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Urinary exosomal expression of activator of G protein signaling 3 in polycystic kidney disease
OBJECTIVE: PKD is a genetic disease that is characterized by abnormally proliferative epithelial cells in the kidney and liver. Urinary exosomes have been previously examined as a source of unique proteins that may be used to diagnose and monitor the progression of PKD. Previous studies by our group...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5992714/ https://www.ncbi.nlm.nih.gov/pubmed/29880041 http://dx.doi.org/10.1186/s13104-018-3467-6 |
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author | Keri, Krishna C. Regner, Kevin R. Dall, Aaron T. Park, Frank |
author_facet | Keri, Krishna C. Regner, Kevin R. Dall, Aaron T. Park, Frank |
author_sort | Keri, Krishna C. |
collection | PubMed |
description | OBJECTIVE: PKD is a genetic disease that is characterized by abnormally proliferative epithelial cells in the kidney and liver. Urinary exosomes have been previously examined as a source of unique proteins that may be used to diagnose and monitor the progression of PKD. Previous studies by our group have shown that AGS3, which is a receptor-independent regulator G-proteins, was markedly upregulated in RTECs during kidney injury including PKD. In this study, our goal was to determine whether AGS3 could be measured in exosomes using animals and humans with PKD. RESULTS: In our study, urinary exosomes were isolated from PCK rats and the control Sprague–Dawley (SD) rats. AGS3 expression was significantly increased (P < 0.05) in PKD versus SD rats at 16 weeks of age. This increase was detectable in a time-dependent manner from 8 weeks of age and peaked at ~ 16–20 weeks (length of study). Similarly, in exosomes from human urine samples with PKD, AGS3 expression was significantly increased (P < 0.05) compared to healthy human controls where AGS3 was largely undetectable. In conclusion, the detection of AGS3 in urinary exosomes may be a novel biomarker for PKD, and provide new insight into the biology of tubular epithelial cell function during cystic disease progression. |
format | Online Article Text |
id | pubmed-5992714 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-59927142018-06-21 Urinary exosomal expression of activator of G protein signaling 3 in polycystic kidney disease Keri, Krishna C. Regner, Kevin R. Dall, Aaron T. Park, Frank BMC Res Notes Research Note OBJECTIVE: PKD is a genetic disease that is characterized by abnormally proliferative epithelial cells in the kidney and liver. Urinary exosomes have been previously examined as a source of unique proteins that may be used to diagnose and monitor the progression of PKD. Previous studies by our group have shown that AGS3, which is a receptor-independent regulator G-proteins, was markedly upregulated in RTECs during kidney injury including PKD. In this study, our goal was to determine whether AGS3 could be measured in exosomes using animals and humans with PKD. RESULTS: In our study, urinary exosomes were isolated from PCK rats and the control Sprague–Dawley (SD) rats. AGS3 expression was significantly increased (P < 0.05) in PKD versus SD rats at 16 weeks of age. This increase was detectable in a time-dependent manner from 8 weeks of age and peaked at ~ 16–20 weeks (length of study). Similarly, in exosomes from human urine samples with PKD, AGS3 expression was significantly increased (P < 0.05) compared to healthy human controls where AGS3 was largely undetectable. In conclusion, the detection of AGS3 in urinary exosomes may be a novel biomarker for PKD, and provide new insight into the biology of tubular epithelial cell function during cystic disease progression. BioMed Central 2018-06-07 /pmc/articles/PMC5992714/ /pubmed/29880041 http://dx.doi.org/10.1186/s13104-018-3467-6 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Note Keri, Krishna C. Regner, Kevin R. Dall, Aaron T. Park, Frank Urinary exosomal expression of activator of G protein signaling 3 in polycystic kidney disease |
title | Urinary exosomal expression of activator of G protein signaling 3 in polycystic kidney disease |
title_full | Urinary exosomal expression of activator of G protein signaling 3 in polycystic kidney disease |
title_fullStr | Urinary exosomal expression of activator of G protein signaling 3 in polycystic kidney disease |
title_full_unstemmed | Urinary exosomal expression of activator of G protein signaling 3 in polycystic kidney disease |
title_short | Urinary exosomal expression of activator of G protein signaling 3 in polycystic kidney disease |
title_sort | urinary exosomal expression of activator of g protein signaling 3 in polycystic kidney disease |
topic | Research Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5992714/ https://www.ncbi.nlm.nih.gov/pubmed/29880041 http://dx.doi.org/10.1186/s13104-018-3467-6 |
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