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Overexpression of Tobacco GCN2 Stimulates Multiple Physiological Changes Associated With Stress Tolerance

General control non-derepressible-2 (GCN2) is a ubiquitous protein kinase that phosphorylates the α subunit of the eukaryotic initiation factor, eIF2, preventing the initiation of a new cycle of protein synthesis, subsequently reducing the global protein biosynthesis. GCN2 can also regulate the resp...

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Autores principales: Li, Ning, Zhang, Song-jie, Zhao, Qi, Long, Yue, Guo, Hao, Jia, Hong-fang, Yang, Yong-xia, Zhang, Hong-ying, Ye, Xie-feng, Zhang, Song-tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5992440/
https://www.ncbi.nlm.nih.gov/pubmed/29910821
http://dx.doi.org/10.3389/fpls.2018.00725
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author Li, Ning
Zhang, Song-jie
Zhao, Qi
Long, Yue
Guo, Hao
Jia, Hong-fang
Yang, Yong-xia
Zhang, Hong-ying
Ye, Xie-feng
Zhang, Song-tao
author_facet Li, Ning
Zhang, Song-jie
Zhao, Qi
Long, Yue
Guo, Hao
Jia, Hong-fang
Yang, Yong-xia
Zhang, Hong-ying
Ye, Xie-feng
Zhang, Song-tao
author_sort Li, Ning
collection PubMed
description General control non-derepressible-2 (GCN2) is a ubiquitous protein kinase that phosphorylates the α subunit of the eukaryotic initiation factor, eIF2, preventing the initiation of a new cycle of protein synthesis, subsequently reducing the global protein biosynthesis. GCN2 can also regulate the response of plants to biotic and abiotic stresses. In this study, two GCN2 homologs, NtGCN2-1 and NtGCN2-2, were cloned from Nicotiana tabacum, and were predicted to have been derived from their progenitors in N. tomentosiformis and N. sylvestris, respectively. The phosphorylation of NteIF2α could be activated by promoting the expression of NtGCN2 with plant hormones, including salicylic acid (SA), azelaic acid (AZA), methyl jasmonate (MeJA), and by imposition of different stresses (Bemisia tabaci infection, drought, and cold), indicating that NtGCN2 is involved in the response of plants to multiple biotic and abiotic stresses. We also observed that the overexpression of NtGCN2-1 significantly influenced different physiological processes. It promoted seed germination and root elongation. The content of total soluble sugars and reducing sugars were decreased, whereas those of chlorophyll a and b were increased in the GCN2 overexpressing plants. In addition, the overexpressing plants had lower content of reactive oxygen species and exhibited higher antioxidant activities. These physiological alterations could be attributed to the changes in the endogenous phytohormones, decrease in the SA and abscisic acid content, and accumulation of MeJA and AZA. It indicated that the overexpression of NtGCN2 in tobacco, stimulated the plant defense responses via phosphorylation of NteIF2α and regulation of plant hormones, and changes in the antioxidant ability and plant nutrient status.
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spelling pubmed-59924402018-06-15 Overexpression of Tobacco GCN2 Stimulates Multiple Physiological Changes Associated With Stress Tolerance Li, Ning Zhang, Song-jie Zhao, Qi Long, Yue Guo, Hao Jia, Hong-fang Yang, Yong-xia Zhang, Hong-ying Ye, Xie-feng Zhang, Song-tao Front Plant Sci Plant Science General control non-derepressible-2 (GCN2) is a ubiquitous protein kinase that phosphorylates the α subunit of the eukaryotic initiation factor, eIF2, preventing the initiation of a new cycle of protein synthesis, subsequently reducing the global protein biosynthesis. GCN2 can also regulate the response of plants to biotic and abiotic stresses. In this study, two GCN2 homologs, NtGCN2-1 and NtGCN2-2, were cloned from Nicotiana tabacum, and were predicted to have been derived from their progenitors in N. tomentosiformis and N. sylvestris, respectively. The phosphorylation of NteIF2α could be activated by promoting the expression of NtGCN2 with plant hormones, including salicylic acid (SA), azelaic acid (AZA), methyl jasmonate (MeJA), and by imposition of different stresses (Bemisia tabaci infection, drought, and cold), indicating that NtGCN2 is involved in the response of plants to multiple biotic and abiotic stresses. We also observed that the overexpression of NtGCN2-1 significantly influenced different physiological processes. It promoted seed germination and root elongation. The content of total soluble sugars and reducing sugars were decreased, whereas those of chlorophyll a and b were increased in the GCN2 overexpressing plants. In addition, the overexpressing plants had lower content of reactive oxygen species and exhibited higher antioxidant activities. These physiological alterations could be attributed to the changes in the endogenous phytohormones, decrease in the SA and abscisic acid content, and accumulation of MeJA and AZA. It indicated that the overexpression of NtGCN2 in tobacco, stimulated the plant defense responses via phosphorylation of NteIF2α and regulation of plant hormones, and changes in the antioxidant ability and plant nutrient status. Frontiers Media S.A. 2018-06-01 /pmc/articles/PMC5992440/ /pubmed/29910821 http://dx.doi.org/10.3389/fpls.2018.00725 Text en Copyright © 2018 Li, Zhang, Zhao, Long, Guo, Jia, Yang, Zhang, Ye and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Li, Ning
Zhang, Song-jie
Zhao, Qi
Long, Yue
Guo, Hao
Jia, Hong-fang
Yang, Yong-xia
Zhang, Hong-ying
Ye, Xie-feng
Zhang, Song-tao
Overexpression of Tobacco GCN2 Stimulates Multiple Physiological Changes Associated With Stress Tolerance
title Overexpression of Tobacco GCN2 Stimulates Multiple Physiological Changes Associated With Stress Tolerance
title_full Overexpression of Tobacco GCN2 Stimulates Multiple Physiological Changes Associated With Stress Tolerance
title_fullStr Overexpression of Tobacco GCN2 Stimulates Multiple Physiological Changes Associated With Stress Tolerance
title_full_unstemmed Overexpression of Tobacco GCN2 Stimulates Multiple Physiological Changes Associated With Stress Tolerance
title_short Overexpression of Tobacco GCN2 Stimulates Multiple Physiological Changes Associated With Stress Tolerance
title_sort overexpression of tobacco gcn2 stimulates multiple physiological changes associated with stress tolerance
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5992440/
https://www.ncbi.nlm.nih.gov/pubmed/29910821
http://dx.doi.org/10.3389/fpls.2018.00725
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