Cargando…

Evaluation of genetic relationship between 15 bamboo species of North-East India based on ISSR marker analysis

The classification of bamboos based on floral morphology and reproductive characters is very hard due to erratic flowering behavior and unusually long reproductive cycle. The application of reliable and effective DNA molecular markers is highly essential to address this problem. In the present inves...

Descripción completa

Detalles Bibliográficos
Autores principales: Amom, Thoungamba, Tikendra, Leimapokpam, Rahaman, Hamidur, Potshangbam, Angamba, Nongdam, Potshangbam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shiraz University 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5991527/
https://www.ncbi.nlm.nih.gov/pubmed/29911118
http://dx.doi.org/10.22099/mbrc.2018.28378.1303
_version_ 1783329844195491840
author Amom, Thoungamba
Tikendra, Leimapokpam
Rahaman, Hamidur
Potshangbam, Angamba
Nongdam, Potshangbam
author_facet Amom, Thoungamba
Tikendra, Leimapokpam
Rahaman, Hamidur
Potshangbam, Angamba
Nongdam, Potshangbam
author_sort Amom, Thoungamba
collection PubMed
description The classification of bamboos based on floral morphology and reproductive characters is very hard due to erratic flowering behavior and unusually long reproductive cycle. The application of reliable and effective DNA molecular markers is highly essential to address this problem. In the present investigation, inter-simple sequence repeats (ISSR) markers were employed to study phylogenetic relationship of 15 different bamboos of North-East India. The 10 ISSR primers generated 116 polymorphic loci or scorable bands with average of 11.6 bands per primer. The genetic similarity coefficient ranged from 0.41-0.76 showing high genetic polymorphism among different bamboo species. The phylogenetic tree constructed based on genetic similarity matrix revealed genetic proximity of endemic Bambusa mizorameana to other five Dendrocalamus species by clustering into a single group, while Dendrocalamus manipureanus segregated from the cluster indicating its genetic divergent character. Except for Schizotachyum fuchsianum, the three Schizotachyum species viz., S. dullooa, S. pergracile and S. munroi exhibited close genetic affinity by grouping into a single minor clade. Principal coordinates analysis (PCoA) showed distribution of different bamboos species in the plot in accordance to unweighted pair-group method with arithmetic average (UPGMA) cluster analysis. Genetic relationship of 15 different bamboos as revealed from the dendrogram and PCoA analysis reasonably conformed to traditional system of classification though slight disagreement existed. This is the first report on the successful use of ISSR markers in the phylogenetic and genetic variation studies of 15 important bamboos of the region including the endemic bamboo species of B. mizorameana, B. manipureana, D. sikkimensis and D. manipureanus.
format Online
Article
Text
id pubmed-5991527
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Shiraz University
record_format MEDLINE/PubMed
spelling pubmed-59915272018-06-15 Evaluation of genetic relationship between 15 bamboo species of North-East India based on ISSR marker analysis Amom, Thoungamba Tikendra, Leimapokpam Rahaman, Hamidur Potshangbam, Angamba Nongdam, Potshangbam Mol Biol Res Commun Original Article The classification of bamboos based on floral morphology and reproductive characters is very hard due to erratic flowering behavior and unusually long reproductive cycle. The application of reliable and effective DNA molecular markers is highly essential to address this problem. In the present investigation, inter-simple sequence repeats (ISSR) markers were employed to study phylogenetic relationship of 15 different bamboos of North-East India. The 10 ISSR primers generated 116 polymorphic loci or scorable bands with average of 11.6 bands per primer. The genetic similarity coefficient ranged from 0.41-0.76 showing high genetic polymorphism among different bamboo species. The phylogenetic tree constructed based on genetic similarity matrix revealed genetic proximity of endemic Bambusa mizorameana to other five Dendrocalamus species by clustering into a single group, while Dendrocalamus manipureanus segregated from the cluster indicating its genetic divergent character. Except for Schizotachyum fuchsianum, the three Schizotachyum species viz., S. dullooa, S. pergracile and S. munroi exhibited close genetic affinity by grouping into a single minor clade. Principal coordinates analysis (PCoA) showed distribution of different bamboos species in the plot in accordance to unweighted pair-group method with arithmetic average (UPGMA) cluster analysis. Genetic relationship of 15 different bamboos as revealed from the dendrogram and PCoA analysis reasonably conformed to traditional system of classification though slight disagreement existed. This is the first report on the successful use of ISSR markers in the phylogenetic and genetic variation studies of 15 important bamboos of the region including the endemic bamboo species of B. mizorameana, B. manipureana, D. sikkimensis and D. manipureanus. Shiraz University 2018-03 /pmc/articles/PMC5991527/ /pubmed/29911118 http://dx.doi.org/10.22099/mbrc.2018.28378.1303 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Amom, Thoungamba
Tikendra, Leimapokpam
Rahaman, Hamidur
Potshangbam, Angamba
Nongdam, Potshangbam
Evaluation of genetic relationship between 15 bamboo species of North-East India based on ISSR marker analysis
title Evaluation of genetic relationship between 15 bamboo species of North-East India based on ISSR marker analysis
title_full Evaluation of genetic relationship between 15 bamboo species of North-East India based on ISSR marker analysis
title_fullStr Evaluation of genetic relationship between 15 bamboo species of North-East India based on ISSR marker analysis
title_full_unstemmed Evaluation of genetic relationship between 15 bamboo species of North-East India based on ISSR marker analysis
title_short Evaluation of genetic relationship between 15 bamboo species of North-East India based on ISSR marker analysis
title_sort evaluation of genetic relationship between 15 bamboo species of north-east india based on issr marker analysis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5991527/
https://www.ncbi.nlm.nih.gov/pubmed/29911118
http://dx.doi.org/10.22099/mbrc.2018.28378.1303
work_keys_str_mv AT amomthoungamba evaluationofgeneticrelationshipbetween15bamboospeciesofnortheastindiabasedonissrmarkeranalysis
AT tikendraleimapokpam evaluationofgeneticrelationshipbetween15bamboospeciesofnortheastindiabasedonissrmarkeranalysis
AT rahamanhamidur evaluationofgeneticrelationshipbetween15bamboospeciesofnortheastindiabasedonissrmarkeranalysis
AT potshangbamangamba evaluationofgeneticrelationshipbetween15bamboospeciesofnortheastindiabasedonissrmarkeranalysis
AT nongdampotshangbam evaluationofgeneticrelationshipbetween15bamboospeciesofnortheastindiabasedonissrmarkeranalysis