CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing

CRISPR advances genome engineering by directing endonuclease sequence specificity with a guide RNA molecule (gRNA). For precisely targeting a gene for modification, each genetic construct requires a unique gRNA. By generating a gRNA against the flippase recognition target (FRT) site, a common geneti...

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Autores principales: Swings, Toon, Marciano, David C., Atri, Benu, Bosserman, Rachel E., Wang, Chen, Leysen, Marlies, Bonte, Camille, Schalck, Thomas, Furey, Ian, Van den Bergh, Bram, Verstraeten, Natalie, Christie, Peter J., Herman, Christophe, Lichtarge, Olivier, Michiels, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5993718/
https://www.ncbi.nlm.nih.gov/pubmed/29884781
http://dx.doi.org/10.1038/s41467-018-04651-5
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author Swings, Toon
Marciano, David C.
Atri, Benu
Bosserman, Rachel E.
Wang, Chen
Leysen, Marlies
Bonte, Camille
Schalck, Thomas
Furey, Ian
Van den Bergh, Bram
Verstraeten, Natalie
Christie, Peter J.
Herman, Christophe
Lichtarge, Olivier
Michiels, Jan
author_facet Swings, Toon
Marciano, David C.
Atri, Benu
Bosserman, Rachel E.
Wang, Chen
Leysen, Marlies
Bonte, Camille
Schalck, Thomas
Furey, Ian
Van den Bergh, Bram
Verstraeten, Natalie
Christie, Peter J.
Herman, Christophe
Lichtarge, Olivier
Michiels, Jan
author_sort Swings, Toon
collection PubMed
description CRISPR advances genome engineering by directing endonuclease sequence specificity with a guide RNA molecule (gRNA). For precisely targeting a gene for modification, each genetic construct requires a unique gRNA. By generating a gRNA against the flippase recognition target (FRT) site, a common genetic element shared by multiple genetic collections, CRISPR-FRT circumvents this design constraint to provide a broad platform for fast, scarless, off-the-shelf genome engineering.
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spelling pubmed-59937182018-06-11 CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing Swings, Toon Marciano, David C. Atri, Benu Bosserman, Rachel E. Wang, Chen Leysen, Marlies Bonte, Camille Schalck, Thomas Furey, Ian Van den Bergh, Bram Verstraeten, Natalie Christie, Peter J. Herman, Christophe Lichtarge, Olivier Michiels, Jan Nat Commun Article CRISPR advances genome engineering by directing endonuclease sequence specificity with a guide RNA molecule (gRNA). For precisely targeting a gene for modification, each genetic construct requires a unique gRNA. By generating a gRNA against the flippase recognition target (FRT) site, a common genetic element shared by multiple genetic collections, CRISPR-FRT circumvents this design constraint to provide a broad platform for fast, scarless, off-the-shelf genome engineering. Nature Publishing Group UK 2018-06-08 /pmc/articles/PMC5993718/ /pubmed/29884781 http://dx.doi.org/10.1038/s41467-018-04651-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Swings, Toon
Marciano, David C.
Atri, Benu
Bosserman, Rachel E.
Wang, Chen
Leysen, Marlies
Bonte, Camille
Schalck, Thomas
Furey, Ian
Van den Bergh, Bram
Verstraeten, Natalie
Christie, Peter J.
Herman, Christophe
Lichtarge, Olivier
Michiels, Jan
CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing
title CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing
title_full CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing
title_fullStr CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing
title_full_unstemmed CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing
title_short CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing
title_sort crispr-frt targets shared sites in a knock-out collection for off-the-shelf genome editing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5993718/
https://www.ncbi.nlm.nih.gov/pubmed/29884781
http://dx.doi.org/10.1038/s41467-018-04651-5
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