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Wheat differential gene expression induced by different races of Puccinia triticina
Puccinia triticina, the causal agent of wheat leaf rust, causes significant losses in wheat yield and quality each year worldwide. During leaf rust infection, the host plant recognizes numerous molecules, some of which trigger host defenses. Although P. triticina reproduces clonally, there is still...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5991701/ https://www.ncbi.nlm.nih.gov/pubmed/29879135 http://dx.doi.org/10.1371/journal.pone.0198350 |
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author | Neugebauer, Kerri A. Bruce, Myron Todd, Tim Trick, Harold N. Fellers, John P. |
author_facet | Neugebauer, Kerri A. Bruce, Myron Todd, Tim Trick, Harold N. Fellers, John P. |
author_sort | Neugebauer, Kerri A. |
collection | PubMed |
description | Puccinia triticina, the causal agent of wheat leaf rust, causes significant losses in wheat yield and quality each year worldwide. During leaf rust infection, the host plant recognizes numerous molecules, some of which trigger host defenses. Although P. triticina reproduces clonally, there is still variation within the population due to a high mutation frequency, host specificity, and environmental adaptation. This study explores how wheat responds on a gene expression level to different P. triticina races. Six P. triticina races were inoculated onto a susceptible wheat variety and samples were taken at six days post inoculation, just prior to pustule eruption. RNA sequence data identified 63 wheat genes differentially expressed between the six races. A time course, conducted over the first seven days post inoculation, was used to examine the expression pattern of 63 genes during infection. Forty-seven wheat genes were verified to have differential expression. Three common expression patterns were identified. In addition, two genes were associated with race specific gene expression. Differential expression of an ER molecular chaperone gene was associated with races from two different P. triticina lineages. Also, differential expression in an alanine glyoxylate aminotransferase gene was associated with races with virulence shifts for leaf rust resistance genes. |
format | Online Article Text |
id | pubmed-5991701 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-59917012018-06-16 Wheat differential gene expression induced by different races of Puccinia triticina Neugebauer, Kerri A. Bruce, Myron Todd, Tim Trick, Harold N. Fellers, John P. PLoS One Research Article Puccinia triticina, the causal agent of wheat leaf rust, causes significant losses in wheat yield and quality each year worldwide. During leaf rust infection, the host plant recognizes numerous molecules, some of which trigger host defenses. Although P. triticina reproduces clonally, there is still variation within the population due to a high mutation frequency, host specificity, and environmental adaptation. This study explores how wheat responds on a gene expression level to different P. triticina races. Six P. triticina races were inoculated onto a susceptible wheat variety and samples were taken at six days post inoculation, just prior to pustule eruption. RNA sequence data identified 63 wheat genes differentially expressed between the six races. A time course, conducted over the first seven days post inoculation, was used to examine the expression pattern of 63 genes during infection. Forty-seven wheat genes were verified to have differential expression. Three common expression patterns were identified. In addition, two genes were associated with race specific gene expression. Differential expression of an ER molecular chaperone gene was associated with races from two different P. triticina lineages. Also, differential expression in an alanine glyoxylate aminotransferase gene was associated with races with virulence shifts for leaf rust resistance genes. Public Library of Science 2018-06-07 /pmc/articles/PMC5991701/ /pubmed/29879135 http://dx.doi.org/10.1371/journal.pone.0198350 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Neugebauer, Kerri A. Bruce, Myron Todd, Tim Trick, Harold N. Fellers, John P. Wheat differential gene expression induced by different races of Puccinia triticina |
title | Wheat differential gene expression induced by different races of Puccinia triticina |
title_full | Wheat differential gene expression induced by different races of Puccinia triticina |
title_fullStr | Wheat differential gene expression induced by different races of Puccinia triticina |
title_full_unstemmed | Wheat differential gene expression induced by different races of Puccinia triticina |
title_short | Wheat differential gene expression induced by different races of Puccinia triticina |
title_sort | wheat differential gene expression induced by different races of puccinia triticina |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5991701/ https://www.ncbi.nlm.nih.gov/pubmed/29879135 http://dx.doi.org/10.1371/journal.pone.0198350 |
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