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Expression, regulation and function of miR-126 in the mouse choroid vasculature

MicroRNA miR-126 has been shown to be required for proper angiogenesis in several models. However, its expression, regulation and function in the mouse choroid remain unclear. Our previous data has shown that miR-126 expression is enriched in the endothelial cells (ECs) of the mouse choroid. Here we...

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Autores principales: Zhao, Fangkun, Anderson, Chastain, Karnes, Sara, Zhou, Qinbo, Ma, Jing, Jin, Zheng-Gen, Bhattacharjee, Partha S., Wang, Shusheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5993209/
https://www.ncbi.nlm.nih.gov/pubmed/29501382
http://dx.doi.org/10.1016/j.exer.2018.02.026
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author Zhao, Fangkun
Anderson, Chastain
Karnes, Sara
Zhou, Qinbo
Ma, Jing
Jin, Zheng-Gen
Bhattacharjee, Partha S.
Wang, Shusheng
author_facet Zhao, Fangkun
Anderson, Chastain
Karnes, Sara
Zhou, Qinbo
Ma, Jing
Jin, Zheng-Gen
Bhattacharjee, Partha S.
Wang, Shusheng
author_sort Zhao, Fangkun
collection PubMed
description MicroRNA miR-126 has been shown to be required for proper angiogenesis in several models. However, its expression, regulation and function in the mouse choroid remain unclear. Our previous data has shown that miR-126 expression is enriched in the endothelial cells (ECs) of the mouse choroid. Here we report that a 5.5 kb Egfl7/miR-126 promoter drives the expression of miR-126 in the choroid ECs during choroidal vascular development. The expression of miR-126 in the ECs is regulated by flow stress likely through Krüppel-like transcriptional factors. miR-126(−/−) mice show mildly delayed choroidal vascular development, but adult knockout mice develop periphery choroidal vascular lesions. This study suggests that miR-126 is largely dispensable for mouse choroidal development but required for maintaining choroidal vasculature integrity.
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spelling pubmed-59932092018-06-08 Expression, regulation and function of miR-126 in the mouse choroid vasculature Zhao, Fangkun Anderson, Chastain Karnes, Sara Zhou, Qinbo Ma, Jing Jin, Zheng-Gen Bhattacharjee, Partha S. Wang, Shusheng Exp Eye Res Article MicroRNA miR-126 has been shown to be required for proper angiogenesis in several models. However, its expression, regulation and function in the mouse choroid remain unclear. Our previous data has shown that miR-126 expression is enriched in the endothelial cells (ECs) of the mouse choroid. Here we report that a 5.5 kb Egfl7/miR-126 promoter drives the expression of miR-126 in the choroid ECs during choroidal vascular development. The expression of miR-126 in the ECs is regulated by flow stress likely through Krüppel-like transcriptional factors. miR-126(−/−) mice show mildly delayed choroidal vascular development, but adult knockout mice develop periphery choroidal vascular lesions. This study suggests that miR-126 is largely dispensable for mouse choroidal development but required for maintaining choroidal vasculature integrity. 2018-03-01 2018-05 /pmc/articles/PMC5993209/ /pubmed/29501382 http://dx.doi.org/10.1016/j.exer.2018.02.026 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Zhao, Fangkun
Anderson, Chastain
Karnes, Sara
Zhou, Qinbo
Ma, Jing
Jin, Zheng-Gen
Bhattacharjee, Partha S.
Wang, Shusheng
Expression, regulation and function of miR-126 in the mouse choroid vasculature
title Expression, regulation and function of miR-126 in the mouse choroid vasculature
title_full Expression, regulation and function of miR-126 in the mouse choroid vasculature
title_fullStr Expression, regulation and function of miR-126 in the mouse choroid vasculature
title_full_unstemmed Expression, regulation and function of miR-126 in the mouse choroid vasculature
title_short Expression, regulation and function of miR-126 in the mouse choroid vasculature
title_sort expression, regulation and function of mir-126 in the mouse choroid vasculature
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5993209/
https://www.ncbi.nlm.nih.gov/pubmed/29501382
http://dx.doi.org/10.1016/j.exer.2018.02.026
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