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High resolution crystal structure data of human plasma retinol-binding protein (RBP4) bound to retinol and fatty acids

Retinol is transported in vertebrate plasma bound to a protein called retinol-binding protein (RBP4) so far believed to be specific for the vitamin. When the protein is saturated with retinol it binds tightly to another plasma protein, transthyretin while when not saturated with retinol it does not...

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Autores principales: Perduca, Massimiliano, Nicolis, Stefania, Mannucci, Barbara, Galliano, Monica, Monaco, Hugo L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5996608/
https://www.ncbi.nlm.nih.gov/pubmed/29900277
http://dx.doi.org/10.1016/j.dib.2018.03.112
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author Perduca, Massimiliano
Nicolis, Stefania
Mannucci, Barbara
Galliano, Monica
Monaco, Hugo L.
author_facet Perduca, Massimiliano
Nicolis, Stefania
Mannucci, Barbara
Galliano, Monica
Monaco, Hugo L.
author_sort Perduca, Massimiliano
collection PubMed
description Retinol is transported in vertebrate plasma bound to a protein called retinol-binding protein (RBP4) so far believed to be specific for the vitamin. When the protein is saturated with retinol it binds tightly to another plasma protein, transthyretin while when not saturated with retinol it does not bind to TTR (Goodman, 1984). The X-ray structures of human RBP4, holo and devoid of retinol in its binding site are known to resolutions of 2.0 and 2.5 Å (Cowan et al., 1990; Zanotti et al., 1993) [2], [3]. We have shown that RBP4 is not specific for retinol but it is also found in plasma, urine and amniotic fluid bound to fatty acids. Here we present 1.5 Å resolution crystal data on human plasma retinol-binding protein bound to retinol and fatty acids. These are the highest resolution data available in the Protein Data Bank for this protein. For further details and experimental findings please refer to the article “ Human plasma retinol-binding protein (RBP4) is also a fatty acid-binding protein” (Perduca et al., 2018) [4].
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spelling pubmed-59966082018-06-13 High resolution crystal structure data of human plasma retinol-binding protein (RBP4) bound to retinol and fatty acids Perduca, Massimiliano Nicolis, Stefania Mannucci, Barbara Galliano, Monica Monaco, Hugo L. Data Brief Proteomics and Biochemistry    Retinol is transported in vertebrate plasma bound to a protein called retinol-binding protein (RBP4) so far believed to be specific for the vitamin. When the protein is saturated with retinol it binds tightly to another plasma protein, transthyretin while when not saturated with retinol it does not bind to TTR (Goodman, 1984). The X-ray structures of human RBP4, holo and devoid of retinol in its binding site are known to resolutions of 2.0 and 2.5 Å (Cowan et al., 1990; Zanotti et al., 1993) [2], [3]. We have shown that RBP4 is not specific for retinol but it is also found in plasma, urine and amniotic fluid bound to fatty acids. Here we present 1.5 Å resolution crystal data on human plasma retinol-binding protein bound to retinol and fatty acids. These are the highest resolution data available in the Protein Data Bank for this protein. For further details and experimental findings please refer to the article “ Human plasma retinol-binding protein (RBP4) is also a fatty acid-binding protein” (Perduca et al., 2018) [4]. Elsevier 2018-03-29 /pmc/articles/PMC5996608/ /pubmed/29900277 http://dx.doi.org/10.1016/j.dib.2018.03.112 Text en © 2018 Published by Elsevier Inc. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Proteomics and Biochemistry   
Perduca, Massimiliano
Nicolis, Stefania
Mannucci, Barbara
Galliano, Monica
Monaco, Hugo L.
High resolution crystal structure data of human plasma retinol-binding protein (RBP4) bound to retinol and fatty acids
title High resolution crystal structure data of human plasma retinol-binding protein (RBP4) bound to retinol and fatty acids
title_full High resolution crystal structure data of human plasma retinol-binding protein (RBP4) bound to retinol and fatty acids
title_fullStr High resolution crystal structure data of human plasma retinol-binding protein (RBP4) bound to retinol and fatty acids
title_full_unstemmed High resolution crystal structure data of human plasma retinol-binding protein (RBP4) bound to retinol and fatty acids
title_short High resolution crystal structure data of human plasma retinol-binding protein (RBP4) bound to retinol and fatty acids
title_sort high resolution crystal structure data of human plasma retinol-binding protein (rbp4) bound to retinol and fatty acids
topic Proteomics and Biochemistry   
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5996608/
https://www.ncbi.nlm.nih.gov/pubmed/29900277
http://dx.doi.org/10.1016/j.dib.2018.03.112
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