CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing

CRISPR advances genome engineering by directing endonuclease sequence specificity with a guide RNA molecule (gRNA). For precisely targeting a gene for modification, each genetic construct requires a unique gRNA. By generating a gRNA against the flippase recognition target (FRT) site, a common geneti...

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Detalles Bibliográficos
Autores principales: Swings, Toon, Marciano, David C., Atri, Benu, Bosserman, Rachel E., Wang, Chen, Leysen, Marlies, Bonte, Camille, Schalck, Thomas, Furey, Ian, Van den Bergh, Bram, Verstraeten, Natalie, Christie, Peter J., Herman, Christophe, Lichtarge, Olivier, Michiels, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5993718/
https://www.ncbi.nlm.nih.gov/pubmed/29884781
http://dx.doi.org/10.1038/s41467-018-04651-5
Descripción
Sumario:CRISPR advances genome engineering by directing endonuclease sequence specificity with a guide RNA molecule (gRNA). For precisely targeting a gene for modification, each genetic construct requires a unique gRNA. By generating a gRNA against the flippase recognition target (FRT) site, a common genetic element shared by multiple genetic collections, CRISPR-FRT circumvents this design constraint to provide a broad platform for fast, scarless, off-the-shelf genome engineering.

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