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Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response

In eukaryotic cells, organelle-specific stress-response mechanisms are vital for maintaining cellular homeostasis. The Golgi apparatus, an essential organelle of the secretory system, is the major site of protein modification and sorting within a cell and functions as a platform for spatially regula...

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Autores principales: Serebrenik, Yevgeniy V., Hellerschmied, Doris, Toure, Momar, López-Giráldez, Francesc, Brookner, Dennis, Crews, Craig M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5994893/
https://www.ncbi.nlm.nih.gov/pubmed/29851555
http://dx.doi.org/10.1091/mbc.E17-11-0693
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author Serebrenik, Yevgeniy V.
Hellerschmied, Doris
Toure, Momar
López-Giráldez, Francesc
Brookner, Dennis
Crews, Craig M.
author_facet Serebrenik, Yevgeniy V.
Hellerschmied, Doris
Toure, Momar
López-Giráldez, Francesc
Brookner, Dennis
Crews, Craig M.
author_sort Serebrenik, Yevgeniy V.
collection PubMed
description In eukaryotic cells, organelle-specific stress-response mechanisms are vital for maintaining cellular homeostasis. The Golgi apparatus, an essential organelle of the secretory system, is the major site of protein modification and sorting within a cell and functions as a platform for spatially regulated signaling. Golgi homeostasis mechanisms that regulate organelle structure and ensure precise processing and localization of protein substrates remain poorly understood. Using a chemical biology strategy to induce protein unfolding, we uncover a Golgi-specific transcriptional response. An RNA-sequencing profile of this stress response compared with the current state-of-the-art Golgi stressors, nigericin and xyloside, demonstrates the enhanced precision of Golgi targeting achieved with our system. The data set further reveals previously uncharacterized genes that we find to be essential for Golgi structural integrity. These findings highlight the Golgi’s ability to sense misfolded proteins and establish new aspects of Golgi autoregulation.
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spelling pubmed-59948932018-08-16 Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response Serebrenik, Yevgeniy V. Hellerschmied, Doris Toure, Momar López-Giráldez, Francesc Brookner, Dennis Crews, Craig M. Mol Biol Cell Brief Reports In eukaryotic cells, organelle-specific stress-response mechanisms are vital for maintaining cellular homeostasis. The Golgi apparatus, an essential organelle of the secretory system, is the major site of protein modification and sorting within a cell and functions as a platform for spatially regulated signaling. Golgi homeostasis mechanisms that regulate organelle structure and ensure precise processing and localization of protein substrates remain poorly understood. Using a chemical biology strategy to induce protein unfolding, we uncover a Golgi-specific transcriptional response. An RNA-sequencing profile of this stress response compared with the current state-of-the-art Golgi stressors, nigericin and xyloside, demonstrates the enhanced precision of Golgi targeting achieved with our system. The data set further reveals previously uncharacterized genes that we find to be essential for Golgi structural integrity. These findings highlight the Golgi’s ability to sense misfolded proteins and establish new aspects of Golgi autoregulation. The American Society for Cell Biology 2018-06-01 /pmc/articles/PMC5994893/ /pubmed/29851555 http://dx.doi.org/10.1091/mbc.E17-11-0693 Text en © 2018 Serebrenik, Hellerschmied, et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0/ This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Brief Reports
Serebrenik, Yevgeniy V.
Hellerschmied, Doris
Toure, Momar
López-Giráldez, Francesc
Brookner, Dennis
Crews, Craig M.
Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response
title Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response
title_full Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response
title_fullStr Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response
title_full_unstemmed Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response
title_short Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response
title_sort targeted protein unfolding uncovers a golgi-specific transcriptional stress response
topic Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5994893/
https://www.ncbi.nlm.nih.gov/pubmed/29851555
http://dx.doi.org/10.1091/mbc.E17-11-0693
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