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Effect of AQP-5 silencing by siRNA interference on chemosensitivity of breast cancer cells
OBJECTIVES: Based on the functionality of AQP-5 characterized in various physiological processes, our study aimed to investigate the effect of AQP-5 silencing by siRNA interference on chemosensitivity of breast cancer cells. MATERIALS AND METHODS: The expression levels of AQP-5 mRNA in different exp...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5995417/ https://www.ncbi.nlm.nih.gov/pubmed/29922074 http://dx.doi.org/10.2147/OTT.S160313 |
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author | Li, Xueqing Pei, Bing Wang, Hui Tang, Chenghui Zhu, Wei Jin, Feng |
author_facet | Li, Xueqing Pei, Bing Wang, Hui Tang, Chenghui Zhu, Wei Jin, Feng |
author_sort | Li, Xueqing |
collection | PubMed |
description | OBJECTIVES: Based on the functionality of AQP-5 characterized in various physiological processes, our study aimed to investigate the effect of AQP-5 silencing by siRNA interference on chemosensitivity of breast cancer cells. MATERIALS AND METHODS: The expression levels of AQP-5 mRNA in different experimental groups were measured by reverse transcription PCR. The chemosensitivity of the cells to adriamycin (ADR) was detected by a CCK-8 kit. Cell invasion, migration, and apoptosis were assessed. Western blot was used to detect the expression levels of AQP-5, drug resistance-related protein, and apoptosis-related protein. RESULTS: The expression level of AQP-5 in MCF-7/ADR cells was significantly reduced by AQP-5 siRNA transfection. The invasion and migration were significantly reduced in MCF-7/ADR after AQP-5 siRNA interference. AQP-5 silencing significantly increased the chemosensitivity of MCF-7/ADR cells to ADR and activated caspase-dependent apoptosis in MCF-7/ADR cells. AQP-5 silencing also decreased the expression levels of drug resistance-related proteins (P-gp and GST-π). CONCLUSION: The inhibition of AQP-5 expression may reverse the drug resistance and enhance the chemosensitivity of breast cancer cells. |
format | Online Article Text |
id | pubmed-5995417 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-59954172018-06-19 Effect of AQP-5 silencing by siRNA interference on chemosensitivity of breast cancer cells Li, Xueqing Pei, Bing Wang, Hui Tang, Chenghui Zhu, Wei Jin, Feng Onco Targets Ther Original Research OBJECTIVES: Based on the functionality of AQP-5 characterized in various physiological processes, our study aimed to investigate the effect of AQP-5 silencing by siRNA interference on chemosensitivity of breast cancer cells. MATERIALS AND METHODS: The expression levels of AQP-5 mRNA in different experimental groups were measured by reverse transcription PCR. The chemosensitivity of the cells to adriamycin (ADR) was detected by a CCK-8 kit. Cell invasion, migration, and apoptosis were assessed. Western blot was used to detect the expression levels of AQP-5, drug resistance-related protein, and apoptosis-related protein. RESULTS: The expression level of AQP-5 in MCF-7/ADR cells was significantly reduced by AQP-5 siRNA transfection. The invasion and migration were significantly reduced in MCF-7/ADR after AQP-5 siRNA interference. AQP-5 silencing significantly increased the chemosensitivity of MCF-7/ADR cells to ADR and activated caspase-dependent apoptosis in MCF-7/ADR cells. AQP-5 silencing also decreased the expression levels of drug resistance-related proteins (P-gp and GST-π). CONCLUSION: The inhibition of AQP-5 expression may reverse the drug resistance and enhance the chemosensitivity of breast cancer cells. Dove Medical Press 2018-06-06 /pmc/articles/PMC5995417/ /pubmed/29922074 http://dx.doi.org/10.2147/OTT.S160313 Text en © 2018 Li et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Li, Xueqing Pei, Bing Wang, Hui Tang, Chenghui Zhu, Wei Jin, Feng Effect of AQP-5 silencing by siRNA interference on chemosensitivity of breast cancer cells |
title | Effect of AQP-5 silencing by siRNA interference on chemosensitivity of breast cancer cells |
title_full | Effect of AQP-5 silencing by siRNA interference on chemosensitivity of breast cancer cells |
title_fullStr | Effect of AQP-5 silencing by siRNA interference on chemosensitivity of breast cancer cells |
title_full_unstemmed | Effect of AQP-5 silencing by siRNA interference on chemosensitivity of breast cancer cells |
title_short | Effect of AQP-5 silencing by siRNA interference on chemosensitivity of breast cancer cells |
title_sort | effect of aqp-5 silencing by sirna interference on chemosensitivity of breast cancer cells |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5995417/ https://www.ncbi.nlm.nih.gov/pubmed/29922074 http://dx.doi.org/10.2147/OTT.S160313 |
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