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Plastid Envelope-Localized Proteins Exhibit a Stochastic Spatiotemporal Relationship to Stromules

Plastids in the viridiplantae sporadically form thin tubules called stromules that increase the interactive surface between the plastid and the surrounding cytoplasm. Several recent publications that report observations of certain proteins localizing to the extensions have then used the observations...

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Autores principales: Delfosse, Kathleen, Wozny, Michael R., Barton, Kiah A., Mathur, Neeta, Griffiths, Nigel, Mathur, Jaideep
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5995270/
https://www.ncbi.nlm.nih.gov/pubmed/29915611
http://dx.doi.org/10.3389/fpls.2018.00754
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author Delfosse, Kathleen
Wozny, Michael R.
Barton, Kiah A.
Mathur, Neeta
Griffiths, Nigel
Mathur, Jaideep
author_facet Delfosse, Kathleen
Wozny, Michael R.
Barton, Kiah A.
Mathur, Neeta
Griffiths, Nigel
Mathur, Jaideep
author_sort Delfosse, Kathleen
collection PubMed
description Plastids in the viridiplantae sporadically form thin tubules called stromules that increase the interactive surface between the plastid and the surrounding cytoplasm. Several recent publications that report observations of certain proteins localizing to the extensions have then used the observations to suggest stromule-specific functions. The mechanisms by which specific localizations on these transient and sporadically formed extensions might occur remain unclear. Previous studies have yet to address the spatiotemporal relationship between a particular protein localization pattern and its distribution on an extended stromules and/or the plastid body. Here, we have used discrete protein patches found in several transgenic plants as fiducial markers to investigate this relationship. While we consider the inner plastid envelope-membrane localized protein patches of the GLUCOSE 6-PHOSPHATE/PHOSPHATE TRANSLOCATOR1 and the TRIOSE-PHOSPHATE/ PHOSPHATE TRANSLOCATOR 1 as artifacts of fluorescent fusion protein over-expression, stromule formation is not compromised in the respective stable transgenic lines that maintain normal growth and development. Our analysis of chloroplasts in the transgenic lines in the Arabidopsis Columbia background, and in the arc6 mutant, under stromule-inducing conditions shows that the possibility of finding a particular protein-enriched domain on an extended stromule or on a region of the main plastid body is stochastic. Our observations provide insights on the behavior of chloroplasts, the relationship between stromules and the plastid-body and strongly challenge claims of stromule-specific functions based solely upon protein localization to plastid extensions. ONE SENTENCE SUMMARY: Observations of the spatiotemporal relationship between plastid envelope localized fluorescent protein fusions of two sugar-phosphate transporters and stromules suggest a stochastic rather than specific localization pattern that questions the idea of independent functions for stromules.
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spelling pubmed-59952702018-06-18 Plastid Envelope-Localized Proteins Exhibit a Stochastic Spatiotemporal Relationship to Stromules Delfosse, Kathleen Wozny, Michael R. Barton, Kiah A. Mathur, Neeta Griffiths, Nigel Mathur, Jaideep Front Plant Sci Plant Science Plastids in the viridiplantae sporadically form thin tubules called stromules that increase the interactive surface between the plastid and the surrounding cytoplasm. Several recent publications that report observations of certain proteins localizing to the extensions have then used the observations to suggest stromule-specific functions. The mechanisms by which specific localizations on these transient and sporadically formed extensions might occur remain unclear. Previous studies have yet to address the spatiotemporal relationship between a particular protein localization pattern and its distribution on an extended stromules and/or the plastid body. Here, we have used discrete protein patches found in several transgenic plants as fiducial markers to investigate this relationship. While we consider the inner plastid envelope-membrane localized protein patches of the GLUCOSE 6-PHOSPHATE/PHOSPHATE TRANSLOCATOR1 and the TRIOSE-PHOSPHATE/ PHOSPHATE TRANSLOCATOR 1 as artifacts of fluorescent fusion protein over-expression, stromule formation is not compromised in the respective stable transgenic lines that maintain normal growth and development. Our analysis of chloroplasts in the transgenic lines in the Arabidopsis Columbia background, and in the arc6 mutant, under stromule-inducing conditions shows that the possibility of finding a particular protein-enriched domain on an extended stromule or on a region of the main plastid body is stochastic. Our observations provide insights on the behavior of chloroplasts, the relationship between stromules and the plastid-body and strongly challenge claims of stromule-specific functions based solely upon protein localization to plastid extensions. ONE SENTENCE SUMMARY: Observations of the spatiotemporal relationship between plastid envelope localized fluorescent protein fusions of two sugar-phosphate transporters and stromules suggest a stochastic rather than specific localization pattern that questions the idea of independent functions for stromules. Frontiers Media S.A. 2018-06-04 /pmc/articles/PMC5995270/ /pubmed/29915611 http://dx.doi.org/10.3389/fpls.2018.00754 Text en Copyright © 2018 Delfosse, Wozny, Barton, Mathur, Griffiths and Mathur. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Delfosse, Kathleen
Wozny, Michael R.
Barton, Kiah A.
Mathur, Neeta
Griffiths, Nigel
Mathur, Jaideep
Plastid Envelope-Localized Proteins Exhibit a Stochastic Spatiotemporal Relationship to Stromules
title Plastid Envelope-Localized Proteins Exhibit a Stochastic Spatiotemporal Relationship to Stromules
title_full Plastid Envelope-Localized Proteins Exhibit a Stochastic Spatiotemporal Relationship to Stromules
title_fullStr Plastid Envelope-Localized Proteins Exhibit a Stochastic Spatiotemporal Relationship to Stromules
title_full_unstemmed Plastid Envelope-Localized Proteins Exhibit a Stochastic Spatiotemporal Relationship to Stromules
title_short Plastid Envelope-Localized Proteins Exhibit a Stochastic Spatiotemporal Relationship to Stromules
title_sort plastid envelope-localized proteins exhibit a stochastic spatiotemporal relationship to stromules
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5995270/
https://www.ncbi.nlm.nih.gov/pubmed/29915611
http://dx.doi.org/10.3389/fpls.2018.00754
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