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The biogeochemical fate of nickel during microbial ISA degradation; implications for nuclear waste disposal
Intermediate level radioactive waste (ILW) generally contains a heterogeneous range of organic and inorganic materials, of which some are encapsulated in cement. Of particular concern are cellulosic waste items, which will chemically degrade under the conditions predicted during waste disposal, form...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5993814/ https://www.ncbi.nlm.nih.gov/pubmed/29884890 http://dx.doi.org/10.1038/s41598-018-26963-8 |
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author | Kuippers, Gina Boothman, Christopher Bagshaw, Heath Ward, Michael Beard, Rebecca Bryan, Nicholas Lloyd, Jonathan R. |
author_facet | Kuippers, Gina Boothman, Christopher Bagshaw, Heath Ward, Michael Beard, Rebecca Bryan, Nicholas Lloyd, Jonathan R. |
author_sort | Kuippers, Gina |
collection | PubMed |
description | Intermediate level radioactive waste (ILW) generally contains a heterogeneous range of organic and inorganic materials, of which some are encapsulated in cement. Of particular concern are cellulosic waste items, which will chemically degrade under the conditions predicted during waste disposal, forming significant quantities of isosaccharinic acid (ISA), a strongly chelating ligand. ISA therefore has the potential to increase the mobility of a wide range of radionuclides via complex formation, including Ni-63 and Ni-59. Although ISA is known to be metabolized by anaerobic microorganisms, the biodegradation of metal-ISA complexes remains unexplored. This study investigates the fate of a Ni-ISA complex in Fe(III)-reducing enrichment cultures at neutral pH, representative of a microbial community in the subsurface. After initial sorption of Ni onto Fe(III)oxyhydroxides, microbial ISA biodegradation resulted in >90% removal of the remaining Ni from solution when present at 0.1 mM, whereas higher concentrations of Ni proved toxic. The microbial consortium associated with ISA degradation was dominated by close relatives to Clostridia and Geobacter species. Nickel was preferentially immobilized with trace amounts of biogenic amorphous iron sulfides. This study highlights the potential for microbial activity to help remove chelating agents and radionuclides from the groundwater in the subsurface geosphere surrounding a geodisposal facility. |
format | Online Article Text |
id | pubmed-5993814 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59938142018-06-21 The biogeochemical fate of nickel during microbial ISA degradation; implications for nuclear waste disposal Kuippers, Gina Boothman, Christopher Bagshaw, Heath Ward, Michael Beard, Rebecca Bryan, Nicholas Lloyd, Jonathan R. Sci Rep Article Intermediate level radioactive waste (ILW) generally contains a heterogeneous range of organic and inorganic materials, of which some are encapsulated in cement. Of particular concern are cellulosic waste items, which will chemically degrade under the conditions predicted during waste disposal, forming significant quantities of isosaccharinic acid (ISA), a strongly chelating ligand. ISA therefore has the potential to increase the mobility of a wide range of radionuclides via complex formation, including Ni-63 and Ni-59. Although ISA is known to be metabolized by anaerobic microorganisms, the biodegradation of metal-ISA complexes remains unexplored. This study investigates the fate of a Ni-ISA complex in Fe(III)-reducing enrichment cultures at neutral pH, representative of a microbial community in the subsurface. After initial sorption of Ni onto Fe(III)oxyhydroxides, microbial ISA biodegradation resulted in >90% removal of the remaining Ni from solution when present at 0.1 mM, whereas higher concentrations of Ni proved toxic. The microbial consortium associated with ISA degradation was dominated by close relatives to Clostridia and Geobacter species. Nickel was preferentially immobilized with trace amounts of biogenic amorphous iron sulfides. This study highlights the potential for microbial activity to help remove chelating agents and radionuclides from the groundwater in the subsurface geosphere surrounding a geodisposal facility. Nature Publishing Group UK 2018-06-08 /pmc/articles/PMC5993814/ /pubmed/29884890 http://dx.doi.org/10.1038/s41598-018-26963-8 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kuippers, Gina Boothman, Christopher Bagshaw, Heath Ward, Michael Beard, Rebecca Bryan, Nicholas Lloyd, Jonathan R. The biogeochemical fate of nickel during microbial ISA degradation; implications for nuclear waste disposal |
title | The biogeochemical fate of nickel during microbial ISA degradation; implications for nuclear waste disposal |
title_full | The biogeochemical fate of nickel during microbial ISA degradation; implications for nuclear waste disposal |
title_fullStr | The biogeochemical fate of nickel during microbial ISA degradation; implications for nuclear waste disposal |
title_full_unstemmed | The biogeochemical fate of nickel during microbial ISA degradation; implications for nuclear waste disposal |
title_short | The biogeochemical fate of nickel during microbial ISA degradation; implications for nuclear waste disposal |
title_sort | biogeochemical fate of nickel during microbial isa degradation; implications for nuclear waste disposal |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5993814/ https://www.ncbi.nlm.nih.gov/pubmed/29884890 http://dx.doi.org/10.1038/s41598-018-26963-8 |
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