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Assessment of potential biomarkers of atherosclerosis in Indian patients with type 2 diabetes mellitus

BACKGROUND & OBJECTIVES: Various biological markers of subclinical atherosclerosis have been proposed to predict cardiovascular events in patients with diabetes mellitus (DM). However, there are only a few clinical studies assessing the role of invasive biomarkers [CD-36, peroxisome proliferator...

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Autores principales: Kulkarni, Namrata Bindurao, Ganu, Meghana Ulhas, Godbole, Sanjay Ganpati, Deo, Sudha S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5991114/
https://www.ncbi.nlm.nih.gov/pubmed/29806605
http://dx.doi.org/10.4103/ijmr.IJMR_852_16
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author Kulkarni, Namrata Bindurao
Ganu, Meghana Ulhas
Godbole, Sanjay Ganpati
Deo, Sudha S.
author_facet Kulkarni, Namrata Bindurao
Ganu, Meghana Ulhas
Godbole, Sanjay Ganpati
Deo, Sudha S.
author_sort Kulkarni, Namrata Bindurao
collection PubMed
description BACKGROUND & OBJECTIVES: Various biological markers of subclinical atherosclerosis have been proposed to predict cardiovascular events in patients with diabetes mellitus (DM). However, there are only a few clinical studies assessing the role of invasive biomarkers [CD-36, peroxisome proliferator-activated receptor gamma (PPAR-γ) and YKL-40] in Indian patients with type 2 DM (T2DM). Hence, the present study was conducted to assess protein levels and gene expression of CD-36, PPAR-γ and YKL-40 in patients with T2DM and compare that with hypertensive and healthy controls. METHODS: All the participants were subjected to medical history, anthropometric measurements and biochemical and biomarker (ELISA and real-time polymerase chain reaction) estimations. The study groups consisted of patients with T2DM (>5 yr) with hypertension (n=55), patients with T2DM (<2 yr) without hypertension (n=28), hypertensive controls (n=31) and healthy controls (n=30). RESULTS: Gene expressions of YKL-40 and CD36 were significantly higher in patients with T2DM (>5 yr) with hypertension compared to healthy controls (P=0.006). In addition, a significant increase in serum levels of sCD36, PPAR-γ and YKL-40 was observed in patients with T2DM (>5 yr) with hypertension compared to healthy controls (P< 0.05). Serum levels as well as gene expression of CD36 showed significant correlation with serum levels as well as gene expression of PPAR-γ (ρ=0.45 and ρ=0.51; P< 0.001), respectively. INTERPRETATION & CONCLUSIONS: CD36 and YKL-40 may be potential inflammatory biomarkers for early onset of atherosclerosis in patients with T2DM.
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spelling pubmed-59911142018-06-21 Assessment of potential biomarkers of atherosclerosis in Indian patients with type 2 diabetes mellitus Kulkarni, Namrata Bindurao Ganu, Meghana Ulhas Godbole, Sanjay Ganpati Deo, Sudha S. Indian J Med Res Original Article BACKGROUND & OBJECTIVES: Various biological markers of subclinical atherosclerosis have been proposed to predict cardiovascular events in patients with diabetes mellitus (DM). However, there are only a few clinical studies assessing the role of invasive biomarkers [CD-36, peroxisome proliferator-activated receptor gamma (PPAR-γ) and YKL-40] in Indian patients with type 2 DM (T2DM). Hence, the present study was conducted to assess protein levels and gene expression of CD-36, PPAR-γ and YKL-40 in patients with T2DM and compare that with hypertensive and healthy controls. METHODS: All the participants were subjected to medical history, anthropometric measurements and biochemical and biomarker (ELISA and real-time polymerase chain reaction) estimations. The study groups consisted of patients with T2DM (>5 yr) with hypertension (n=55), patients with T2DM (<2 yr) without hypertension (n=28), hypertensive controls (n=31) and healthy controls (n=30). RESULTS: Gene expressions of YKL-40 and CD36 were significantly higher in patients with T2DM (>5 yr) with hypertension compared to healthy controls (P=0.006). In addition, a significant increase in serum levels of sCD36, PPAR-γ and YKL-40 was observed in patients with T2DM (>5 yr) with hypertension compared to healthy controls (P< 0.05). Serum levels as well as gene expression of CD36 showed significant correlation with serum levels as well as gene expression of PPAR-γ (ρ=0.45 and ρ=0.51; P< 0.001), respectively. INTERPRETATION & CONCLUSIONS: CD36 and YKL-40 may be potential inflammatory biomarkers for early onset of atherosclerosis in patients with T2DM. Medknow Publications & Media Pvt Ltd 2018-02 /pmc/articles/PMC5991114/ /pubmed/29806605 http://dx.doi.org/10.4103/ijmr.IJMR_852_16 Text en Copyright: © 2018 Indian Journal of Medical Research http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Kulkarni, Namrata Bindurao
Ganu, Meghana Ulhas
Godbole, Sanjay Ganpati
Deo, Sudha S.
Assessment of potential biomarkers of atherosclerosis in Indian patients with type 2 diabetes mellitus
title Assessment of potential biomarkers of atherosclerosis in Indian patients with type 2 diabetes mellitus
title_full Assessment of potential biomarkers of atherosclerosis in Indian patients with type 2 diabetes mellitus
title_fullStr Assessment of potential biomarkers of atherosclerosis in Indian patients with type 2 diabetes mellitus
title_full_unstemmed Assessment of potential biomarkers of atherosclerosis in Indian patients with type 2 diabetes mellitus
title_short Assessment of potential biomarkers of atherosclerosis in Indian patients with type 2 diabetes mellitus
title_sort assessment of potential biomarkers of atherosclerosis in indian patients with type 2 diabetes mellitus
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5991114/
https://www.ncbi.nlm.nih.gov/pubmed/29806605
http://dx.doi.org/10.4103/ijmr.IJMR_852_16
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