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Systematic metabolic profiling and bioactivity assays for bioconversion of Aceraceae family

Plants are an important and inexhaustible source of bioactive molecules in food, medicine, agriculture, and industry. In this study, we performed systematic liquid chromatography–mass spectrometry (LC-MS)-based metabolic profiling coupled with antioxidant assays for indigenous plant family extracts....

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Autores principales: Park, Jinyong, Suh, Dong Ho, Singh, Digar, Lee, Sarah, Lee, Jong Seok, Lee, Choong Hwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5991731/
https://www.ncbi.nlm.nih.gov/pubmed/29879203
http://dx.doi.org/10.1371/journal.pone.0198739
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author Park, Jinyong
Suh, Dong Ho
Singh, Digar
Lee, Sarah
Lee, Jong Seok
Lee, Choong Hwan
author_facet Park, Jinyong
Suh, Dong Ho
Singh, Digar
Lee, Sarah
Lee, Jong Seok
Lee, Choong Hwan
author_sort Park, Jinyong
collection PubMed
description Plants are an important and inexhaustible source of bioactive molecules in food, medicine, agriculture, and industry. In this study, we performed systematic liquid chromatography–mass spectrometry (LC-MS)-based metabolic profiling coupled with antioxidant assays for indigenous plant family extracts. Partial least-squares discriminant analysis of LC-MS datasets for the extracts of 34 plant species belonging to the families Aceraceae, Asteraceae, and Rosaceae showed that these species were clustered according to their respective phylogenies. In particular, seven Aceraceae species were clearly demarcated with higher average antioxidant activities, rationalizing their application for bioconversion studies. On the basis of further evaluation of the interspecies variability of metabolic profiles and antioxidant activities among Aceraceae family plants, we found that Acer tataricum (TA) extracts were clearly distinguished from those of other species, with a higher relative abundance of tannin derivatives. Further, we detected a strong positive correlation between most tannin derivatives and the observed higher antioxidant activities. Following Aspergillus oryzae-mediated fermentative bioconversion of Acer plant extracts, we observed a time-correlated (0–8 days) linear increase in antioxidant phenotypes for all species, with TA having the highest activity. Temporal analysis of the MS data revealed tannin bioconversion mechanisms with a relatively higher abundance of gallic acid (m/z 169) accumulated at the end of 8 days, particularly in TA. Similarly, quercetin precursor (glycoside) metabolites were also transformed to quercetin aglycones (m/z 301) in most Acer plant extracts. The present study underscores the efficacy of fermentative bioconversion strategies aimed at enhancing the quality and availability of bioactive metabolites from plant extracts.
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spelling pubmed-59917312018-06-16 Systematic metabolic profiling and bioactivity assays for bioconversion of Aceraceae family Park, Jinyong Suh, Dong Ho Singh, Digar Lee, Sarah Lee, Jong Seok Lee, Choong Hwan PLoS One Research Article Plants are an important and inexhaustible source of bioactive molecules in food, medicine, agriculture, and industry. In this study, we performed systematic liquid chromatography–mass spectrometry (LC-MS)-based metabolic profiling coupled with antioxidant assays for indigenous plant family extracts. Partial least-squares discriminant analysis of LC-MS datasets for the extracts of 34 plant species belonging to the families Aceraceae, Asteraceae, and Rosaceae showed that these species were clustered according to their respective phylogenies. In particular, seven Aceraceae species were clearly demarcated with higher average antioxidant activities, rationalizing their application for bioconversion studies. On the basis of further evaluation of the interspecies variability of metabolic profiles and antioxidant activities among Aceraceae family plants, we found that Acer tataricum (TA) extracts were clearly distinguished from those of other species, with a higher relative abundance of tannin derivatives. Further, we detected a strong positive correlation between most tannin derivatives and the observed higher antioxidant activities. Following Aspergillus oryzae-mediated fermentative bioconversion of Acer plant extracts, we observed a time-correlated (0–8 days) linear increase in antioxidant phenotypes for all species, with TA having the highest activity. Temporal analysis of the MS data revealed tannin bioconversion mechanisms with a relatively higher abundance of gallic acid (m/z 169) accumulated at the end of 8 days, particularly in TA. Similarly, quercetin precursor (glycoside) metabolites were also transformed to quercetin aglycones (m/z 301) in most Acer plant extracts. The present study underscores the efficacy of fermentative bioconversion strategies aimed at enhancing the quality and availability of bioactive metabolites from plant extracts. Public Library of Science 2018-06-07 /pmc/articles/PMC5991731/ /pubmed/29879203 http://dx.doi.org/10.1371/journal.pone.0198739 Text en © 2018 Park et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Park, Jinyong
Suh, Dong Ho
Singh, Digar
Lee, Sarah
Lee, Jong Seok
Lee, Choong Hwan
Systematic metabolic profiling and bioactivity assays for bioconversion of Aceraceae family
title Systematic metabolic profiling and bioactivity assays for bioconversion of Aceraceae family
title_full Systematic metabolic profiling and bioactivity assays for bioconversion of Aceraceae family
title_fullStr Systematic metabolic profiling and bioactivity assays for bioconversion of Aceraceae family
title_full_unstemmed Systematic metabolic profiling and bioactivity assays for bioconversion of Aceraceae family
title_short Systematic metabolic profiling and bioactivity assays for bioconversion of Aceraceae family
title_sort systematic metabolic profiling and bioactivity assays for bioconversion of aceraceae family
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5991731/
https://www.ncbi.nlm.nih.gov/pubmed/29879203
http://dx.doi.org/10.1371/journal.pone.0198739
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