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Dissecting Community Structure in Wild Blueberry Root and Soil Microbiome

A complex network of functions and symbiotic interactions between a eukaryotic host and its microbiome is a the foundation of the ecological unit holobiont. However, little is known about how the non-fungal eukaryotic microorganisms fit in this complex network of host–microbiome interactions. In thi...

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Autores principales: Yurgel, Svetlana N., Douglas, Gavin M., Dusault, Ashley, Percival, David, Langille, Morgan G. I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5996171/
https://www.ncbi.nlm.nih.gov/pubmed/29922264
http://dx.doi.org/10.3389/fmicb.2018.01187
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author Yurgel, Svetlana N.
Douglas, Gavin M.
Dusault, Ashley
Percival, David
Langille, Morgan G. I.
author_facet Yurgel, Svetlana N.
Douglas, Gavin M.
Dusault, Ashley
Percival, David
Langille, Morgan G. I.
author_sort Yurgel, Svetlana N.
collection PubMed
description A complex network of functions and symbiotic interactions between a eukaryotic host and its microbiome is a the foundation of the ecological unit holobiont. However, little is known about how the non-fungal eukaryotic microorganisms fit in this complex network of host–microbiome interactions. In this study, we employed a unique wild blueberry ecosystem to evaluate plant-associated microbiota, encompassing both eukaryotic and bacterial communities. We found that, while soil microbiome serves as a foundation for root microbiome, plant-influenced species sorting had stronger effect on eukaryotes than on bacteria. Our study identified several fungal and protist taxa, which are correlated with decreased fruit production in wild blueberry agricultural ecosystems. The specific effect of species sorting in root microbiome resulted in an increase in relative abundance of fungi adapted to plant-associated life-style, while the relative abundance of non-fungal eukaryotes was decreased along the soil-endosphere continuum in the root, probably because of low adaptation of these microorganisms to host–plant defense responses. Analysis of community correlation networks indicated that bacterial and eukaryotic interactions became more complex along the soil-endosphere continuum and, in addition to extensive mutualistic interactions, co-exclusion also played an important role in shaping wild blueberry associated microbiome. Our study identified several potential hub taxa with important roles in soil fertility and/or plant–microbe interaction, suggesting the key role of these taxa in the interconnection between soils and plant health and overall microbial community structure. This study also provides a comprehensive view of the role of non-fungal eukaryotes in soil ecosystem.
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spelling pubmed-59961712018-06-19 Dissecting Community Structure in Wild Blueberry Root and Soil Microbiome Yurgel, Svetlana N. Douglas, Gavin M. Dusault, Ashley Percival, David Langille, Morgan G. I. Front Microbiol Microbiology A complex network of functions and symbiotic interactions between a eukaryotic host and its microbiome is a the foundation of the ecological unit holobiont. However, little is known about how the non-fungal eukaryotic microorganisms fit in this complex network of host–microbiome interactions. In this study, we employed a unique wild blueberry ecosystem to evaluate plant-associated microbiota, encompassing both eukaryotic and bacterial communities. We found that, while soil microbiome serves as a foundation for root microbiome, plant-influenced species sorting had stronger effect on eukaryotes than on bacteria. Our study identified several fungal and protist taxa, which are correlated with decreased fruit production in wild blueberry agricultural ecosystems. The specific effect of species sorting in root microbiome resulted in an increase in relative abundance of fungi adapted to plant-associated life-style, while the relative abundance of non-fungal eukaryotes was decreased along the soil-endosphere continuum in the root, probably because of low adaptation of these microorganisms to host–plant defense responses. Analysis of community correlation networks indicated that bacterial and eukaryotic interactions became more complex along the soil-endosphere continuum and, in addition to extensive mutualistic interactions, co-exclusion also played an important role in shaping wild blueberry associated microbiome. Our study identified several potential hub taxa with important roles in soil fertility and/or plant–microbe interaction, suggesting the key role of these taxa in the interconnection between soils and plant health and overall microbial community structure. This study also provides a comprehensive view of the role of non-fungal eukaryotes in soil ecosystem. Frontiers Media S.A. 2018-06-06 /pmc/articles/PMC5996171/ /pubmed/29922264 http://dx.doi.org/10.3389/fmicb.2018.01187 Text en Copyright © 2018 Yurgel, Douglas, Dusault, Percival and Langille. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Yurgel, Svetlana N.
Douglas, Gavin M.
Dusault, Ashley
Percival, David
Langille, Morgan G. I.
Dissecting Community Structure in Wild Blueberry Root and Soil Microbiome
title Dissecting Community Structure in Wild Blueberry Root and Soil Microbiome
title_full Dissecting Community Structure in Wild Blueberry Root and Soil Microbiome
title_fullStr Dissecting Community Structure in Wild Blueberry Root and Soil Microbiome
title_full_unstemmed Dissecting Community Structure in Wild Blueberry Root and Soil Microbiome
title_short Dissecting Community Structure in Wild Blueberry Root and Soil Microbiome
title_sort dissecting community structure in wild blueberry root and soil microbiome
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5996171/
https://www.ncbi.nlm.nih.gov/pubmed/29922264
http://dx.doi.org/10.3389/fmicb.2018.01187
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