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Candida albicans Cdc15 is essential for mitotic exit and cytokinesis

Candida albicans displays a variety of morphological forms, and the ability to switch forms must be linked with cell cycle control. In budding yeast the Mitotic Exit Network (MEN) acts to drive mitotic exit and signal for cytokinesis and cell separation. However, previous reports on the MEN in C. al...

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Autor principal: Bates, Steven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5995815/
https://www.ncbi.nlm.nih.gov/pubmed/29891974
http://dx.doi.org/10.1038/s41598-018-27157-y
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author Bates, Steven
author_facet Bates, Steven
author_sort Bates, Steven
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description Candida albicans displays a variety of morphological forms, and the ability to switch forms must be linked with cell cycle control. In budding yeast the Mitotic Exit Network (MEN) acts to drive mitotic exit and signal for cytokinesis and cell separation. However, previous reports on the MEN in C. albicans have raised questions on its role in this organism, with the components analysed to date demonstrating differing levels of importance in the processes of mitotic exit, cytokinesis and cell separation. This work focuses on the role of the Cdc15 kinase in C. albicans and demonstrates that, similar to Saccharomyces cerevisiae, it plays an essential role in signalling for mitotic exit and cytokinesis. Cells depleted of Cdc15 developed into elongated filaments, a common response to cell cycle arrest in C. albicans. These filaments emerged exclusively from large budded cells, contained two nuclear bodies and exhibited a hyper-extended spindle, all characteristic of these cells failing to exit mitosis. Furthermore these filaments displayed a clear cytokinesis defect, and CDC15 over-expression led to aberrant cell separation following hyphal morphogenesis. Together, these results are consistent with Cdc15 playing an essential role in signalling for mitotic exit, cytokinesis and cell separation in C. albicans.
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spelling pubmed-59958152018-06-21 Candida albicans Cdc15 is essential for mitotic exit and cytokinesis Bates, Steven Sci Rep Article Candida albicans displays a variety of morphological forms, and the ability to switch forms must be linked with cell cycle control. In budding yeast the Mitotic Exit Network (MEN) acts to drive mitotic exit and signal for cytokinesis and cell separation. However, previous reports on the MEN in C. albicans have raised questions on its role in this organism, with the components analysed to date demonstrating differing levels of importance in the processes of mitotic exit, cytokinesis and cell separation. This work focuses on the role of the Cdc15 kinase in C. albicans and demonstrates that, similar to Saccharomyces cerevisiae, it plays an essential role in signalling for mitotic exit and cytokinesis. Cells depleted of Cdc15 developed into elongated filaments, a common response to cell cycle arrest in C. albicans. These filaments emerged exclusively from large budded cells, contained two nuclear bodies and exhibited a hyper-extended spindle, all characteristic of these cells failing to exit mitosis. Furthermore these filaments displayed a clear cytokinesis defect, and CDC15 over-expression led to aberrant cell separation following hyphal morphogenesis. Together, these results are consistent with Cdc15 playing an essential role in signalling for mitotic exit, cytokinesis and cell separation in C. albicans. Nature Publishing Group UK 2018-06-11 /pmc/articles/PMC5995815/ /pubmed/29891974 http://dx.doi.org/10.1038/s41598-018-27157-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Bates, Steven
Candida albicans Cdc15 is essential for mitotic exit and cytokinesis
title Candida albicans Cdc15 is essential for mitotic exit and cytokinesis
title_full Candida albicans Cdc15 is essential for mitotic exit and cytokinesis
title_fullStr Candida albicans Cdc15 is essential for mitotic exit and cytokinesis
title_full_unstemmed Candida albicans Cdc15 is essential for mitotic exit and cytokinesis
title_short Candida albicans Cdc15 is essential for mitotic exit and cytokinesis
title_sort candida albicans cdc15 is essential for mitotic exit and cytokinesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5995815/
https://www.ncbi.nlm.nih.gov/pubmed/29891974
http://dx.doi.org/10.1038/s41598-018-27157-y
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