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Synaptic Potential and Plasticity of an SK2 Channel Gate Regulate Spike Burst Activity in Cerebellar Purkinje Cells

Neurons store information and participate in memory engrams as a result of experience-dependent changes in synaptic weights and in membrane excitability. Here, we examine excitatory postsynaptic potential (EPSP) amplitude and neuronal excitability in relation to these two mechanisms of plasticity. W...

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Detalles Bibliográficos
Autores principales: Ohtsuki, Gen, Hansel, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5993052/
https://www.ncbi.nlm.nih.gov/pubmed/29888747
http://dx.doi.org/10.1016/j.isci.2018.02.001
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author Ohtsuki, Gen
Hansel, Christian
author_facet Ohtsuki, Gen
Hansel, Christian
author_sort Ohtsuki, Gen
collection PubMed
description Neurons store information and participate in memory engrams as a result of experience-dependent changes in synaptic weights and in membrane excitability. Here, we examine excitatory postsynaptic potential (EPSP) amplitude and neuronal excitability in relation to these two mechanisms of plasticity. We analyze somato-dendritic double-patch recordings from cerebellar Purkinje cells while inducing intrinsic, SK2 channel-dependent plasticity or blocking SK channels with bath application of apamin. Both manipulations increase the build-up of EPSP amplitudes during an EPSP train and enhance the number of EPSP-evoked spikes, yielding insights into the mechanistic contribution of EPSP amplitude to single spikes and spike bursts. EPSP amplitude has an impact on whether spikes are fired or not, but direct measures of excitability (spike threshold/AHP) are better predictors of whether individual spikes or spike bursts are fired. Our findings show that Purkinje cell spiking is synaptically driven but that burst firing is gated by SK2 channel modulation and plasticity.
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spelling pubmed-59930522018-06-08 Synaptic Potential and Plasticity of an SK2 Channel Gate Regulate Spike Burst Activity in Cerebellar Purkinje Cells Ohtsuki, Gen Hansel, Christian iScience Article Neurons store information and participate in memory engrams as a result of experience-dependent changes in synaptic weights and in membrane excitability. Here, we examine excitatory postsynaptic potential (EPSP) amplitude and neuronal excitability in relation to these two mechanisms of plasticity. We analyze somato-dendritic double-patch recordings from cerebellar Purkinje cells while inducing intrinsic, SK2 channel-dependent plasticity or blocking SK channels with bath application of apamin. Both manipulations increase the build-up of EPSP amplitudes during an EPSP train and enhance the number of EPSP-evoked spikes, yielding insights into the mechanistic contribution of EPSP amplitude to single spikes and spike bursts. EPSP amplitude has an impact on whether spikes are fired or not, but direct measures of excitability (spike threshold/AHP) are better predictors of whether individual spikes or spike bursts are fired. Our findings show that Purkinje cell spiking is synaptically driven but that burst firing is gated by SK2 channel modulation and plasticity. Elsevier 2018-03-08 /pmc/articles/PMC5993052/ /pubmed/29888747 http://dx.doi.org/10.1016/j.isci.2018.02.001 Text en © 2018 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Ohtsuki, Gen
Hansel, Christian
Synaptic Potential and Plasticity of an SK2 Channel Gate Regulate Spike Burst Activity in Cerebellar Purkinje Cells
title Synaptic Potential and Plasticity of an SK2 Channel Gate Regulate Spike Burst Activity in Cerebellar Purkinje Cells
title_full Synaptic Potential and Plasticity of an SK2 Channel Gate Regulate Spike Burst Activity in Cerebellar Purkinje Cells
title_fullStr Synaptic Potential and Plasticity of an SK2 Channel Gate Regulate Spike Burst Activity in Cerebellar Purkinje Cells
title_full_unstemmed Synaptic Potential and Plasticity of an SK2 Channel Gate Regulate Spike Burst Activity in Cerebellar Purkinje Cells
title_short Synaptic Potential and Plasticity of an SK2 Channel Gate Regulate Spike Burst Activity in Cerebellar Purkinje Cells
title_sort synaptic potential and plasticity of an sk2 channel gate regulate spike burst activity in cerebellar purkinje cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5993052/
https://www.ncbi.nlm.nih.gov/pubmed/29888747
http://dx.doi.org/10.1016/j.isci.2018.02.001
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