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MyD88 Deficiency Protects Against Dry Eye–Induced Damage
PURPOSE: Dry eye disease (DED) is a multifactorial disease associated with ocular surface inflammation. Toll-like receptors (TLRs) are integral in the initiation of inflammatory signaling. Therefore, we evaluated the effect of TLR-deficiency on dry eye–related ocular surface damage and inflammation...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Association for Research in Vision and Ophthalmology
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5991808/ https://www.ncbi.nlm.nih.gov/pubmed/30025110 http://dx.doi.org/10.1167/iovs.17-23397 |
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author | Reins, Rose Y. Lema, Carolina Courson, Justin Kunnen, Carolina M. E. Redfern, Rachel L. |
author_facet | Reins, Rose Y. Lema, Carolina Courson, Justin Kunnen, Carolina M. E. Redfern, Rachel L. |
author_sort | Reins, Rose Y. |
collection | PubMed |
description | PURPOSE: Dry eye disease (DED) is a multifactorial disease associated with ocular surface inflammation. Toll-like receptors (TLRs) are integral in the initiation of inflammatory signaling. Therefore, we evaluated the effect of TLR-deficiency on dry eye–related ocular surface damage and inflammation using a mouse model of experimental dry eye (EDE). METHODS: C57BL/6 wild-type (WT), MyD88(−/−), and IL-1R(−/−) mice were exposed to EDE conditions for 5 days. Tear production was measured by phenol red thread test and ocular surface damage assessed with fluorescein staining. Corneal homogenates were obtained for matrix metalloproteinase (MMP) and cytokine expression analysis by Luminex assay and quantitative PCR. In addition, whole eyes and eyelids were dissected and goblet cells and Meibomian glands were imaged, respectively. RESULTS: Following 5 days of EDE, WT mice had extensive ocular surface staining, while MyD88(−/−) mice had no increased staining above non-EDE conditions. Similarly, MyD88(−/−) mice did not have increased corneal MMP-2, 3, or 8 concentrations, as seen with WT mice. MyD88-deficiency also resulted in decreased corneal cytokine levels. In addition, MyD88(−/−) mice had significantly lower conjunctival goblet cell counts compared with both WT (EDE) and IL-1R(−/−) (non-EDE) mice. However, there was no difference in Meibomian gland morphology between WT, IL-1R(−/−), and MyD88(−/−) mice. CONCLUSIONS: These studies demonstrate the importance of TLR signaling in dry eye development. Mice lacking TLR signaling, MyD88(−/−), were protected from EDE-induced ocular surface damage and inflammatory mediator expression, warranting further investigation into TLR inhibition as a potential therapeutic for DED. |
format | Online Article Text |
id | pubmed-5991808 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Association for Research in Vision and Ophthalmology |
record_format | MEDLINE/PubMed |
spelling | pubmed-59918082018-06-08 MyD88 Deficiency Protects Against Dry Eye–Induced Damage Reins, Rose Y. Lema, Carolina Courson, Justin Kunnen, Carolina M. E. Redfern, Rachel L. Invest Ophthalmol Vis Sci Immunology and Microbiology PURPOSE: Dry eye disease (DED) is a multifactorial disease associated with ocular surface inflammation. Toll-like receptors (TLRs) are integral in the initiation of inflammatory signaling. Therefore, we evaluated the effect of TLR-deficiency on dry eye–related ocular surface damage and inflammation using a mouse model of experimental dry eye (EDE). METHODS: C57BL/6 wild-type (WT), MyD88(−/−), and IL-1R(−/−) mice were exposed to EDE conditions for 5 days. Tear production was measured by phenol red thread test and ocular surface damage assessed with fluorescein staining. Corneal homogenates were obtained for matrix metalloproteinase (MMP) and cytokine expression analysis by Luminex assay and quantitative PCR. In addition, whole eyes and eyelids were dissected and goblet cells and Meibomian glands were imaged, respectively. RESULTS: Following 5 days of EDE, WT mice had extensive ocular surface staining, while MyD88(−/−) mice had no increased staining above non-EDE conditions. Similarly, MyD88(−/−) mice did not have increased corneal MMP-2, 3, or 8 concentrations, as seen with WT mice. MyD88-deficiency also resulted in decreased corneal cytokine levels. In addition, MyD88(−/−) mice had significantly lower conjunctival goblet cell counts compared with both WT (EDE) and IL-1R(−/−) (non-EDE) mice. However, there was no difference in Meibomian gland morphology between WT, IL-1R(−/−), and MyD88(−/−) mice. CONCLUSIONS: These studies demonstrate the importance of TLR signaling in dry eye development. Mice lacking TLR signaling, MyD88(−/−), were protected from EDE-induced ocular surface damage and inflammatory mediator expression, warranting further investigation into TLR inhibition as a potential therapeutic for DED. The Association for Research in Vision and Ophthalmology 2018-06 /pmc/articles/PMC5991808/ /pubmed/30025110 http://dx.doi.org/10.1167/iovs.17-23397 Text en Copyright 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. |
spellingShingle | Immunology and Microbiology Reins, Rose Y. Lema, Carolina Courson, Justin Kunnen, Carolina M. E. Redfern, Rachel L. MyD88 Deficiency Protects Against Dry Eye–Induced Damage |
title | MyD88 Deficiency Protects Against Dry Eye–Induced Damage |
title_full | MyD88 Deficiency Protects Against Dry Eye–Induced Damage |
title_fullStr | MyD88 Deficiency Protects Against Dry Eye–Induced Damage |
title_full_unstemmed | MyD88 Deficiency Protects Against Dry Eye–Induced Damage |
title_short | MyD88 Deficiency Protects Against Dry Eye–Induced Damage |
title_sort | myd88 deficiency protects against dry eye–induced damage |
topic | Immunology and Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5991808/ https://www.ncbi.nlm.nih.gov/pubmed/30025110 http://dx.doi.org/10.1167/iovs.17-23397 |
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