Clinical validation of the Tempus xO assay

We have developed a clinically validated NGS assay that includes tumor, germline and RNA sequencing. We apply this assay to clinical specimens and cell lines, and we demonstrate a clinical sensitivity of 98.4% and positive predictive value of 100% for the clinically actionable variants measured by t...

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Autores principales: Beaubier, Nike, Tell, Robert, Huether, Robert, Bontrager, Martin, Bush, Stephen, Parsons, Jerod, Shah, Kaanan, Baker, Tim, Selkov, Gene, Taxter, Tim, Thomas, Amber, Bettis, Sam, Khan, Aly, Lau, Denise, Lee, Christina, Barber, Matthew, Cieslik, Marcin, Frankenberger, Casey, Franzen, Amy, Weiner, Ali, Palmer, Gary, Lonigro, Robert, Robinson, Dan, Wu, Yi-Mi, Cao, Xuhong, Lefkofsky, Eric, Chinnaiyan, Arul, White, Kevin P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2018
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5995233/
https://www.ncbi.nlm.nih.gov/pubmed/29899824
http://dx.doi.org/10.18632/oncotarget.25381
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author Beaubier, Nike
Tell, Robert
Huether, Robert
Bontrager, Martin
Bush, Stephen
Parsons, Jerod
Shah, Kaanan
Baker, Tim
Selkov, Gene
Taxter, Tim
Thomas, Amber
Bettis, Sam
Khan, Aly
Lau, Denise
Lee, Christina
Barber, Matthew
Cieslik, Marcin
Frankenberger, Casey
Franzen, Amy
Weiner, Ali
Palmer, Gary
Lonigro, Robert
Robinson, Dan
Wu, Yi-Mi
Cao, Xuhong
Lefkofsky, Eric
Chinnaiyan, Arul
White, Kevin P.
author_facet Beaubier, Nike
Tell, Robert
Huether, Robert
Bontrager, Martin
Bush, Stephen
Parsons, Jerod
Shah, Kaanan
Baker, Tim
Selkov, Gene
Taxter, Tim
Thomas, Amber
Bettis, Sam
Khan, Aly
Lau, Denise
Lee, Christina
Barber, Matthew
Cieslik, Marcin
Frankenberger, Casey
Franzen, Amy
Weiner, Ali
Palmer, Gary
Lonigro, Robert
Robinson, Dan
Wu, Yi-Mi
Cao, Xuhong
Lefkofsky, Eric
Chinnaiyan, Arul
White, Kevin P.
author_sort Beaubier, Nike
collection PubMed
description We have developed a clinically validated NGS assay that includes tumor, germline and RNA sequencing. We apply this assay to clinical specimens and cell lines, and we demonstrate a clinical sensitivity of 98.4% and positive predictive value of 100% for the clinically actionable variants measured by the assay. We also demonstrate highly accurate copy number measurements and gene rearrangement identification.
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spelling pubmed-59952332018-06-13 Clinical validation of the Tempus xO assay Beaubier, Nike Tell, Robert Huether, Robert Bontrager, Martin Bush, Stephen Parsons, Jerod Shah, Kaanan Baker, Tim Selkov, Gene Taxter, Tim Thomas, Amber Bettis, Sam Khan, Aly Lau, Denise Lee, Christina Barber, Matthew Cieslik, Marcin Frankenberger, Casey Franzen, Amy Weiner, Ali Palmer, Gary Lonigro, Robert Robinson, Dan Wu, Yi-Mi Cao, Xuhong Lefkofsky, Eric Chinnaiyan, Arul White, Kevin P. Oncotarget Research Paper We have developed a clinically validated NGS assay that includes tumor, germline and RNA sequencing. We apply this assay to clinical specimens and cell lines, and we demonstrate a clinical sensitivity of 98.4% and positive predictive value of 100% for the clinically actionable variants measured by the assay. We also demonstrate highly accurate copy number measurements and gene rearrangement identification. Impact Journals LLC 2018-05-25 /pmc/articles/PMC5995233/ /pubmed/29899824 http://dx.doi.org/10.18632/oncotarget.25381 Text en Copyright: © 2018 Beaubier et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Paper
Beaubier, Nike
Tell, Robert
Huether, Robert
Bontrager, Martin
Bush, Stephen
Parsons, Jerod
Shah, Kaanan
Baker, Tim
Selkov, Gene
Taxter, Tim
Thomas, Amber
Bettis, Sam
Khan, Aly
Lau, Denise
Lee, Christina
Barber, Matthew
Cieslik, Marcin
Frankenberger, Casey
Franzen, Amy
Weiner, Ali
Palmer, Gary
Lonigro, Robert
Robinson, Dan
Wu, Yi-Mi
Cao, Xuhong
Lefkofsky, Eric
Chinnaiyan, Arul
White, Kevin P.
Clinical validation of the Tempus xO assay
title Clinical validation of the Tempus xO assay
title_full Clinical validation of the Tempus xO assay
title_fullStr Clinical validation of the Tempus xO assay
title_full_unstemmed Clinical validation of the Tempus xO assay
title_short Clinical validation of the Tempus xO assay
title_sort clinical validation of the tempus xo assay
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5995233/
https://www.ncbi.nlm.nih.gov/pubmed/29899824
http://dx.doi.org/10.18632/oncotarget.25381
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