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Osteoblast differentiation of equine induced pluripotent stem cells

Bone fractures occur in horses following traumatic and non-traumatic (bone overloading) events. They can be difficult to treat due to the need for the horse to bear weight on all legs during the healing period. Regenerative medicine to improve fracture union and recovery could significantly improve...

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Autores principales: Baird, Arabella, Lindsay, Timothy, Everett, Alice, Iyemere, Valentine, Paterson, Yasmin Z., McClellan, Alyce, Henson, Frances M. D., Guest, Deborah J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5992527/
https://www.ncbi.nlm.nih.gov/pubmed/29685993
http://dx.doi.org/10.1242/bio.033514
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author Baird, Arabella
Lindsay, Timothy
Everett, Alice
Iyemere, Valentine
Paterson, Yasmin Z.
McClellan, Alyce
Henson, Frances M. D.
Guest, Deborah J.
author_facet Baird, Arabella
Lindsay, Timothy
Everett, Alice
Iyemere, Valentine
Paterson, Yasmin Z.
McClellan, Alyce
Henson, Frances M. D.
Guest, Deborah J.
author_sort Baird, Arabella
collection PubMed
description Bone fractures occur in horses following traumatic and non-traumatic (bone overloading) events. They can be difficult to treat due to the need for the horse to bear weight on all legs during the healing period. Regenerative medicine to improve fracture union and recovery could significantly improve horse welfare. Equine induced pluripotent stem cells (iPSCs) have previously been derived. Here we show that equine iPSCs cultured for 21 days in osteogenic induction media on an OsteoAssay surface upregulate the expression of osteoblast associated genes and proteins, including COL1A1, SPARC, SPP1, IBSP, RUNX2 and BGALP. We also demonstrate that iPSC-osteoblasts are able to produce a mineralised matrix with both calcium and hydroxyapatite deposition. Alkaline phosphatase activity is also significantly increased during osteoblast differentiation. Although the genetic background of the iPSC donor animal affects the level of differentiation observed after 21 days of differentiation, less variation between lines of iPSCs derived from the same horse was observed. The successful, direct, differentiation of equine iPSCs into osteoblasts may provide a source of cells for future regenerative medicine strategies to improve fracture repair in horses undergoing surgery. iPSC-derived osteoblasts will also provide a potential tool to study equine bone development and disease.
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spelling pubmed-59925272018-06-08 Osteoblast differentiation of equine induced pluripotent stem cells Baird, Arabella Lindsay, Timothy Everett, Alice Iyemere, Valentine Paterson, Yasmin Z. McClellan, Alyce Henson, Frances M. D. Guest, Deborah J. Biol Open Research Article Bone fractures occur in horses following traumatic and non-traumatic (bone overloading) events. They can be difficult to treat due to the need for the horse to bear weight on all legs during the healing period. Regenerative medicine to improve fracture union and recovery could significantly improve horse welfare. Equine induced pluripotent stem cells (iPSCs) have previously been derived. Here we show that equine iPSCs cultured for 21 days in osteogenic induction media on an OsteoAssay surface upregulate the expression of osteoblast associated genes and proteins, including COL1A1, SPARC, SPP1, IBSP, RUNX2 and BGALP. We also demonstrate that iPSC-osteoblasts are able to produce a mineralised matrix with both calcium and hydroxyapatite deposition. Alkaline phosphatase activity is also significantly increased during osteoblast differentiation. Although the genetic background of the iPSC donor animal affects the level of differentiation observed after 21 days of differentiation, less variation between lines of iPSCs derived from the same horse was observed. The successful, direct, differentiation of equine iPSCs into osteoblasts may provide a source of cells for future regenerative medicine strategies to improve fracture repair in horses undergoing surgery. iPSC-derived osteoblasts will also provide a potential tool to study equine bone development and disease. The Company of Biologists Ltd 2018-04-23 /pmc/articles/PMC5992527/ /pubmed/29685993 http://dx.doi.org/10.1242/bio.033514 Text en © 2018. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Baird, Arabella
Lindsay, Timothy
Everett, Alice
Iyemere, Valentine
Paterson, Yasmin Z.
McClellan, Alyce
Henson, Frances M. D.
Guest, Deborah J.
Osteoblast differentiation of equine induced pluripotent stem cells
title Osteoblast differentiation of equine induced pluripotent stem cells
title_full Osteoblast differentiation of equine induced pluripotent stem cells
title_fullStr Osteoblast differentiation of equine induced pluripotent stem cells
title_full_unstemmed Osteoblast differentiation of equine induced pluripotent stem cells
title_short Osteoblast differentiation of equine induced pluripotent stem cells
title_sort osteoblast differentiation of equine induced pluripotent stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5992527/
https://www.ncbi.nlm.nih.gov/pubmed/29685993
http://dx.doi.org/10.1242/bio.033514
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