Cargando…

The role of fetal-maternal microchimerism as a natural-born healer in integrity improvement of maternal damaged kidney

PURPOSE: To identify the fetal stem cell (FSC) response to maternal renal injury with emphasis on renal integrity improvement and Y chromosome detection in damaged maternal kidney. MATERIALS AND METHODS: Eight non-green fluorescent protein (GFP) transgenic Sprague-Dawley rats were mated with GFP-pos...

Descripción completa

Detalles Bibliográficos
Autores principales: Kajbafzadeh, Abdol-Mohammad, Sabetkish, Shabnam, Sabetkish, Nastaran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Urologia 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5996814/
https://www.ncbi.nlm.nih.gov/pubmed/29211403
http://dx.doi.org/10.1590/S1677-5538.IBJU.2017.0324
_version_ 1783330947832217600
author Kajbafzadeh, Abdol-Mohammad
Sabetkish, Shabnam
Sabetkish, Nastaran
author_facet Kajbafzadeh, Abdol-Mohammad
Sabetkish, Shabnam
Sabetkish, Nastaran
author_sort Kajbafzadeh, Abdol-Mohammad
collection PubMed
description PURPOSE: To identify the fetal stem cell (FSC) response to maternal renal injury with emphasis on renal integrity improvement and Y chromosome detection in damaged maternal kidney. MATERIALS AND METHODS: Eight non-green fluorescent protein (GFP) transgenic Sprague-Dawley rats were mated with GFP-positive transgenic male rats. Renal damage was induced on the right kidney at gestational day 11. The same procedure was performed in eight non-pregnant rats as control group. Three months after delivery, right ne- phrectomy was performed in order to evaluate the injured kidney. The fresh perfused kidneys were stained with anti-GFP antibody. Polymerase chain reaction (PCR) assay was also performed for the Y chromosome detection. Cell culture was performed to detect the GFP-positive cells. Technetium-99m-DMSA renal scan and single-photon emission computed tomography (SPECT) were performed after renal damage induction and 3 months later to evaluate the improvement of renal integrity. RESULTS: The presence of FSCs was confirmed by immune histochemical staining as well as immunofluorescent imaging of the damaged part. Gradient PCR of female rat purified DNA demonstrated the presence of Y-chromosome in the damaged maternal kidney. Moreover, the culture of kidney cells showed GPF- positive cells by immuno- fluorescence microscopy. The acute renal scar was repaired and the integrity of dam- aged kidney reached to near normal levels in experimental group as shown in DMSA scan. However, no significant improvement was observed in control group. CONCLUSION: FSC seems to be the main mechanism in repairing of the maternal renal injury during pregnancy as indicated by Y chromosome and GFP-positive cells in the sub-cultured medium.
format Online
Article
Text
id pubmed-5996814
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Sociedade Brasileira de Urologia
record_format MEDLINE/PubMed
spelling pubmed-59968142018-06-13 The role of fetal-maternal microchimerism as a natural-born healer in integrity improvement of maternal damaged kidney Kajbafzadeh, Abdol-Mohammad Sabetkish, Shabnam Sabetkish, Nastaran Int Braz J Urol Original Article PURPOSE: To identify the fetal stem cell (FSC) response to maternal renal injury with emphasis on renal integrity improvement and Y chromosome detection in damaged maternal kidney. MATERIALS AND METHODS: Eight non-green fluorescent protein (GFP) transgenic Sprague-Dawley rats were mated with GFP-positive transgenic male rats. Renal damage was induced on the right kidney at gestational day 11. The same procedure was performed in eight non-pregnant rats as control group. Three months after delivery, right ne- phrectomy was performed in order to evaluate the injured kidney. The fresh perfused kidneys were stained with anti-GFP antibody. Polymerase chain reaction (PCR) assay was also performed for the Y chromosome detection. Cell culture was performed to detect the GFP-positive cells. Technetium-99m-DMSA renal scan and single-photon emission computed tomography (SPECT) were performed after renal damage induction and 3 months later to evaluate the improvement of renal integrity. RESULTS: The presence of FSCs was confirmed by immune histochemical staining as well as immunofluorescent imaging of the damaged part. Gradient PCR of female rat purified DNA demonstrated the presence of Y-chromosome in the damaged maternal kidney. Moreover, the culture of kidney cells showed GPF- positive cells by immuno- fluorescence microscopy. The acute renal scar was repaired and the integrity of dam- aged kidney reached to near normal levels in experimental group as shown in DMSA scan. However, no significant improvement was observed in control group. CONCLUSION: FSC seems to be the main mechanism in repairing of the maternal renal injury during pregnancy as indicated by Y chromosome and GFP-positive cells in the sub-cultured medium. Sociedade Brasileira de Urologia 2018 /pmc/articles/PMC5996814/ /pubmed/29211403 http://dx.doi.org/10.1590/S1677-5538.IBJU.2017.0324 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kajbafzadeh, Abdol-Mohammad
Sabetkish, Shabnam
Sabetkish, Nastaran
The role of fetal-maternal microchimerism as a natural-born healer in integrity improvement of maternal damaged kidney
title The role of fetal-maternal microchimerism as a natural-born healer in integrity improvement of maternal damaged kidney
title_full The role of fetal-maternal microchimerism as a natural-born healer in integrity improvement of maternal damaged kidney
title_fullStr The role of fetal-maternal microchimerism as a natural-born healer in integrity improvement of maternal damaged kidney
title_full_unstemmed The role of fetal-maternal microchimerism as a natural-born healer in integrity improvement of maternal damaged kidney
title_short The role of fetal-maternal microchimerism as a natural-born healer in integrity improvement of maternal damaged kidney
title_sort role of fetal-maternal microchimerism as a natural-born healer in integrity improvement of maternal damaged kidney
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5996814/
https://www.ncbi.nlm.nih.gov/pubmed/29211403
http://dx.doi.org/10.1590/S1677-5538.IBJU.2017.0324
work_keys_str_mv AT kajbafzadehabdolmohammad theroleoffetalmaternalmicrochimerismasanaturalbornhealerinintegrityimprovementofmaternaldamagedkidney
AT sabetkishshabnam theroleoffetalmaternalmicrochimerismasanaturalbornhealerinintegrityimprovementofmaternaldamagedkidney
AT sabetkishnastaran theroleoffetalmaternalmicrochimerismasanaturalbornhealerinintegrityimprovementofmaternaldamagedkidney
AT kajbafzadehabdolmohammad roleoffetalmaternalmicrochimerismasanaturalbornhealerinintegrityimprovementofmaternaldamagedkidney
AT sabetkishshabnam roleoffetalmaternalmicrochimerismasanaturalbornhealerinintegrityimprovementofmaternaldamagedkidney
AT sabetkishnastaran roleoffetalmaternalmicrochimerismasanaturalbornhealerinintegrityimprovementofmaternaldamagedkidney