Cargando…

Decreased expression of SRY-box containing gene 30 is related to malignant phenotypes of human bladder cancer and correlates with poor prognosis

BACKGROUND: In human pulmonary malignancies, the SRY-box containing gene 30 (SOX30) is a known cancer-suppressing gene. Nevertheless, its molecular role and clinical effects remains unknown in bladder cancer. METHODS: SOX30 mRNA expression was quantified in bladder cancer tissue, paired adjacent nor...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Yang, Wang, Han, Zhong, Jianhua, Wu, Chenglong, Yang, Gang, Zhong, Yuantang, Zhang, Jinghua, Tang, Aifa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5992861/
https://www.ncbi.nlm.nih.gov/pubmed/29880037
http://dx.doi.org/10.1186/s12885-018-4560-x
_version_ 1783330122988781568
author Liu, Yang
Wang, Han
Zhong, Jianhua
Wu, Chenglong
Yang, Gang
Zhong, Yuantang
Zhang, Jinghua
Tang, Aifa
author_facet Liu, Yang
Wang, Han
Zhong, Jianhua
Wu, Chenglong
Yang, Gang
Zhong, Yuantang
Zhang, Jinghua
Tang, Aifa
author_sort Liu, Yang
collection PubMed
description BACKGROUND: In human pulmonary malignancies, the SRY-box containing gene 30 (SOX30) is a known cancer-suppressing gene. Nevertheless, its molecular role and clinical effects remains unknown in bladder cancer. METHODS: SOX30 mRNA expression was quantified in bladder cancer tissue, paired adjacent normal tissue, and cell lines with qRT-PCR. SOX30 protein expression in BC tissue and cell lines was evaluated via western blotting and immunohistochemistry. In addition, the clinical and prognostic significance of SOX30 in BC were assessed using Kaplan-Meier analysis. Furthermore, we measured cell migration and invasion, cell proliferation and cell apoptosis by means of a Transwell assay, cell counting kit-8 along with flow cytometry, respectively. RESULTS: Expression levels of SOX30 were markedly lower in BC cells and tumor tissues than in adjacent noncancerous tissues. Moreover, clinicopathological analyses showed that low SOX30 expression was positively related to an advanced tumor, node, and metastasis (TNM) stage. Furthermore, low SOX30 expression conferred reduced survival rates (P < 0.05). Functional analyses revealed that SOX30 overexpression attenuated cell proliferation, invasion, and migration, while promoting apoptosis in BC cells. CONCLUSIONS: SOX30 displays tumor suppressive behavior, warranting future investigations into its therapeutic potential in the treatment of BC.
format Online
Article
Text
id pubmed-5992861
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-59928612018-07-05 Decreased expression of SRY-box containing gene 30 is related to malignant phenotypes of human bladder cancer and correlates with poor prognosis Liu, Yang Wang, Han Zhong, Jianhua Wu, Chenglong Yang, Gang Zhong, Yuantang Zhang, Jinghua Tang, Aifa BMC Cancer Research Article BACKGROUND: In human pulmonary malignancies, the SRY-box containing gene 30 (SOX30) is a known cancer-suppressing gene. Nevertheless, its molecular role and clinical effects remains unknown in bladder cancer. METHODS: SOX30 mRNA expression was quantified in bladder cancer tissue, paired adjacent normal tissue, and cell lines with qRT-PCR. SOX30 protein expression in BC tissue and cell lines was evaluated via western blotting and immunohistochemistry. In addition, the clinical and prognostic significance of SOX30 in BC were assessed using Kaplan-Meier analysis. Furthermore, we measured cell migration and invasion, cell proliferation and cell apoptosis by means of a Transwell assay, cell counting kit-8 along with flow cytometry, respectively. RESULTS: Expression levels of SOX30 were markedly lower in BC cells and tumor tissues than in adjacent noncancerous tissues. Moreover, clinicopathological analyses showed that low SOX30 expression was positively related to an advanced tumor, node, and metastasis (TNM) stage. Furthermore, low SOX30 expression conferred reduced survival rates (P < 0.05). Functional analyses revealed that SOX30 overexpression attenuated cell proliferation, invasion, and migration, while promoting apoptosis in BC cells. CONCLUSIONS: SOX30 displays tumor suppressive behavior, warranting future investigations into its therapeutic potential in the treatment of BC. BioMed Central 2018-06-07 /pmc/articles/PMC5992861/ /pubmed/29880037 http://dx.doi.org/10.1186/s12885-018-4560-x Text en © The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Liu, Yang
Wang, Han
Zhong, Jianhua
Wu, Chenglong
Yang, Gang
Zhong, Yuantang
Zhang, Jinghua
Tang, Aifa
Decreased expression of SRY-box containing gene 30 is related to malignant phenotypes of human bladder cancer and correlates with poor prognosis
title Decreased expression of SRY-box containing gene 30 is related to malignant phenotypes of human bladder cancer and correlates with poor prognosis
title_full Decreased expression of SRY-box containing gene 30 is related to malignant phenotypes of human bladder cancer and correlates with poor prognosis
title_fullStr Decreased expression of SRY-box containing gene 30 is related to malignant phenotypes of human bladder cancer and correlates with poor prognosis
title_full_unstemmed Decreased expression of SRY-box containing gene 30 is related to malignant phenotypes of human bladder cancer and correlates with poor prognosis
title_short Decreased expression of SRY-box containing gene 30 is related to malignant phenotypes of human bladder cancer and correlates with poor prognosis
title_sort decreased expression of sry-box containing gene 30 is related to malignant phenotypes of human bladder cancer and correlates with poor prognosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5992861/
https://www.ncbi.nlm.nih.gov/pubmed/29880037
http://dx.doi.org/10.1186/s12885-018-4560-x
work_keys_str_mv AT liuyang decreasedexpressionofsryboxcontaininggene30isrelatedtomalignantphenotypesofhumanbladdercancerandcorrelateswithpoorprognosis
AT wanghan decreasedexpressionofsryboxcontaininggene30isrelatedtomalignantphenotypesofhumanbladdercancerandcorrelateswithpoorprognosis
AT zhongjianhua decreasedexpressionofsryboxcontaininggene30isrelatedtomalignantphenotypesofhumanbladdercancerandcorrelateswithpoorprognosis
AT wuchenglong decreasedexpressionofsryboxcontaininggene30isrelatedtomalignantphenotypesofhumanbladdercancerandcorrelateswithpoorprognosis
AT yanggang decreasedexpressionofsryboxcontaininggene30isrelatedtomalignantphenotypesofhumanbladdercancerandcorrelateswithpoorprognosis
AT zhongyuantang decreasedexpressionofsryboxcontaininggene30isrelatedtomalignantphenotypesofhumanbladdercancerandcorrelateswithpoorprognosis
AT zhangjinghua decreasedexpressionofsryboxcontaininggene30isrelatedtomalignantphenotypesofhumanbladdercancerandcorrelateswithpoorprognosis
AT tangaifa decreasedexpressionofsryboxcontaininggene30isrelatedtomalignantphenotypesofhumanbladdercancerandcorrelateswithpoorprognosis