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Hepatic Metabolomics Investigation in Acute and Chronic Murine Toxoplasmosis
Toxoplasma gondii poses a great threat to human health, with no approved vaccine available for the treatment of T. gondii infection. T. gondii infections are not limited to the brain, and may also affect other organs especially the liver. Identification of host liver molecules or pathways involved i...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5996072/ https://www.ncbi.nlm.nih.gov/pubmed/29922602 http://dx.doi.org/10.3389/fcimb.2018.00189 |
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author | Chen, Xiao-Qing Elsheikha, Hany M. Hu, Rui-Si Hu, Gui-Xue Guo, Shu-Ling Zhou, Chun-Xue Zhu, Xing-Quan |
author_facet | Chen, Xiao-Qing Elsheikha, Hany M. Hu, Rui-Si Hu, Gui-Xue Guo, Shu-Ling Zhou, Chun-Xue Zhu, Xing-Quan |
author_sort | Chen, Xiao-Qing |
collection | PubMed |
description | Toxoplasma gondii poses a great threat to human health, with no approved vaccine available for the treatment of T. gondii infection. T. gondii infections are not limited to the brain, and may also affect other organs especially the liver. Identification of host liver molecules or pathways involved in T. gondii replication process may lead to the discovery of novel anti-T. gondii targets. Here, we analyzed the metabolic profile of the liver of mice on 11 and 30 days postinfection (dpi) with type II T. gondii Pru strain. Global metabolomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS) identified 389 significant metabolites from acutely infected mice; and 368 from chronically infected mice, when compared with control mice. Multivariate statistical analysis revealed distinct metabolic signatures from acutely infected, chronically infected and control mice. Infection influenced several metabolic processes, in particular those for lipids and amino acids. Metabolic pathways, such as steroid hormone biosynthesis, primary bile acid biosynthesis, bile secretion, and biosynthesis of unsaturated fatty acids were perturbed during the whole infection process, particularly during the acute stage of infection. The present results provide insight into hepatic metabolic changes that occur in BALB/c mice during acute and chronic T. gondii infection. |
format | Online Article Text |
id | pubmed-5996072 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-59960722018-06-19 Hepatic Metabolomics Investigation in Acute and Chronic Murine Toxoplasmosis Chen, Xiao-Qing Elsheikha, Hany M. Hu, Rui-Si Hu, Gui-Xue Guo, Shu-Ling Zhou, Chun-Xue Zhu, Xing-Quan Front Cell Infect Microbiol Microbiology Toxoplasma gondii poses a great threat to human health, with no approved vaccine available for the treatment of T. gondii infection. T. gondii infections are not limited to the brain, and may also affect other organs especially the liver. Identification of host liver molecules or pathways involved in T. gondii replication process may lead to the discovery of novel anti-T. gondii targets. Here, we analyzed the metabolic profile of the liver of mice on 11 and 30 days postinfection (dpi) with type II T. gondii Pru strain. Global metabolomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS) identified 389 significant metabolites from acutely infected mice; and 368 from chronically infected mice, when compared with control mice. Multivariate statistical analysis revealed distinct metabolic signatures from acutely infected, chronically infected and control mice. Infection influenced several metabolic processes, in particular those for lipids and amino acids. Metabolic pathways, such as steroid hormone biosynthesis, primary bile acid biosynthesis, bile secretion, and biosynthesis of unsaturated fatty acids were perturbed during the whole infection process, particularly during the acute stage of infection. The present results provide insight into hepatic metabolic changes that occur in BALB/c mice during acute and chronic T. gondii infection. Frontiers Media S.A. 2018-06-05 /pmc/articles/PMC5996072/ /pubmed/29922602 http://dx.doi.org/10.3389/fcimb.2018.00189 Text en Copyright © 2018 Chen, Elsheikha, Hu, Hu, Guo, Zhou and Zhu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Chen, Xiao-Qing Elsheikha, Hany M. Hu, Rui-Si Hu, Gui-Xue Guo, Shu-Ling Zhou, Chun-Xue Zhu, Xing-Quan Hepatic Metabolomics Investigation in Acute and Chronic Murine Toxoplasmosis |
title | Hepatic Metabolomics Investigation in Acute and Chronic Murine Toxoplasmosis |
title_full | Hepatic Metabolomics Investigation in Acute and Chronic Murine Toxoplasmosis |
title_fullStr | Hepatic Metabolomics Investigation in Acute and Chronic Murine Toxoplasmosis |
title_full_unstemmed | Hepatic Metabolomics Investigation in Acute and Chronic Murine Toxoplasmosis |
title_short | Hepatic Metabolomics Investigation in Acute and Chronic Murine Toxoplasmosis |
title_sort | hepatic metabolomics investigation in acute and chronic murine toxoplasmosis |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5996072/ https://www.ncbi.nlm.nih.gov/pubmed/29922602 http://dx.doi.org/10.3389/fcimb.2018.00189 |
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