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Identification and Characterization of Neuropeptides by Transcriptome and Proteome Analyses in a Bivalve Mollusc Patinopecten yessoensis

Neuropeptides play essential roles in regulation of reproduction and growth in marine molluscs. But their function in marine bivalves – a group of animals of commercial importance – is largely unexplored due to the lack of systematic identification of these molecules. In this study, we sequenced and...

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Autores principales: Zhang, Meiwei, Wang, Yangfan, Li, Yangping, Li, Wanru, Li, Ruojiao, Xie, Xinran, Wang, Shi, Hu, Xiaoli, Zhang, Lingling, Bao, Zhenmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5996578/
https://www.ncbi.nlm.nih.gov/pubmed/29922332
http://dx.doi.org/10.3389/fgene.2018.00197
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author Zhang, Meiwei
Wang, Yangfan
Li, Yangping
Li, Wanru
Li, Ruojiao
Xie, Xinran
Wang, Shi
Hu, Xiaoli
Zhang, Lingling
Bao, Zhenmin
author_facet Zhang, Meiwei
Wang, Yangfan
Li, Yangping
Li, Wanru
Li, Ruojiao
Xie, Xinran
Wang, Shi
Hu, Xiaoli
Zhang, Lingling
Bao, Zhenmin
author_sort Zhang, Meiwei
collection PubMed
description Neuropeptides play essential roles in regulation of reproduction and growth in marine molluscs. But their function in marine bivalves – a group of animals of commercial importance – is largely unexplored due to the lack of systematic identification of these molecules. In this study, we sequenced and analyzed the transcriptome of nerve ganglia of Yesso scallop Patinopecten yessoensis, from which 63 neuropeptide genes were identified based on BLAST and de novo prediction approaches, and 31 were confirmed by proteomic analysis using the liquid chromatography-tandem mass spectrometry (LC-MS/MS). Fifty genes encode known neuropeptide precursors, of which 20 commonly exist in bilaterians and 30 are protostome specific. Three neuropeptides that have not yet been reported in bivalves were identified, including calcitonin/DH31, lymnokinin and pleurin. Characterization of glycoprotein hormones, insulin-like peptides, allatostatins, RFamides, and some reproduction, cardioactivity or feeding related neuropeptides reveals scallop neuropeptides have conserved molluscan neuropeptide domains, but some (e.g., GPB5, APGWamide and ELH) are characterized with bivalve-specific features. Thirteen potentially novel neuropeptides were identified, including 10 that may also exist in other protostomes, and 3 (GNamide, LRYamide, and Vamide) that may be scallop specific. In addition, we found neuropeptides potentially related to scallop shell growth and eye functioning. This study represents the first comprehensive identification of neuropeptides in scallop, and would contribute to a complete understanding on the roles of various neuropeptides in endocrine regulation in bivalve molluscs.
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spelling pubmed-59965782018-06-19 Identification and Characterization of Neuropeptides by Transcriptome and Proteome Analyses in a Bivalve Mollusc Patinopecten yessoensis Zhang, Meiwei Wang, Yangfan Li, Yangping Li, Wanru Li, Ruojiao Xie, Xinran Wang, Shi Hu, Xiaoli Zhang, Lingling Bao, Zhenmin Front Genet Genetics Neuropeptides play essential roles in regulation of reproduction and growth in marine molluscs. But their function in marine bivalves – a group of animals of commercial importance – is largely unexplored due to the lack of systematic identification of these molecules. In this study, we sequenced and analyzed the transcriptome of nerve ganglia of Yesso scallop Patinopecten yessoensis, from which 63 neuropeptide genes were identified based on BLAST and de novo prediction approaches, and 31 were confirmed by proteomic analysis using the liquid chromatography-tandem mass spectrometry (LC-MS/MS). Fifty genes encode known neuropeptide precursors, of which 20 commonly exist in bilaterians and 30 are protostome specific. Three neuropeptides that have not yet been reported in bivalves were identified, including calcitonin/DH31, lymnokinin and pleurin. Characterization of glycoprotein hormones, insulin-like peptides, allatostatins, RFamides, and some reproduction, cardioactivity or feeding related neuropeptides reveals scallop neuropeptides have conserved molluscan neuropeptide domains, but some (e.g., GPB5, APGWamide and ELH) are characterized with bivalve-specific features. Thirteen potentially novel neuropeptides were identified, including 10 that may also exist in other protostomes, and 3 (GNamide, LRYamide, and Vamide) that may be scallop specific. In addition, we found neuropeptides potentially related to scallop shell growth and eye functioning. This study represents the first comprehensive identification of neuropeptides in scallop, and would contribute to a complete understanding on the roles of various neuropeptides in endocrine regulation in bivalve molluscs. Frontiers Media S.A. 2018-06-05 /pmc/articles/PMC5996578/ /pubmed/29922332 http://dx.doi.org/10.3389/fgene.2018.00197 Text en Copyright © 2018 Zhang, Wang, Li, Li, Li, Xie, Wang, Hu, Zhang and Bao. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Zhang, Meiwei
Wang, Yangfan
Li, Yangping
Li, Wanru
Li, Ruojiao
Xie, Xinran
Wang, Shi
Hu, Xiaoli
Zhang, Lingling
Bao, Zhenmin
Identification and Characterization of Neuropeptides by Transcriptome and Proteome Analyses in a Bivalve Mollusc Patinopecten yessoensis
title Identification and Characterization of Neuropeptides by Transcriptome and Proteome Analyses in a Bivalve Mollusc Patinopecten yessoensis
title_full Identification and Characterization of Neuropeptides by Transcriptome and Proteome Analyses in a Bivalve Mollusc Patinopecten yessoensis
title_fullStr Identification and Characterization of Neuropeptides by Transcriptome and Proteome Analyses in a Bivalve Mollusc Patinopecten yessoensis
title_full_unstemmed Identification and Characterization of Neuropeptides by Transcriptome and Proteome Analyses in a Bivalve Mollusc Patinopecten yessoensis
title_short Identification and Characterization of Neuropeptides by Transcriptome and Proteome Analyses in a Bivalve Mollusc Patinopecten yessoensis
title_sort identification and characterization of neuropeptides by transcriptome and proteome analyses in a bivalve mollusc patinopecten yessoensis
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5996578/
https://www.ncbi.nlm.nih.gov/pubmed/29922332
http://dx.doi.org/10.3389/fgene.2018.00197
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