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Exploring new routes for secretory protein export from the trans-Golgi network

Sorting of soluble proteins for transport to intracellular compartments and for secretion from cells is essential for cell and tissue homeostasis. The trans-Golgi network (TGN) is a major sorting station that sorts secretory proteins into specific carriers to transport them to their final destinatio...

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Detalles Bibliográficos
Autores principales: Pakdel, Mehrshad, von Blume, Julia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5996961/
https://www.ncbi.nlm.nih.gov/pubmed/29382805
http://dx.doi.org/10.1091/mbc.E17-02-0117
Descripción
Sumario:Sorting of soluble proteins for transport to intracellular compartments and for secretion from cells is essential for cell and tissue homeostasis. The trans-Golgi network (TGN) is a major sorting station that sorts secretory proteins into specific carriers to transport them to their final destinations. The sorting of lysosomal hydrolases at the TGN by the mannose 6-phosphate receptor is well understood. The recent discovery of a Ca(2+)-based sorting of secretory cargo at the TGN is beginning to uncover the mechanism by which cells sort secretory cargoes from Golgi residents and cargoes destined to the other cellular compartments. This Ca(2+)-based sorting involves the cytoplasmic actin cytoskeleton, which through membrane anchored Ca(2+) ATPase SPCA1 and the luminal Ca(2+) binding protein Cab45 sorts of a subset of secretory proteins at the TGN. We present this discovery and highlight important challenges that remain unaddressed in the overall pathway of cargo sorting at the TGN.