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Coordination of meristem and boundary functions by transcription factors in the SHOOT MERISTEMLESS regulatory network
The Arabidopsis homeodomain transcription factor SHOOT MERISTEMLESS (STM) is crucial for shoot apical meristem (SAM) function, yet the components and structure of the STM gene regulatory network (GRN) are largely unknown. Here, we show that transcriptional regulators are overrepresented among STM-re...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5992597/ https://www.ncbi.nlm.nih.gov/pubmed/29650590 http://dx.doi.org/10.1242/dev.157081 |
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author | Scofield, Simon Murison, Alexander Jones, Angharad Fozard, John Aida, Mitsuhiro Band, Leah R. Bennett, Malcolm Murray, James A. H. |
author_facet | Scofield, Simon Murison, Alexander Jones, Angharad Fozard, John Aida, Mitsuhiro Band, Leah R. Bennett, Malcolm Murray, James A. H. |
author_sort | Scofield, Simon |
collection | PubMed |
description | The Arabidopsis homeodomain transcription factor SHOOT MERISTEMLESS (STM) is crucial for shoot apical meristem (SAM) function, yet the components and structure of the STM gene regulatory network (GRN) are largely unknown. Here, we show that transcriptional regulators are overrepresented among STM-regulated genes and, using these as GRN components in Bayesian network analysis, we infer STM GRN associations and reveal regulatory relationships between STM and factors involved in multiple aspects of SAM function. These include hormone regulation, TCP-mediated control of cell differentiation, AIL/PLT-mediated regulation of pluripotency and phyllotaxis, and specification of meristem-organ boundary zones via CUC1. We demonstrate a direct positive transcriptional feedback loop between STM and CUC1, despite their distinct expression patterns in the meristem and organ boundary, respectively. Our further finding that STM activates expression of the CUC1-targeting microRNA miR164c combined with mathematical modelling provides a potential solution for this apparent contradiction, demonstrating that these proposed regulatory interactions coupled with STM mobility could be sufficient to provide a mechanism for CUC1 localisation at the meristem-organ boundary. Our findings highlight the central role for the STM GRN in coordinating SAM functions. |
format | Online Article Text |
id | pubmed-5992597 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59925972018-06-22 Coordination of meristem and boundary functions by transcription factors in the SHOOT MERISTEMLESS regulatory network Scofield, Simon Murison, Alexander Jones, Angharad Fozard, John Aida, Mitsuhiro Band, Leah R. Bennett, Malcolm Murray, James A. H. Development Research Article The Arabidopsis homeodomain transcription factor SHOOT MERISTEMLESS (STM) is crucial for shoot apical meristem (SAM) function, yet the components and structure of the STM gene regulatory network (GRN) are largely unknown. Here, we show that transcriptional regulators are overrepresented among STM-regulated genes and, using these as GRN components in Bayesian network analysis, we infer STM GRN associations and reveal regulatory relationships between STM and factors involved in multiple aspects of SAM function. These include hormone regulation, TCP-mediated control of cell differentiation, AIL/PLT-mediated regulation of pluripotency and phyllotaxis, and specification of meristem-organ boundary zones via CUC1. We demonstrate a direct positive transcriptional feedback loop between STM and CUC1, despite their distinct expression patterns in the meristem and organ boundary, respectively. Our further finding that STM activates expression of the CUC1-targeting microRNA miR164c combined with mathematical modelling provides a potential solution for this apparent contradiction, demonstrating that these proposed regulatory interactions coupled with STM mobility could be sufficient to provide a mechanism for CUC1 localisation at the meristem-organ boundary. Our findings highlight the central role for the STM GRN in coordinating SAM functions. The Company of Biologists Ltd 2018-05-01 2018-04-30 /pmc/articles/PMC5992597/ /pubmed/29650590 http://dx.doi.org/10.1242/dev.157081 Text en © 2018. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Scofield, Simon Murison, Alexander Jones, Angharad Fozard, John Aida, Mitsuhiro Band, Leah R. Bennett, Malcolm Murray, James A. H. Coordination of meristem and boundary functions by transcription factors in the SHOOT MERISTEMLESS regulatory network |
title | Coordination of meristem and boundary functions by transcription factors in the SHOOT MERISTEMLESS regulatory network |
title_full | Coordination of meristem and boundary functions by transcription factors in the SHOOT MERISTEMLESS regulatory network |
title_fullStr | Coordination of meristem and boundary functions by transcription factors in the SHOOT MERISTEMLESS regulatory network |
title_full_unstemmed | Coordination of meristem and boundary functions by transcription factors in the SHOOT MERISTEMLESS regulatory network |
title_short | Coordination of meristem and boundary functions by transcription factors in the SHOOT MERISTEMLESS regulatory network |
title_sort | coordination of meristem and boundary functions by transcription factors in the shoot meristemless regulatory network |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5992597/ https://www.ncbi.nlm.nih.gov/pubmed/29650590 http://dx.doi.org/10.1242/dev.157081 |
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