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Use of Resveratrol Self-Emulsifying Systems in T/C28a2 Cell Line as Beneficial Effectors in Cellular Uptake and Protection Against Oxidative Stress-Mediated Death

Osteoarthritis (OA) is the most prevalent rheumatic disease in the world. Although its etiology is still unknown, one of the key processes in OA progression and development is oxidative stress. In this context, resveratrol, a well-known anti-oxidant from the stilbene family, could be of particular i...

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Autores principales: Le Clanche, Solenn, Cheminel, Tristan, Rannou, François, Bonnefont-Rousselot, Dominique, Borderie, Didier, Charrueau, Christine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5992418/
https://www.ncbi.nlm.nih.gov/pubmed/29910729
http://dx.doi.org/10.3389/fphar.2018.00538
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author Le Clanche, Solenn
Cheminel, Tristan
Rannou, François
Bonnefont-Rousselot, Dominique
Borderie, Didier
Charrueau, Christine
author_facet Le Clanche, Solenn
Cheminel, Tristan
Rannou, François
Bonnefont-Rousselot, Dominique
Borderie, Didier
Charrueau, Christine
author_sort Le Clanche, Solenn
collection PubMed
description Osteoarthritis (OA) is the most prevalent rheumatic disease in the world. Although its etiology is still unknown, one of the key processes in OA progression and development is oxidative stress. In this context, resveratrol, a well-known anti-oxidant from the stilbene family, could be of particular interest in future OA therapeutic strategies. However, currently, because of its low bioavailability, use of resveratrol in human health is very limited. In this study, we tested two resveratrol self-emulsifying systems previously developed in our laboratory in order to determine if they could improve cellular uptake of resveratrol in a human immortalized chondrocytic cell line (T/C28a2) and enhance protection against oxidative stress. Our results showed that resveratrol self-emulsifying systems were able first to increase cellular tolerance towards resveratrol, and thus decrease resveratrol intrinsic cellular toxicity, allowing the use of higher concentrations, second, to increase resveratrol uptake in membrane and intracellular fractions, and finally, to improve protection against oxidative stress-mediated death in human immortalized chondrocytic cell line T/C28a2. These data suggest that new formulations of resveratrol could be considered as potential beneficial effectors in future OA treatments.
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spelling pubmed-59924182018-06-15 Use of Resveratrol Self-Emulsifying Systems in T/C28a2 Cell Line as Beneficial Effectors in Cellular Uptake and Protection Against Oxidative Stress-Mediated Death Le Clanche, Solenn Cheminel, Tristan Rannou, François Bonnefont-Rousselot, Dominique Borderie, Didier Charrueau, Christine Front Pharmacol Pharmacology Osteoarthritis (OA) is the most prevalent rheumatic disease in the world. Although its etiology is still unknown, one of the key processes in OA progression and development is oxidative stress. In this context, resveratrol, a well-known anti-oxidant from the stilbene family, could be of particular interest in future OA therapeutic strategies. However, currently, because of its low bioavailability, use of resveratrol in human health is very limited. In this study, we tested two resveratrol self-emulsifying systems previously developed in our laboratory in order to determine if they could improve cellular uptake of resveratrol in a human immortalized chondrocytic cell line (T/C28a2) and enhance protection against oxidative stress. Our results showed that resveratrol self-emulsifying systems were able first to increase cellular tolerance towards resveratrol, and thus decrease resveratrol intrinsic cellular toxicity, allowing the use of higher concentrations, second, to increase resveratrol uptake in membrane and intracellular fractions, and finally, to improve protection against oxidative stress-mediated death in human immortalized chondrocytic cell line T/C28a2. These data suggest that new formulations of resveratrol could be considered as potential beneficial effectors in future OA treatments. Frontiers Media S.A. 2018-05-24 /pmc/articles/PMC5992418/ /pubmed/29910729 http://dx.doi.org/10.3389/fphar.2018.00538 Text en Copyright © 2018 Le Clanche, Cheminel, Rannou, Bonnefont-Rousselot, Borderie and Charrueau. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Le Clanche, Solenn
Cheminel, Tristan
Rannou, François
Bonnefont-Rousselot, Dominique
Borderie, Didier
Charrueau, Christine
Use of Resveratrol Self-Emulsifying Systems in T/C28a2 Cell Line as Beneficial Effectors in Cellular Uptake and Protection Against Oxidative Stress-Mediated Death
title Use of Resveratrol Self-Emulsifying Systems in T/C28a2 Cell Line as Beneficial Effectors in Cellular Uptake and Protection Against Oxidative Stress-Mediated Death
title_full Use of Resveratrol Self-Emulsifying Systems in T/C28a2 Cell Line as Beneficial Effectors in Cellular Uptake and Protection Against Oxidative Stress-Mediated Death
title_fullStr Use of Resveratrol Self-Emulsifying Systems in T/C28a2 Cell Line as Beneficial Effectors in Cellular Uptake and Protection Against Oxidative Stress-Mediated Death
title_full_unstemmed Use of Resveratrol Self-Emulsifying Systems in T/C28a2 Cell Line as Beneficial Effectors in Cellular Uptake and Protection Against Oxidative Stress-Mediated Death
title_short Use of Resveratrol Self-Emulsifying Systems in T/C28a2 Cell Line as Beneficial Effectors in Cellular Uptake and Protection Against Oxidative Stress-Mediated Death
title_sort use of resveratrol self-emulsifying systems in t/c28a2 cell line as beneficial effectors in cellular uptake and protection against oxidative stress-mediated death
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5992418/
https://www.ncbi.nlm.nih.gov/pubmed/29910729
http://dx.doi.org/10.3389/fphar.2018.00538
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