Cargando…

Evaluation of prodynorphin gene polymorphisms and their association with heroin addiction in a sample of the southeast Iranian population

Genetic factors are supposed to account for about 30-50% of the predisposition to cocaine and heroin addiction. This study aims at investigating the effect of rs2281285, rs2235749, rs910080 and 68bp VNTR polymorphisms of prodynorphin (PDYN) gene on heroin dependence risk in a sample of the southeast...

Descripción completa

Detalles Bibliográficos
Autores principales: Hashemi, Mohammad, Shakiba, Mansour, Sanaei, Sara, Shahkar, Ghazaleh, Rezaei, Maryam, Mojahed, Azizolla, Bahari, Gholamreza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shiraz University 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5991531/
https://www.ncbi.nlm.nih.gov/pubmed/29911117
http://dx.doi.org/10.22099/mbrc.2017.27182.1294
_version_ 1783329845471608832
author Hashemi, Mohammad
Shakiba, Mansour
Sanaei, Sara
Shahkar, Ghazaleh
Rezaei, Maryam
Mojahed, Azizolla
Bahari, Gholamreza
author_facet Hashemi, Mohammad
Shakiba, Mansour
Sanaei, Sara
Shahkar, Ghazaleh
Rezaei, Maryam
Mojahed, Azizolla
Bahari, Gholamreza
author_sort Hashemi, Mohammad
collection PubMed
description Genetic factors are supposed to account for about 30-50% of the predisposition to cocaine and heroin addiction. This study aims at investigating the effect of rs2281285, rs2235749, rs910080 and 68bp VNTR polymorphisms of prodynorphin (PDYN) gene on heroin dependence risk in a sample of the southeast Iranian population. This case-control study was done on 216 heroin dependence subjects and 219 healthy subjects. Genomic DNA was extracted from peripheral blood cells using salting out method. Genotyping of PDYN polymorphisms were performed using polymerase chain reaction (PCR) or PCR-RFLP method. The findings showed that PDYN rs910080 T>C variant significantly increased the risk of heroin dependence (OR=7.91, 95%CI=3.36-18.61, P<0.0001, CC vs TT; OR=7.53, 95%CI=3.30-17.16, P<0.0001, CC vs TT+TC; OR=1.75, 95%CI=1.33-2.32, p<0.0001, C vs T). The rs2235749 C>T, rs2281285 A>G and 68bp VNTR variants of PDYN gene were not associated with heroin dependence. Altogether, our results provide an association between rs910080 polymorphism of PDYN gene and risk of heroin dependence in a sample of the southeast Iranian population.
format Online
Article
Text
id pubmed-5991531
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Shiraz University
record_format MEDLINE/PubMed
spelling pubmed-59915312018-06-15 Evaluation of prodynorphin gene polymorphisms and their association with heroin addiction in a sample of the southeast Iranian population Hashemi, Mohammad Shakiba, Mansour Sanaei, Sara Shahkar, Ghazaleh Rezaei, Maryam Mojahed, Azizolla Bahari, Gholamreza Mol Biol Res Commun Original Article Genetic factors are supposed to account for about 30-50% of the predisposition to cocaine and heroin addiction. This study aims at investigating the effect of rs2281285, rs2235749, rs910080 and 68bp VNTR polymorphisms of prodynorphin (PDYN) gene on heroin dependence risk in a sample of the southeast Iranian population. This case-control study was done on 216 heroin dependence subjects and 219 healthy subjects. Genomic DNA was extracted from peripheral blood cells using salting out method. Genotyping of PDYN polymorphisms were performed using polymerase chain reaction (PCR) or PCR-RFLP method. The findings showed that PDYN rs910080 T>C variant significantly increased the risk of heroin dependence (OR=7.91, 95%CI=3.36-18.61, P<0.0001, CC vs TT; OR=7.53, 95%CI=3.30-17.16, P<0.0001, CC vs TT+TC; OR=1.75, 95%CI=1.33-2.32, p<0.0001, C vs T). The rs2235749 C>T, rs2281285 A>G and 68bp VNTR variants of PDYN gene were not associated with heroin dependence. Altogether, our results provide an association between rs910080 polymorphism of PDYN gene and risk of heroin dependence in a sample of the southeast Iranian population. Shiraz University 2018-03 /pmc/articles/PMC5991531/ /pubmed/29911117 http://dx.doi.org/10.22099/mbrc.2017.27182.1294 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Hashemi, Mohammad
Shakiba, Mansour
Sanaei, Sara
Shahkar, Ghazaleh
Rezaei, Maryam
Mojahed, Azizolla
Bahari, Gholamreza
Evaluation of prodynorphin gene polymorphisms and their association with heroin addiction in a sample of the southeast Iranian population
title Evaluation of prodynorphin gene polymorphisms and their association with heroin addiction in a sample of the southeast Iranian population
title_full Evaluation of prodynorphin gene polymorphisms and their association with heroin addiction in a sample of the southeast Iranian population
title_fullStr Evaluation of prodynorphin gene polymorphisms and their association with heroin addiction in a sample of the southeast Iranian population
title_full_unstemmed Evaluation of prodynorphin gene polymorphisms and their association with heroin addiction in a sample of the southeast Iranian population
title_short Evaluation of prodynorphin gene polymorphisms and their association with heroin addiction in a sample of the southeast Iranian population
title_sort evaluation of prodynorphin gene polymorphisms and their association with heroin addiction in a sample of the southeast iranian population
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5991531/
https://www.ncbi.nlm.nih.gov/pubmed/29911117
http://dx.doi.org/10.22099/mbrc.2017.27182.1294
work_keys_str_mv AT hashemimohammad evaluationofprodynorphingenepolymorphismsandtheirassociationwithheroinaddictioninasampleofthesoutheastiranianpopulation
AT shakibamansour evaluationofprodynorphingenepolymorphismsandtheirassociationwithheroinaddictioninasampleofthesoutheastiranianpopulation
AT sanaeisara evaluationofprodynorphingenepolymorphismsandtheirassociationwithheroinaddictioninasampleofthesoutheastiranianpopulation
AT shahkarghazaleh evaluationofprodynorphingenepolymorphismsandtheirassociationwithheroinaddictioninasampleofthesoutheastiranianpopulation
AT rezaeimaryam evaluationofprodynorphingenepolymorphismsandtheirassociationwithheroinaddictioninasampleofthesoutheastiranianpopulation
AT mojahedazizolla evaluationofprodynorphingenepolymorphismsandtheirassociationwithheroinaddictioninasampleofthesoutheastiranianpopulation
AT baharigholamreza evaluationofprodynorphingenepolymorphismsandtheirassociationwithheroinaddictioninasampleofthesoutheastiranianpopulation