Cargando…

Probing natural substitute for formalin: Comparing honey, sugar, and jaggery syrup as fixatives

BACKGROUND: Since its discovery in 1859, formalin has been considered as the “gold standard” in tissue fixation. As formalin is highly toxic and carcinogenic, the quest for its substitute has started recently. Literature search reveals very sparse studies on natural substitute for formalin. Here, it...

Descripción completa

Detalles Bibliográficos
Autores principales: Bhattacharyya, Amritaksha, Gupta, Bhavana, Singh, Anil, Sah, Kunal, Gupta, Vivek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5996645/
https://www.ncbi.nlm.nih.gov/pubmed/29937654
http://dx.doi.org/10.4103/njms.NJMS_57_17
_version_ 1783330915677634560
author Bhattacharyya, Amritaksha
Gupta, Bhavana
Singh, Anil
Sah, Kunal
Gupta, Vivek
author_facet Bhattacharyya, Amritaksha
Gupta, Bhavana
Singh, Anil
Sah, Kunal
Gupta, Vivek
author_sort Bhattacharyya, Amritaksha
collection PubMed
description BACKGROUND: Since its discovery in 1859, formalin has been considered as the “gold standard” in tissue fixation. As formalin is highly toxic and carcinogenic, the quest for its substitute has started recently. Literature search reveals very sparse studies on natural substitute for formalin. Here, it is an attempt to explore eco-friendly, economical, and readily available natural substance for formalin substitute. AIM: The aim of our study was to evaluate the efficacy of natural fixatives such as honey, sugar, jaggery, and water in comparison to the standard fixative used like formalin. MATERIALS AND METHODS: Fresh goat tissues (tongue) were fixed separately with buffered 10% formalin (positive control), honey, sugar syrup, jaggery syrup, and distilled water (negative control). 24 h fixation was done at room temperature followed by conventional processing and routine H and E staining. The stained sections were assessed for cytoplasmic and nuclear detail by three pathologists under light microscope and were graded accordingly. RESULTS: The results showed statistically significant differences between jaggery with other natural fixatives for both nuclear details and cytoplasmic staining. CONCLUSION: The preservation of tissue by honey, sugar, and jaggery syrup was comparable to that of formalin. Among the three natural fixatives, jaggery syrup excelled. Hence, it can be considered as an equally effective formalin substitute.
format Online
Article
Text
id pubmed-5996645
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Medknow Publications & Media Pvt Ltd
record_format MEDLINE/PubMed
spelling pubmed-59966452018-06-22 Probing natural substitute for formalin: Comparing honey, sugar, and jaggery syrup as fixatives Bhattacharyya, Amritaksha Gupta, Bhavana Singh, Anil Sah, Kunal Gupta, Vivek Natl J Maxillofac Surg Original Article BACKGROUND: Since its discovery in 1859, formalin has been considered as the “gold standard” in tissue fixation. As formalin is highly toxic and carcinogenic, the quest for its substitute has started recently. Literature search reveals very sparse studies on natural substitute for formalin. Here, it is an attempt to explore eco-friendly, economical, and readily available natural substance for formalin substitute. AIM: The aim of our study was to evaluate the efficacy of natural fixatives such as honey, sugar, jaggery, and water in comparison to the standard fixative used like formalin. MATERIALS AND METHODS: Fresh goat tissues (tongue) were fixed separately with buffered 10% formalin (positive control), honey, sugar syrup, jaggery syrup, and distilled water (negative control). 24 h fixation was done at room temperature followed by conventional processing and routine H and E staining. The stained sections were assessed for cytoplasmic and nuclear detail by three pathologists under light microscope and were graded accordingly. RESULTS: The results showed statistically significant differences between jaggery with other natural fixatives for both nuclear details and cytoplasmic staining. CONCLUSION: The preservation of tissue by honey, sugar, and jaggery syrup was comparable to that of formalin. Among the three natural fixatives, jaggery syrup excelled. Hence, it can be considered as an equally effective formalin substitute. Medknow Publications & Media Pvt Ltd 2018 /pmc/articles/PMC5996645/ /pubmed/29937654 http://dx.doi.org/10.4103/njms.NJMS_57_17 Text en Copyright: © 2018 National Journal of Maxillofacial Surgery http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Bhattacharyya, Amritaksha
Gupta, Bhavana
Singh, Anil
Sah, Kunal
Gupta, Vivek
Probing natural substitute for formalin: Comparing honey, sugar, and jaggery syrup as fixatives
title Probing natural substitute for formalin: Comparing honey, sugar, and jaggery syrup as fixatives
title_full Probing natural substitute for formalin: Comparing honey, sugar, and jaggery syrup as fixatives
title_fullStr Probing natural substitute for formalin: Comparing honey, sugar, and jaggery syrup as fixatives
title_full_unstemmed Probing natural substitute for formalin: Comparing honey, sugar, and jaggery syrup as fixatives
title_short Probing natural substitute for formalin: Comparing honey, sugar, and jaggery syrup as fixatives
title_sort probing natural substitute for formalin: comparing honey, sugar, and jaggery syrup as fixatives
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5996645/
https://www.ncbi.nlm.nih.gov/pubmed/29937654
http://dx.doi.org/10.4103/njms.NJMS_57_17
work_keys_str_mv AT bhattacharyyaamritaksha probingnaturalsubstituteforformalincomparinghoneysugarandjaggerysyrupasfixatives
AT guptabhavana probingnaturalsubstituteforformalincomparinghoneysugarandjaggerysyrupasfixatives
AT singhanil probingnaturalsubstituteforformalincomparinghoneysugarandjaggerysyrupasfixatives
AT sahkunal probingnaturalsubstituteforformalincomparinghoneysugarandjaggerysyrupasfixatives
AT guptavivek probingnaturalsubstituteforformalincomparinghoneysugarandjaggerysyrupasfixatives