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Wireless control of cellular function by activation of a novel protein responsive to electromagnetic fields
The Kryptopterus bicirrhis (glass catfish) is known to respond to electromagnetic fields (EMF). Here we tested its avoidance behavior in response to static and alternating magnetic fields stimulation. Using expression cloning we identified an electromagnetic perceptive gene (EPG) from the K. bicirrh...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5993716/ https://www.ncbi.nlm.nih.gov/pubmed/29884813 http://dx.doi.org/10.1038/s41598-018-27087-9 |
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author | Krishnan, Vijai Park, Sarah A. Shin, Samuel S. Alon, Lina Tressler, Caitlin M. Stokes, William Banerjee, Jineta Sorrell, Mary E. Tian, Yuemin Fridman, Gene Y. Celnik, Pablo Pevsner, Jonathan Guggino, William B. Gilad, Assaf A. Pelled, Galit |
author_facet | Krishnan, Vijai Park, Sarah A. Shin, Samuel S. Alon, Lina Tressler, Caitlin M. Stokes, William Banerjee, Jineta Sorrell, Mary E. Tian, Yuemin Fridman, Gene Y. Celnik, Pablo Pevsner, Jonathan Guggino, William B. Gilad, Assaf A. Pelled, Galit |
author_sort | Krishnan, Vijai |
collection | PubMed |
description | The Kryptopterus bicirrhis (glass catfish) is known to respond to electromagnetic fields (EMF). Here we tested its avoidance behavior in response to static and alternating magnetic fields stimulation. Using expression cloning we identified an electromagnetic perceptive gene (EPG) from the K. bicirrhis encoding a protein that responds to EMF. This EPG gene was cloned and expressed in mammalian cells, neuronal cultures and in rat’s brain. Immunohistochemistry showed that the expression of EPG is confined to the mammalian cell membrane. Calcium imaging in mammalian cells and cultured neurons expressing EPG demonstrated that remote activation by EMF significantly increases intracellular calcium concentrations, indicative of cellular excitability. Moreover, wireless magnetic activation of EPG in rat motor cortex induced motor evoked responses of the contralateral forelimb in vivo. Here we report on the development of a new technology for remote, non-invasive modulation of cell function. |
format | Online Article Text |
id | pubmed-5993716 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59937162018-07-05 Wireless control of cellular function by activation of a novel protein responsive to electromagnetic fields Krishnan, Vijai Park, Sarah A. Shin, Samuel S. Alon, Lina Tressler, Caitlin M. Stokes, William Banerjee, Jineta Sorrell, Mary E. Tian, Yuemin Fridman, Gene Y. Celnik, Pablo Pevsner, Jonathan Guggino, William B. Gilad, Assaf A. Pelled, Galit Sci Rep Article The Kryptopterus bicirrhis (glass catfish) is known to respond to electromagnetic fields (EMF). Here we tested its avoidance behavior in response to static and alternating magnetic fields stimulation. Using expression cloning we identified an electromagnetic perceptive gene (EPG) from the K. bicirrhis encoding a protein that responds to EMF. This EPG gene was cloned and expressed in mammalian cells, neuronal cultures and in rat’s brain. Immunohistochemistry showed that the expression of EPG is confined to the mammalian cell membrane. Calcium imaging in mammalian cells and cultured neurons expressing EPG demonstrated that remote activation by EMF significantly increases intracellular calcium concentrations, indicative of cellular excitability. Moreover, wireless magnetic activation of EPG in rat motor cortex induced motor evoked responses of the contralateral forelimb in vivo. Here we report on the development of a new technology for remote, non-invasive modulation of cell function. Nature Publishing Group UK 2018-06-08 /pmc/articles/PMC5993716/ /pubmed/29884813 http://dx.doi.org/10.1038/s41598-018-27087-9 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Krishnan, Vijai Park, Sarah A. Shin, Samuel S. Alon, Lina Tressler, Caitlin M. Stokes, William Banerjee, Jineta Sorrell, Mary E. Tian, Yuemin Fridman, Gene Y. Celnik, Pablo Pevsner, Jonathan Guggino, William B. Gilad, Assaf A. Pelled, Galit Wireless control of cellular function by activation of a novel protein responsive to electromagnetic fields |
title | Wireless control of cellular function by activation of a novel protein responsive to electromagnetic fields |
title_full | Wireless control of cellular function by activation of a novel protein responsive to electromagnetic fields |
title_fullStr | Wireless control of cellular function by activation of a novel protein responsive to electromagnetic fields |
title_full_unstemmed | Wireless control of cellular function by activation of a novel protein responsive to electromagnetic fields |
title_short | Wireless control of cellular function by activation of a novel protein responsive to electromagnetic fields |
title_sort | wireless control of cellular function by activation of a novel protein responsive to electromagnetic fields |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5993716/ https://www.ncbi.nlm.nih.gov/pubmed/29884813 http://dx.doi.org/10.1038/s41598-018-27087-9 |
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