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Platelet activation parameters and platelet-leucocyte-conjugate formation in glioblastoma multiforme patients

Patients with glioblastoma multiforme (GBM) suffer from an increased incidence of vascular thrombotic events. However, key influencing factors of the primary hemostasis have not been characterized in GBM patients to date. Thus, the present study determines the activation level of circulating platele...

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Autores principales: Marx, Sascha, Splittstöhser, Maximilian, Kinnen, Frederik, Moritz, Eileen, Joseph, Christy, Paul, Sebastian, Paland, Heiko, Seifert, Carolin, Marx, Madlen, Böhm, Andreas, Schwedhelm, Edzard, Holzer, Kerstin, Singer, Stephan, Ritter, Christoph A., Bien-Möller, Sandra, Schroeder, Henry W.S., Rauch, Bernhard H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5995223/
https://www.ncbi.nlm.nih.gov/pubmed/29899827
http://dx.doi.org/10.18632/oncotarget.25395
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author Marx, Sascha
Splittstöhser, Maximilian
Kinnen, Frederik
Moritz, Eileen
Joseph, Christy
Paul, Sebastian
Paland, Heiko
Seifert, Carolin
Marx, Madlen
Böhm, Andreas
Schwedhelm, Edzard
Holzer, Kerstin
Singer, Stephan
Ritter, Christoph A.
Bien-Möller, Sandra
Schroeder, Henry W.S.
Rauch, Bernhard H.
author_facet Marx, Sascha
Splittstöhser, Maximilian
Kinnen, Frederik
Moritz, Eileen
Joseph, Christy
Paul, Sebastian
Paland, Heiko
Seifert, Carolin
Marx, Madlen
Böhm, Andreas
Schwedhelm, Edzard
Holzer, Kerstin
Singer, Stephan
Ritter, Christoph A.
Bien-Möller, Sandra
Schroeder, Henry W.S.
Rauch, Bernhard H.
author_sort Marx, Sascha
collection PubMed
description Patients with glioblastoma multiforme (GBM) suffer from an increased incidence of vascular thrombotic events. However, key influencing factors of the primary hemostasis have not been characterized in GBM patients to date. Thus, the present study determines the activation level of circulating platelets in GBM patients, in-vitro reactivity to agonist-induced platelet stimulation and the formation of circulating platelet-leucocyte conjugates as well as the plasma levels of the proinflammatory lipid mediator sphingosine-1-phosphate (S1P). The endogenous thrombin potential (ETP) was determined as global marker for hemostasis. The 21 GBM patients and 21 gender and age matched healthy individuals enrolled in this study did not differ in mean total platelet count. Basal surface expression of platelet CD63 determined by flow cytometry was significantly increased in GBM patients compared to controls as was observed for the concentration of soluble P-selectin in the plasma of GBM patients. While the ETP was not affected, the immunomodulatory lipid S1P was significantly decreased in peripheral blood in GBM. Interestingly, monocyte expression of PSGL-1 (CD162) was decreased in GBM patient blood, possibly explaining the rather decreased formation of platelet-monocyte conjugates. Our study reveals an increased CD63 expression and P-selectin expression/ secretion of circulating platelets in GBM patients. In parallel a down-modulated PSGL-1 expression in circulating monocytes and a trend towards a decreased formation of heterotypic platelet-monocyte conjugates in GBM patients was seen. Whether this and the observed decreased plasma level of the immunomodulatory S1P reflects a systemic anti-inflammatory status needs to be addressed in future studies.
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spelling pubmed-59952232018-06-13 Platelet activation parameters and platelet-leucocyte-conjugate formation in glioblastoma multiforme patients Marx, Sascha Splittstöhser, Maximilian Kinnen, Frederik Moritz, Eileen Joseph, Christy Paul, Sebastian Paland, Heiko Seifert, Carolin Marx, Madlen Böhm, Andreas Schwedhelm, Edzard Holzer, Kerstin Singer, Stephan Ritter, Christoph A. Bien-Möller, Sandra Schroeder, Henry W.S. Rauch, Bernhard H. Oncotarget Research Paper Patients with glioblastoma multiforme (GBM) suffer from an increased incidence of vascular thrombotic events. However, key influencing factors of the primary hemostasis have not been characterized in GBM patients to date. Thus, the present study determines the activation level of circulating platelets in GBM patients, in-vitro reactivity to agonist-induced platelet stimulation and the formation of circulating platelet-leucocyte conjugates as well as the plasma levels of the proinflammatory lipid mediator sphingosine-1-phosphate (S1P). The endogenous thrombin potential (ETP) was determined as global marker for hemostasis. The 21 GBM patients and 21 gender and age matched healthy individuals enrolled in this study did not differ in mean total platelet count. Basal surface expression of platelet CD63 determined by flow cytometry was significantly increased in GBM patients compared to controls as was observed for the concentration of soluble P-selectin in the plasma of GBM patients. While the ETP was not affected, the immunomodulatory lipid S1P was significantly decreased in peripheral blood in GBM. Interestingly, monocyte expression of PSGL-1 (CD162) was decreased in GBM patient blood, possibly explaining the rather decreased formation of platelet-monocyte conjugates. Our study reveals an increased CD63 expression and P-selectin expression/ secretion of circulating platelets in GBM patients. In parallel a down-modulated PSGL-1 expression in circulating monocytes and a trend towards a decreased formation of heterotypic platelet-monocyte conjugates in GBM patients was seen. Whether this and the observed decreased plasma level of the immunomodulatory S1P reflects a systemic anti-inflammatory status needs to be addressed in future studies. Impact Journals LLC 2018-05-25 /pmc/articles/PMC5995223/ /pubmed/29899827 http://dx.doi.org/10.18632/oncotarget.25395 Text en Copyright: © 2018 Marx et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Paper
Marx, Sascha
Splittstöhser, Maximilian
Kinnen, Frederik
Moritz, Eileen
Joseph, Christy
Paul, Sebastian
Paland, Heiko
Seifert, Carolin
Marx, Madlen
Böhm, Andreas
Schwedhelm, Edzard
Holzer, Kerstin
Singer, Stephan
Ritter, Christoph A.
Bien-Möller, Sandra
Schroeder, Henry W.S.
Rauch, Bernhard H.
Platelet activation parameters and platelet-leucocyte-conjugate formation in glioblastoma multiforme patients
title Platelet activation parameters and platelet-leucocyte-conjugate formation in glioblastoma multiforme patients
title_full Platelet activation parameters and platelet-leucocyte-conjugate formation in glioblastoma multiforme patients
title_fullStr Platelet activation parameters and platelet-leucocyte-conjugate formation in glioblastoma multiforme patients
title_full_unstemmed Platelet activation parameters and platelet-leucocyte-conjugate formation in glioblastoma multiforme patients
title_short Platelet activation parameters and platelet-leucocyte-conjugate formation in glioblastoma multiforme patients
title_sort platelet activation parameters and platelet-leucocyte-conjugate formation in glioblastoma multiforme patients
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5995223/
https://www.ncbi.nlm.nih.gov/pubmed/29899827
http://dx.doi.org/10.18632/oncotarget.25395
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