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Intravitreal Bevacizumab Injection Attenuates Diabetic Retinopathy in Adult Rats with Experimentally Induced Diabetes in the Early Stage
Diabetic retinopathy is the leading cause of blindness, yet its treatment is very limited. Anti-VEGF drug has been widely applied in ocular disease, but its effects on diabetic retinopathy and the underlying mechanism have remained to be fully explored. To elucidate the role of anti-VEGF treatment,...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5994287/ https://www.ncbi.nlm.nih.gov/pubmed/29977927 http://dx.doi.org/10.1155/2018/9216791 |
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author | Lv, Jiao Chen, Miao-Miao Mu, Zhi-Hao Wang, Fang Qian, Zhong-Yi Zhou, Lei Guo, Qiu-Ting Zhao, Zhi-Min Pan, Yu-Ping Liao, Xin-Yu Yang, Zhi-Hong Cai, Ning Li, Shu-De Zou, Ying-Ying |
author_facet | Lv, Jiao Chen, Miao-Miao Mu, Zhi-Hao Wang, Fang Qian, Zhong-Yi Zhou, Lei Guo, Qiu-Ting Zhao, Zhi-Min Pan, Yu-Ping Liao, Xin-Yu Yang, Zhi-Hong Cai, Ning Li, Shu-De Zou, Ying-Ying |
author_sort | Lv, Jiao |
collection | PubMed |
description | Diabetic retinopathy is the leading cause of blindness, yet its treatment is very limited. Anti-VEGF drug has been widely applied in ocular disease, but its effects on diabetic retinopathy and the underlying mechanism have remained to be fully explored. To elucidate the role of anti-VEGF treatment, we sought to determine the effects of bevacizumab on diabetic neurovascular changes extending from the 3rd to 9th week with induced diabetes in adult rats. The retinal neurovascular changes included increased expression of VEGF, nNOS, iNOS, eNOS, and NO in the course of diabetes progression. In diabetic rats given bevacizumab injection, the ganglion cell loss and alterations of retinal thickness were ameliorated. In this connection, the immunofluorescence labeling of the above biomarkers was noticeably decreased. Along with this, Western blotting confirmed that bevacizumab treatment was associated with a decrease of VEGF, Flk-1, and cAMP response element binding and protein kinase C protein expression. The present results suggest that bevacizumab treatment in the early stage of the retinopathy may ameliorate the lesions of retinopathy, in which VEGF/Flk-1 signaling has been shown here to play an important role. |
format | Online Article Text |
id | pubmed-5994287 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-59942872018-07-05 Intravitreal Bevacizumab Injection Attenuates Diabetic Retinopathy in Adult Rats with Experimentally Induced Diabetes in the Early Stage Lv, Jiao Chen, Miao-Miao Mu, Zhi-Hao Wang, Fang Qian, Zhong-Yi Zhou, Lei Guo, Qiu-Ting Zhao, Zhi-Min Pan, Yu-Ping Liao, Xin-Yu Yang, Zhi-Hong Cai, Ning Li, Shu-De Zou, Ying-Ying J Diabetes Res Research Article Diabetic retinopathy is the leading cause of blindness, yet its treatment is very limited. Anti-VEGF drug has been widely applied in ocular disease, but its effects on diabetic retinopathy and the underlying mechanism have remained to be fully explored. To elucidate the role of anti-VEGF treatment, we sought to determine the effects of bevacizumab on diabetic neurovascular changes extending from the 3rd to 9th week with induced diabetes in adult rats. The retinal neurovascular changes included increased expression of VEGF, nNOS, iNOS, eNOS, and NO in the course of diabetes progression. In diabetic rats given bevacizumab injection, the ganglion cell loss and alterations of retinal thickness were ameliorated. In this connection, the immunofluorescence labeling of the above biomarkers was noticeably decreased. Along with this, Western blotting confirmed that bevacizumab treatment was associated with a decrease of VEGF, Flk-1, and cAMP response element binding and protein kinase C protein expression. The present results suggest that bevacizumab treatment in the early stage of the retinopathy may ameliorate the lesions of retinopathy, in which VEGF/Flk-1 signaling has been shown here to play an important role. Hindawi 2018-05-09 /pmc/articles/PMC5994287/ /pubmed/29977927 http://dx.doi.org/10.1155/2018/9216791 Text en Copyright © 2018 Jiao Lv et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Lv, Jiao Chen, Miao-Miao Mu, Zhi-Hao Wang, Fang Qian, Zhong-Yi Zhou, Lei Guo, Qiu-Ting Zhao, Zhi-Min Pan, Yu-Ping Liao, Xin-Yu Yang, Zhi-Hong Cai, Ning Li, Shu-De Zou, Ying-Ying Intravitreal Bevacizumab Injection Attenuates Diabetic Retinopathy in Adult Rats with Experimentally Induced Diabetes in the Early Stage |
title | Intravitreal Bevacizumab Injection Attenuates Diabetic Retinopathy in Adult Rats with Experimentally Induced Diabetes in the Early Stage |
title_full | Intravitreal Bevacizumab Injection Attenuates Diabetic Retinopathy in Adult Rats with Experimentally Induced Diabetes in the Early Stage |
title_fullStr | Intravitreal Bevacizumab Injection Attenuates Diabetic Retinopathy in Adult Rats with Experimentally Induced Diabetes in the Early Stage |
title_full_unstemmed | Intravitreal Bevacizumab Injection Attenuates Diabetic Retinopathy in Adult Rats with Experimentally Induced Diabetes in the Early Stage |
title_short | Intravitreal Bevacizumab Injection Attenuates Diabetic Retinopathy in Adult Rats with Experimentally Induced Diabetes in the Early Stage |
title_sort | intravitreal bevacizumab injection attenuates diabetic retinopathy in adult rats with experimentally induced diabetes in the early stage |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5994287/ https://www.ncbi.nlm.nih.gov/pubmed/29977927 http://dx.doi.org/10.1155/2018/9216791 |
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