Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation

OBJECTIVE: This study aims to eliminate Mycoplasma spp. contamination from laboratory stocks of Chlamydia spp. by in vivo passage or by plaque assay. RESULTS: We have described two methods of eliminating Mycoplasma contamination from Chlamydia laboratory stocks. We conclude that Mycoplasma species c...

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Autores principales: Sigar, Ira M., Schripsema, Justin H., Kelly, Kathleen A., Murthy, Ashlesh K., Manam, Srikanth, Ramsey, Kyle H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5992720/
https://www.ncbi.nlm.nih.gov/pubmed/29880030
http://dx.doi.org/10.1186/s13104-018-3455-x
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author Sigar, Ira M.
Schripsema, Justin H.
Kelly, Kathleen A.
Murthy, Ashlesh K.
Manam, Srikanth
Ramsey, Kyle H.
author_facet Sigar, Ira M.
Schripsema, Justin H.
Kelly, Kathleen A.
Murthy, Ashlesh K.
Manam, Srikanth
Ramsey, Kyle H.
author_sort Sigar, Ira M.
collection PubMed
description OBJECTIVE: This study aims to eliminate Mycoplasma spp. contamination from laboratory stocks of Chlamydia spp. by in vivo passage or by plaque assay. RESULTS: We have described two methods of eliminating Mycoplasma contamination from Chlamydia laboratory stocks. We conclude that Mycoplasma species commonly contaminating chlamydial stocks do not survive passage in mice. Chlamydia may also be derived Mycoplasma-free by plaque assay. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-018-3455-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-59927202018-06-21 Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation Sigar, Ira M. Schripsema, Justin H. Kelly, Kathleen A. Murthy, Ashlesh K. Manam, Srikanth Ramsey, Kyle H. BMC Res Notes Research Note OBJECTIVE: This study aims to eliminate Mycoplasma spp. contamination from laboratory stocks of Chlamydia spp. by in vivo passage or by plaque assay. RESULTS: We have described two methods of eliminating Mycoplasma contamination from Chlamydia laboratory stocks. We conclude that Mycoplasma species commonly contaminating chlamydial stocks do not survive passage in mice. Chlamydia may also be derived Mycoplasma-free by plaque assay. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-018-3455-x) contains supplementary material, which is available to authorized users. BioMed Central 2018-06-07 /pmc/articles/PMC5992720/ /pubmed/29880030 http://dx.doi.org/10.1186/s13104-018-3455-x Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Sigar, Ira M.
Schripsema, Justin H.
Kelly, Kathleen A.
Murthy, Ashlesh K.
Manam, Srikanth
Ramsey, Kyle H.
Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation
title Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation
title_full Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation
title_fullStr Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation
title_full_unstemmed Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation
title_short Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation
title_sort elimination of mycoplasma contamination in chlamydia stocks as a result of in vivo passage or plaque isolation
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5992720/
https://www.ncbi.nlm.nih.gov/pubmed/29880030
http://dx.doi.org/10.1186/s13104-018-3455-x
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