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Study of S100 Immunostaining in Demonstrating Neural Granulomas in Paucibacillary Leprosy
CONTEXT: Neural granulomas are hallmark of leprosy. Challenges faced in diagnosing paucibacillary leprosy include: (i) Difficult visualization of nerve twigs on hematoxylin and eosin (H and E) sections due to their small size and (ii) Paucity of organisms on acid–fast bacilli stain. AIMS: (1) This s...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5996626/ https://www.ncbi.nlm.nih.gov/pubmed/29937557 http://dx.doi.org/10.4103/ijd.IJD_207_17 |
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author | Shenoy, Nandini Nair, Nandakumar Gopinathan |
author_facet | Shenoy, Nandini Nair, Nandakumar Gopinathan |
author_sort | Shenoy, Nandini |
collection | PubMed |
description | CONTEXT: Neural granulomas are hallmark of leprosy. Challenges faced in diagnosing paucibacillary leprosy include: (i) Difficult visualization of nerve twigs on hematoxylin and eosin (H and E) sections due to their small size and (ii) Paucity of organisms on acid–fast bacilli stain. AIMS: (1) This study aimed to test the role of S100 immunostain in demonstrating neural granulomas in skin biopsies of paucibacillary leprosy, (2) to compare morphology of S100 staining of nerves inside granulomas among clinicohistologically defined different types of leprosy, and (3) to test whether the pattern of S100 immunostaining can distinguish nerve fragmentation/destruction from a normal intact nerve in skin biopsy. MATERIALS AND METHODS: Sixty four diagnosed cases of leprosy were included in this study. Five skin biopsies with no significant pathology (for studying intact nerve) and nine nonleprosy cutaneous granulomas were also studied. RESULTS: (i) In demonstrating neural granuloma, sensitivity of H and E was 48.27% and that of S100 was 100%, (ii) Morphology of nerve fragments on S100 stain for cases of leprosy was fragmented and infiltrated in 37, intact and infiltrated in 19, reduced, fragmented, and infiltrated in seven, and absent in one, (iii) There was a significant difference (P <0.001) in the pattern of staining of S100 on intact nerve and nerves involved by granuloma in leprosy, and (iv) The probability to differentiate between leprosy and nonleprosy granuloma was statistically significant (P <0.001). CONCLUSION: S100 immunostaining showed to be an effective adjuvant to histopathology in diagnosing paucibacillary leprosy and differentiating it from nonleprosy cutaneous granuloma. |
format | Online Article Text |
id | pubmed-5996626 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59966262018-06-22 Study of S100 Immunostaining in Demonstrating Neural Granulomas in Paucibacillary Leprosy Shenoy, Nandini Nair, Nandakumar Gopinathan Indian J Dermatol Original Article CONTEXT: Neural granulomas are hallmark of leprosy. Challenges faced in diagnosing paucibacillary leprosy include: (i) Difficult visualization of nerve twigs on hematoxylin and eosin (H and E) sections due to their small size and (ii) Paucity of organisms on acid–fast bacilli stain. AIMS: (1) This study aimed to test the role of S100 immunostain in demonstrating neural granulomas in skin biopsies of paucibacillary leprosy, (2) to compare morphology of S100 staining of nerves inside granulomas among clinicohistologically defined different types of leprosy, and (3) to test whether the pattern of S100 immunostaining can distinguish nerve fragmentation/destruction from a normal intact nerve in skin biopsy. MATERIALS AND METHODS: Sixty four diagnosed cases of leprosy were included in this study. Five skin biopsies with no significant pathology (for studying intact nerve) and nine nonleprosy cutaneous granulomas were also studied. RESULTS: (i) In demonstrating neural granuloma, sensitivity of H and E was 48.27% and that of S100 was 100%, (ii) Morphology of nerve fragments on S100 stain for cases of leprosy was fragmented and infiltrated in 37, intact and infiltrated in 19, reduced, fragmented, and infiltrated in seven, and absent in one, (iii) There was a significant difference (P <0.001) in the pattern of staining of S100 on intact nerve and nerves involved by granuloma in leprosy, and (iv) The probability to differentiate between leprosy and nonleprosy granuloma was statistically significant (P <0.001). CONCLUSION: S100 immunostaining showed to be an effective adjuvant to histopathology in diagnosing paucibacillary leprosy and differentiating it from nonleprosy cutaneous granuloma. Medknow Publications & Media Pvt Ltd 2018 /pmc/articles/PMC5996626/ /pubmed/29937557 http://dx.doi.org/10.4103/ijd.IJD_207_17 Text en Copyright: © 2018 Indian Journal of Dermatology http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Shenoy, Nandini Nair, Nandakumar Gopinathan Study of S100 Immunostaining in Demonstrating Neural Granulomas in Paucibacillary Leprosy |
title | Study of S100 Immunostaining in Demonstrating Neural Granulomas in Paucibacillary Leprosy |
title_full | Study of S100 Immunostaining in Demonstrating Neural Granulomas in Paucibacillary Leprosy |
title_fullStr | Study of S100 Immunostaining in Demonstrating Neural Granulomas in Paucibacillary Leprosy |
title_full_unstemmed | Study of S100 Immunostaining in Demonstrating Neural Granulomas in Paucibacillary Leprosy |
title_short | Study of S100 Immunostaining in Demonstrating Neural Granulomas in Paucibacillary Leprosy |
title_sort | study of s100 immunostaining in demonstrating neural granulomas in paucibacillary leprosy |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5996626/ https://www.ncbi.nlm.nih.gov/pubmed/29937557 http://dx.doi.org/10.4103/ijd.IJD_207_17 |
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