.gov/pubmed/29806605 http://dx.doi.org/10.4103/ijmr.IJMR_852_16 |
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