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Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response
In eukaryotic cells, organelle-specific stress-response mechanisms are vital for maintaining cellular homeostasis. The Golgi apparatus, an essential organelle of the secretory system, is the major site of protein modification and sorting within a cell and functions as a platform for spatially regula...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5994893/ https://www.ncbi.nlm.nih.gov/pubmed/29851555 http://dx.doi.org/10.1091/mbc.E17-11-0693 |
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author | Serebrenik, Yevgeniy V. Hellerschmied, Doris Toure, Momar López-Giráldez, Francesc Brookner, Dennis Crews, Craig M. |
author_facet | Serebrenik, Yevgeniy V. Hellerschmied, Doris Toure, Momar López-Giráldez, Francesc Brookner, Dennis Crews, Craig M. |
author_sort | Serebrenik, Yevgeniy V. |
collection | PubMed |
description | In eukaryotic cells, organelle-specific stress-response mechanisms are vital for maintaining cellular homeostasis. The Golgi apparatus, an essential organelle of the secretory system, is the major site of protein modification and sorting within a cell and functions as a platform for spatially regulated signaling. Golgi homeostasis mechanisms that regulate organelle structure and ensure precise processing and localization of protein substrates remain poorly understood. Using a chemical biology strategy to induce protein unfolding, we uncover a Golgi-specific transcriptional response. An RNA-sequencing profile of this stress response compared with the current state-of-the-art Golgi stressors, nigericin and xyloside, demonstrates the enhanced precision of Golgi targeting achieved with our system. The data set further reveals previously uncharacterized genes that we find to be essential for Golgi structural integrity. These findings highlight the Golgi’s ability to sense misfolded proteins and establish new aspects of Golgi autoregulation. |
format | Online Article Text |
id | pubmed-5994893 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-59948932018-08-16 Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response Serebrenik, Yevgeniy V. Hellerschmied, Doris Toure, Momar López-Giráldez, Francesc Brookner, Dennis Crews, Craig M. Mol Biol Cell Brief Reports In eukaryotic cells, organelle-specific stress-response mechanisms are vital for maintaining cellular homeostasis. The Golgi apparatus, an essential organelle of the secretory system, is the major site of protein modification and sorting within a cell and functions as a platform for spatially regulated signaling. Golgi homeostasis mechanisms that regulate organelle structure and ensure precise processing and localization of protein substrates remain poorly understood. Using a chemical biology strategy to induce protein unfolding, we uncover a Golgi-specific transcriptional response. An RNA-sequencing profile of this stress response compared with the current state-of-the-art Golgi stressors, nigericin and xyloside, demonstrates the enhanced precision of Golgi targeting achieved with our system. The data set further reveals previously uncharacterized genes that we find to be essential for Golgi structural integrity. These findings highlight the Golgi’s ability to sense misfolded proteins and establish new aspects of Golgi autoregulation. The American Society for Cell Biology 2018-06-01 /pmc/articles/PMC5994893/ /pubmed/29851555 http://dx.doi.org/10.1091/mbc.E17-11-0693 Text en © 2018 Serebrenik, Hellerschmied, et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0/ This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License. |
spellingShingle | Brief Reports Serebrenik, Yevgeniy V. Hellerschmied, Doris Toure, Momar López-Giráldez, Francesc Brookner, Dennis Crews, Craig M. Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response |
title | Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response |
title_full | Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response |
title_fullStr | Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response |
title_full_unstemmed | Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response |
title_short | Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response |
title_sort | targeted protein unfolding uncovers a golgi-specific transcriptional stress response |
topic | Brief Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5994893/ https://www.ncbi.nlm.nih.gov/pubmed/29851555 http://dx.doi.org/10.1091/mbc.E17-11-0693 |
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